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Peroxisome Proliferator-Activated Receptors: Nuclear Control of Metabolism¹

Institute of Animal Biology, University of Lausanne, CH-1015 Lausanne, Switzerland

	Abstract

Top Abstract I. Introduction II. Molecular Aspects III. Physiological Aspects IV. Conclusions References

 

I. Introduction

II. Molecular Aspects

A. PPAR isotypes: identity, genomic organization and chromosomal localization

B. DNA binding properties

C. PPAR ligand-binding properties

D. Alternative pathways for PPAR activation

E. PPAR-mediated transactivation properties

III. Physiological Aspects

A. Differential expression of PPAR mRNAs

B. PPAR target genes and functions in fatty acid metabolism

C. PPARs and control of inflammatory responses

D. PPARs and atherosclerosis

E. PPARs and the development of the fetal epidermal permeability barrier

F. PPARs, carcinogenesis, and control of the cell cycle

IV. Conclusions

	I. Introduction

Top Abstract I. Introduction II. Molecular Aspects III. Physiological Aspects IV. Conclusions References

 
THE REGULATION of lipid and carbohydrate metabolism is central to energy homeostasis in higher multicellular organisms. It involves control systems that are sensitive to stimuli such as the availability of food, physical activity, stress, light, and temperature. The coordination of the responses to signals triggered by these stimuli must occur on several levels to ensure a well adapted energy balance, ranging from hypothalamic functions in the brain to the direct control by lipids and carbohydrates of their own fate. Another important role for lipids is the ability of some of their metabolites, such as leukotrienes or prostaglandins, to be secreted and act as potent mediators in many biological processes that participate in the diverse responses to endogenous and exogenous challenges that the organism faces. In this article, we will concentrate on the role of lipids and their derivatives in the genetic control of their own systemic transport, cellular uptake, storage, mobilization, and use. Fine tuning of these metabolic processes is a hallmark of healthy organisms.

Lipid homeostasis depends on factors that are able to transduce metabolic parameters into regulatory events representing the fundamental components of the general control system. Such factors may modulate the catalytic activity of individual enzymes by allosteric interactions, as do citrate and palmitoyl-coenzyme A (CoA), which activate and inhibit the lipogenic enzyme acetyl-CoA carboxylase, respectively. Alternatively, these factors may participate directly in the transcriptional control of genes encoding proteins involved in key metabolic steps. Several transcription factors that sense lipid levels in animal cells have received much attention in recent years. The adipocyte determination and differentiation factor/sterol regulatory element-binding proteins (ADD/SREBPs)² are intracellular membrane-bound transcription factors whose activity is regulated by the cellular sterol content. In situations of sterol depletion, the active portion of SREBPs is released by proteolytic cleavage, enters the cell nucleus, and stimulates transcription of genes participating in three pathways of lipid metabolism: cholesterol biosynthesis, uptake of circulating fatty acids and cholesterol, and fatty acid biosynthesis (1 ). Another class of transcription factors comprises the liver X receptors [LXRs, i.e., NR1H3, according to the unified nomenclature of nuclear hormone receptors (2 )], whose ligands are oxidized derivatives of cholesterol (oxysterols). Analysis of LXR-deficient mice revealed an essential function of this receptor as a major sensor of dietary cholesterol in the liver and an indispensable regulator of cholesterol homeostasis (3 ). Finally, the peroxisome proliferator-activated receptors (PPARs; NR1C) on which this review is focused belong to the steroid/thyroid/retinoid receptor superfamily, like LXRs, and are nuclear lipid-activable receptors that control a variety of genes in several pathways of lipid metabolism, including fatty acid transport, uptake by the cells, intracellular binding and activation, as well as catabolism (ß-oxidation and -oxidation) or storage. In addition to being indeed activated by fatty acids, they respond to fibrate hypolipidemic drugs and to insulin sensitizers. Rapid progress has been made in the exploration of PPAR biology, which indicates new mechanisms for the regulation of lipid metabolism and functions. In this review, we will first describe molecular aspects concerning the genes that encode PPARs, their structure, and their mechanism of action. The second part concentrates on physiological aspects related to PPAR expression, target genes, and functional consequences of their activation, leading the discussion to the most recent developments in the understanding of their possible physiological roles.

	II. Molecular Aspects

Top Abstract I. Introduction II. Molecular Aspects III. Physiological Aspects IV. Conclusions References

 
A. PPAR isotypes: identity, genomic organization, and chromosomal localization
Three related PPAR isotypes have been identified in vertebrates, including Xenopus, mouse, rat, hamster, and human (4 5 6 7 8 9 10 11 12 13 14 15 ). They were named PPAR (NR1C1), PPARß (NR1C2), and PPAR (NR1C3) when the group of three was originally found in Xenopus (15 ), shortly after the characterization of a first PPAR in the mouse (5 ). With respect to this isotype nomenclature established with the Xenopus PPARs, the mammalian PPAR and PPAR were easily identified, while the third isotype was less clearly homologous to PPARß and was alternatively called PPAR, FAAR, or NUCI. Some evidence such as the expression pattern and the ligand pharmacological profile argue for these Xenopus and mammalian isotypes as being homologs. The analysis of the chicken PPAR ß-like isotype also suggests that ß and are indeed homologous since the chicken sequence falls about half way, in terms of similarity, between that of Xenopus and mammals (K. Umesono, personal communication). Below, we refer to this third isotype as PPARß until additional data provide a final answer to this still open question of isotype identity.

Phylogenetic studies have shown that PPARs form a subfamily of the nuclear receptor superfamily, along with the receptors for thyroid hormone, retinoic acid (RA), vitamin D, ecdysone, and the orphan receptors Rev-ErbA (=ear1; NR1D1) and E75 (NR1D3, from Drosophila), the two latter being the closest relatives of the PPARs (16 ). The ancestral genes in this subfamily appeared more than 500 million years ago (17 ), and a more recent second period saw the duplication of the ancestral thyroid hormone receptor (TR) gene into two genes, TR (NR1A1) and TRß (NR1A2), and of the ancestral retinoic acid receptor (RAR) gene into three genes, RAR (NR1B1), RARß (NR1B2), and RAR (NR1B3). Similarly, the three PPAR loci, , ß, and , appeared during this second period (16 ). Although it is not known whether the duplication events that produced the isotypes occurred exactly at the same time for the three receptors, they were likely contemporaneous to the appearance of the early vertebrates (18 ), as is indeed suggested by the chromosomal location of the TR, PPAR, and RAR genes. Since homologous isotypes have been found both in Xenopus and mammals for each of the three groups of genes, PPAR, RAR, and TR, the Xenopus-mammalian lineage divergence event can be used as a starting time point to determine the speed of evolution up to the present time within each of the three groups. In light of the amino acid sequence differences between the Xenopus and mammalian homologs, it appears that the PPAR genes have evolved 2–3 times faster than the RAR and TR genes. The possible relationship between this relatively rapid evolution and some particularities of PPAR ligand-binding properties will be discussed later.

The chromosomal localization of the PPAR genes has been defined in human and mouse. The human (h) PPAR was mapped on chromosome 22 slightly telomeric to a linkage group of six genes and genetic markers that are located in the general region 22q12-q13.1 (13 ). The hPPAR gene is located on chromosome 3 at position 3p25, close to RARß and TRß, which are at positions 3p24 and 3p21, respectively (14 ). Furthermore, the gene is within 1.5 megabases (Mb) of D3S1263, which is a suitable polymorphic marker that could be used for linkage analysis to evaluate a potential contribution of PPAR to lipid metabolism-related diseases (19 ). Finally, the hPPARß has been assigned to chromosome 6, at position 6p21.1-p21.2 (20 ). In the mouse, PPAR is located on chromosome 6 at position E3-F1, while PPAR and PPARß are found on chromosome 15 and 17, respectively (21 ).

The mouse and hPPAR genes characterized to date reveal a common organization of the translated region in six coding exons with the following distribution: one exon for the N-terminal A/B domain, two exons for the DNA-binding domain (DBD)–one for each of the two zinc fingers–, one exon for the hinge region, and two exons for the ligand-binding domain (LBD). The mouse PPAR gene spans at least 30 kb and comprises a total of eight exons, with two exons corresponding to the 5'-untranslated region and the last exon of the LBD comprising the 3'-untranslated region (22 ). For the PPARß gene, only a partial organization in Xenopus, which corresponds to the six exons of the translated region, has been reported so far (23 ). The human and mouse PPAR genes extend over more than 100 kb of genomic DNA and give rise to three mRNAs, PPAR1, PPAR2, and PPAR3, that differ at their 5'-end as a consequence of alternate promoter usage and splicing (Fig. 1). PPAR1 is encoded by eight exons, comprising two 1-specific exons for the 5'-untranslated region, A1 and A2, and the six coding exons that are common to all three mRNAs. PPAR2 is encoded in seven exons, the first one, exon B, comprising the 2 5'-untranslated region and encoding additional N-terminal amino acids specific of 2. On genomic DNA, this 2-specific exon is located between the second mPPAR1 exon (A2) and the first common exon (19 24 25 ). A third mRNA, PPAR3, encodes the same protein as PPAR1 but is controlled by an alternative promoter located in the region flanking exon A2 in 5' (Fig. 1) (26 ).

View larger version (20K): [in this window] [in a new window]   Figure 1. hPPAR isoforms. The genomic organization of the hPPAR gene is shown. Two PPAR isoforms are produced by the differential use of three promoters and alternative splicing of the three 5'-exons A1, A2, and B1. Exons 1–6 are common to all three transcripts. A scheme of the three mRNAs obtained is drawn, and the size of the proteins obtained is indicated. Note that transcription from the promoters 1 and 3 results in the same protein of 477 amino acids. In the human gene, the splicing event between exon A2 and exon 1 generates an AUG translation initiation codon six nucleotides upstream of the one used in other species, which is located at the very beginning of exon 1. Therefore, the hPPAR1 protein has two additional amino acids at its N terminus compared with the rodent PPAR1 protein. The PPAR2 protein of 505 amino acids is produced by transcription from the promoter 2. The number of additional N-terminal amino acids in PPAR2 vs. PPAR1 is 28 in humans and 30 in rodents.

1. PPAR response elements (PPREs) and PPAR:RXR binding properties. PPRE was first characterized by using synthetic oligonucleotides and was defined as a direct repeat of two core recognition motifs AGGTCA spaced by one nucleotide, thus also called DR1 (28 ). The first natural PPRE, found in the promoter of the acyl-CoA oxidase gene (15 29 ), and all natural PPREs subsequently identified fulfill these DR1 criteria, which allow PPREs to be discriminated from other direct repeat response elements of the TR/RAR class of receptors, such as the one recognized by vitamin D receptor (VDR) (NR1I1) (DR3), TR (DR4), and RAR (DR2, DR5). However, the detailed analysis of the CYP4A6 and malic enzyme genes PPRE, together with a sequence comparison of 19 native PPREs and subsequent mutational analyses, defined additional PPRE determinants (30 31 32 33 ). The three following properties can be added to the initial PPRE definition as a DR1: an extended 5'-half-site, an imperfect core DR1, and an adenine as the spacing nucleotide between the two hexamers, giving the following consensus sequence PPRE: 5'-AACT AGGNCA A AGGTCA-3'. These particularities most likely add discriminating parameters that contribute to PPAR:RXR binding selectivity vs. homo- and heterodimers of other members of the superfamily, some of which also recognize a DR1 type element (see below).

The PPRE structure as an extended direct repeat motif imposes a polarity to the bound heterodimer. PPAR interacts with the upstream extended core hexamer of the DR1, whereas RXR occupies the downstream motif (31 34 ). This represents a reversed polarity as compared with VDR:RXR and TR:RXR bound to DR3 and DR4, respectively, where RXR occupies the upstream core hexamer of the direct repeat. This difference in binding polarity between PPAR:RXR and VDR:RXR or TR:RXR is, at least in part, determined by the 5'-extended half-site in the PPRE. Receptors binding as monomers, such as NGFI-B (NR4A1), ROR (NR1F), and RevErbA, also require an AT-rich 5'-extended binding site (35 ). Interaction of these receptors with the 5'-flank is thought to involve the receptor region immediately C-terminal to the second zinc finger, called carboxy-terminal extension (CTE). While PPARs are unable to bind DNA as monomers, it has been demonstrated that the CTE region of PPARs in PPAR:RXR is indeed responsible for the recognition of the 5'-flank of the DR1 in PPREs (36 ). The inability of PPAR to bind as a monomer has been attributed to the N-terminal region since deletion of the A/B domain of PPAR allowed the truncated receptor to bind to a PPRE as a monomer. Limitation of the DNA binding capacity of PPAR by its A/B domain might reflect evolutionary changes that allow PPAR to functionally diverge from its monomeric cousins (36 ). While the three-dimensional structure of the DBD and CTE region of PPAR has not yet been solved, some of its properties can be inferred from detailed biochemical studies and structural analyses of RAR:RXR and TR:RXR bound to direct repeat sequences (37 38 39 40 41 42 43 44 45 ). Structural and biochemical analyses of RAR:RXR bound to a DR1 element demonstrate that the crucial amino acids for heterodimerization within the 5'-positioned receptor (RAR) are located in the second zinc finger, outside the first knuckle called D box, while the 3'-positioned receptor (RXR) contributes to the dimerization interface via its CTE region. Exchanging the specific PPAR D box, which has only three amino acids instead of five in other members of the superfamily, with that of RXR did not alter PPAR:RXR binding to a PPRE (G. Krey and W. Wahli, unpublished observations), consistent with the exclusion of the D box of the 5'-positioned receptor from the dimerization interface. The recent crystal structure analyses of the Rev-Erb DBD dimer bound on a DR2 (Rev-DR2) further confirm the presence of bonds between the tip of the second zinc finger of the upstream receptor and the GRIP box (VRFGRIPK residues) contained in the CTE region of the downstream receptor (46 ). Interestingly, PPAR, which possesses the same GRIP box as Rev-Erb, is also capable of binding as PPAR:RXR on a DR2 if the spacing sequence between the half-sites corresponds to that found in Rev-DR2 (AGGTCATCAGGTCA) in opposition to an alternative DR2 (AGGTCAGGAGGTCA) to which it does not bind. Furthermore, transcriptional activation by PPAR:RXR can be obtained through the Rev-DR2 that contains the conserved 5'-extended sequence which is recognized by dimers of Rev-ErbA and ROR (47 ). Therefore, a possibility of cross-talk exists between PPAR:RXR and these receptors on 5'-extended DR2 elements (36 ). The polarity of PPAR:RXR on such elements and the functional consequences of the formation of this complex have not yet been evaluated.

The fact that some tissues express more than one PPAR isotype raises the question of PPAR isotype-specific PPRE recognition. Assessment of the relative DNA-binding capabilities of the three PPAR isotypes (, ß, ) to 16 native PPREs led to the classification of PPREs into three functional groups: strong, intermediate, and weak elements, which correlates with the level of the PPRE conformity to the consensus element (32 ). Surprisingly, the number of identical nucleotides in the core DR1 region is rather homogeneous across the different elements, and it is mainly the number of identities of the 5'-flanking nucleotides, rather than that of the stricto-sensu core DR1, which determines the binding strength of a given PPRE. In all cases, PPAR binds more strongly than do PPAR and PPARß and is thus less dependent on a well conserved 5'-flanking extension. In contrast, conservation of the 5'-flank is particularly essential for PPAR binding and therefore contributes to isotype specificity. The PPAR DNA-binding activity is also modulated by the isotype of the RXR heterodimeric partner. Binding of PPAR:RXR to strong elements is reinforced when RXR is the partner, whereas heterodimerization with RXR is more favorable for binding to weak elements. However, it remains to be seen how these in vitro observations translate into selective recognition of the PPREs within their natural genomic and chromosomal environment.

2. Hormonal cross-talk occurring at the level of DNA binding. Direct repeat elements with a 1-bp spacing are also recognized by RAR:RXR, as well as RXR, androgen receptor-related protein-1, hepatocyte nuclear factor 4 (HNF-4) (NR2A), and chicken ovalbumin upstream promoter-transcription factor (NR2F) homodimers (48 49 ). Accordingly, HNF-4 and chicken ovalbumin upstream promoter-transcription factor homodimers can displace PPAR:RXR from its binding site and thus compete with PPAR signaling (50 51 52 53 ). Evaluation of the biological significance of the competition between PPAR and other members of the superfamily for binding to PPRE requires the consideration of at least two parameters. First, the subtle sequence determinants that we described above are important for nuclear receptor discrimination. A recent study shows that single point mutations applied to the core DR1 motif differently affect the binding affinity of HNF-4, androgen receptor-related protein-1, RAR:RXR, and PPAR:RXR (54 ). Thus, the conjunction of a core recognition motif that deviates from the consensus with a specific 5'-flanking sequence, as seen in many natural PPREs, may result in preferential binding of PPAR:RXR (30 31 ). Second, the relative amount of each type and isotype of nuclear receptor within a cell is of great importance in such a cross-talk.

Functional PPREs are almost exclusively represented by DR1-like elements. In addition to binding to the Rev-DR2 dicussed above, another exception is the ability of PPAR:RXR to recognize an estrogen response element (ERE) (55 ). Although an ERE-containing reporter plasmid can be transactivated by PPAR:RXR, no natural ERE-containing gene has been identified that is coactivated by estrogen receptor (ER) (NR3A) and PPAR:RXR. On the contrary, competition for the ERE leads to a PPAR:RXR-dependent repression of the ER-mediated transactivation of the vitellogenin gene A2 promoter as seen in transfection experiments. Thus the possibility of a hormonal cross-talk through an ERE exists (55 56 ), and genes might be found to be coregulated by ER and PPAR:RXR, and consequently by estrogens, fatty acids, and 9-cis-RA, in a natural cell physiological context and in a promoter- and cell type-specific manner.

RXR is a common DNA binding partner to many nuclear receptors of the steroid/thyroid receptor superfamily, including PPAR. Consequently, competition between these receptors for their common partner can occur. Reciprocal negative interactions between the PPAR and TR signaling pathways, through a mechanism of RXR sequestration, was indeed demonstrated in transfection assays (57 58 ). In these experiments, the relative amount of PPARs and TRs, respectively, determined which receptor was dominant, i.e., which signaling pathway inhibited the other. In vivo, such competition is likely to occur only when the amounts of RXR are limiting. Whether the relative amounts of TR, PPARs, and RXR meet these conditions in any tissue in vivo is so far unknown. A similar competition has been proposed to occur among PPAR isotypes (59 ). As a consequence, if several PPAR isotypes are coexpressed in a single cell type and if RXR amounts are limiting, there is a possibility of differential activity of the expressed isotypes.

Interestingly, it was observed that expression of RXR abolished PPAR stimulation of the PRL promoter in pituitary GH4C1 cells (60 ). Analysis of this phenomenon revealed that stimulation of the PRL promoter by PPAR was mediated by protein-protein interaction rather than binding of PPAR:RXR to the promoter. The mechanism proposed is a ligand-dependent association of PPAR with the transcription factor GHF-1, which stimulates transcription and implies that PPAR would act similarly to a coactivator in this specific situation. Overexpression of RXR is thought to titrate out PPAR and therefore suppress its association with GHF-1 and consequently its stimulatory effect.

C. PPAR ligand-binding properties
One of the reasons for the present infatuation for PPARs lies in their particular ligand binding properties, making them attractive therapeutic targets. As we describe below, PPAR moved from the status of orphan receptor to that of generous host, capable of specifically interacting with more than one ligand, including some important natural compounds such as fatty acids. This section will end with information gained from the x-ray crystal structure of the PPAR and PPARß LBD, which provides a link between structural and functional viewpoints.

1. Tools for PPAR ligand identification. The first molecules able to activate PPAR were identified in cultured cells cotransfected with a GRE- or ERE-containing reporter gene together with an expression vector encoding the chimeric receptor GR_DBD-PPAR_LBD or ER_DBD-PPAR_LBD. Compounds that trigger a stimulation of the reporter gene expression when added to the culture medium have been categorized as PPAR activators; the first identified were the fibrate hypolipidemic agents known to induce peroxisome proliferation in rodents, followed soon after by fatty acids (5 10 15 61 ). However, since activation might result from indirect events such as production of a metabolite of the test compound, release of an endogenous ligand, or activation of a cell surface-initiated signaling pathway, these compounds had to be tested further for direct binding to the PPARs.

As could be anticipated from transactivation assays, classical competition assays using radioligands first identified some PPAR and PPAR ligands with a relatively broad structural diversity (62 63 64 65 66 67 ). Additional techniques have then been adapted or developed to allow the screening of a large number of compounds (for review and technical aspects, see Ref. 68 ). The Scintillation Proximity Assay (SPA) is an equilibrium method that uses scintillation to measure the interaction between a molecule prebound to a fluomicrosphere and a radioactive ligand (69 ). It has been recently adapted to the evaluation of PPAR ligands in competition assays (70 ). The Differential Protease Sensitivity assay (DPSA) relies on a ligand-dependent reduction of PPAR sensitivity to enzymatic proteolytic cleavage (71 ). The Ligand Induced Complex (LIC) assay detects ligand-dependent binding of limiting amounts of PPAR:RXR to a PPRE (72 ). Based on the hypothesis that ligand binding to PPAR would induce interaction of the receptor with transcriptional activators, we have developed a novel sensor assay, termed Coactivator-Dependent Receptor Ligand Assay (CARLA) in which we measure the ability of a compound to induce PPAR-SRC1 interaction (73 ). Because of its strong interaction with PPARs, p300/CBP (cAMP response element-binding protein) can also be used in CARLA (74 ). In addition to ligand identification, these assays revealed three peculiarities of PPAR ligand binding properties that have important consequences for PPAR biology. First, in contrast to TR, RAR, VDR, ER, or GR (glucocorticoid receptor) (NR3C1), PPARs accommodate several types of ligand, and the above-mentioned in vitro assays have demonstrated that most of the known PPAR activators are bona fide ligands (75 ). Second, and as a corollary, most of the molecules that specifically bind to PPAR do so with a rather low affinity as compared with the affinity of classical hormones for their cognate receptor. Third, there is some overlap in ligand recognition by the different PPAR isotypes, some ligands binding to more than one isotype although with different affinities. Although the known natural PPAR ligands fit well in our present understanding of PPAR functions, the question remains open whether, in addition, highly selective natural ligands exist with much higher affinity for each of the PPAR isotypes. Below is a presentation of the main PPAR ligands, natural and synthetic, discovered so far.

2. PPAR, PPARß, and PPAR ligands. The identification of unsaturated fatty acids as PPAR ligands (Table 1A) provides firm evidence that at least part of the PPAR-dependent transcriptional activity of fatty acids results from a direct interaction of the nuclear receptor with these molecules. These fatty acids bind all three PPARs, with PPAR exhibiting the highest affinity, at concentrations that are in agreement with their circulating blood levels. In contrast, the very long chain fatty acid, erucic acid (C22:1), which is a weak ligand, appears more selective for hPPARß than for hPPAR and hPPAR, as measured in transfection assays using chimeric GR-PPAR proteins (76 ). Compared with the unsaturated fatty acids, saturated fatty acids are poor PPAR ligands in general (66 72 73 ), whereas phytanic acid, a dietary branched-chain, isoprenoid-derived fatty acid, efficiently binds PPAR (77 ). The discovery that some fatty acids can act as hormones that control the activity of transcription factors demonstrated for the first time that fatty acids are not merely passive energy-providing molecules but are also metabolic regulators. This finding opens novel perspectives for deeper understanding of energy metabolism and therapeutic interventions. Future investigations examining the differential tissue distribution of PPAR isotypes with respect to qualitative and quantitative fatty acid content of each tissue might be very informative for further understanding the specific roles of PPAR isotypes and their fatty acid-mediated activation.

The CARLA as well as the DPSA and the LIC assays have proven effective tools for the identification of interesting synthetic compounds as ligands (Table 1B). These include some hypolipidemic agents such as fibrates, of which clofibrate and the potent Wy-14,643 compound preferentially bind PPAR. Thiazolidinediones (TZDs), which includes troglitazone, pioglitazone, and BRL 49653, now called rosiglitazone, are a class of antidiabetic drugs that are structurally derived from clofibric acid but selectively bind PPAR (62 84 ). This functional association of a key regulator of lipid metabolism and an antidiabetic drug has important implications with respect to the pharmacological use of these compounds, on the one hand, and for the link that it emphasizes between lipid and glucose metabolism on the other. This link is further reinforced by the discovery of a novel series of antihyperglycemic and antihyperlipidemic agents that are PPAR agonists (85 ). Other synthetic compounds that bind to PPARs include the arachidonic acid analog ETYA and some agonists and antagonists of the leukotriene membrane receptors (75 ). Intriguingly, the screenings for natural and synthetic ligands were not very successful in identifying PPARß ligands. Bezafibrate is a Xenopus PPARß-specific ligand (66 73 ), but its activity is much weaker on the mammalian PPARß. In transfection assays, the rat PPARß can be activated by the nonmetabolizable, substituted fatty acid -bromopalmitate. However, nonspecific toxic effects of -bromopalmitate are found at doses close to those required for PPARß activation (S. Basu-Modak, P. Escher, B. Desvergne, and W. Wahli, unpublished data). A novel series of fibrate derivatives, non-TZD compounds, was recently described as human-specific PPARß agonist and will aid in the functional analyses of this elusive PPAR subtype (74 ).

The recent advent of the combinatorial chemistry technology opens new opportunities for the identification of PPAR ligands. Instead of relying on classical large and diverse compound libraries, Brown et al. (86 ) designed a solid phase synthesis of biased chemical libraries of fibrates (so-called focused library) based on the observation that fibrates have activity on the three PPARs. Screening of the library identified a pool of compounds with activity on each of the three PPAR isotypes, of which the compound GW 2433 exhibits a high, although not selective, affinity for xPPARß. Thus, this approach may offer possibilities to develop selective and potent ligands for the three PPAR isotypes and has already provided a source of information about the ligand preferences of the three PPAR isotypes.

3. Species specificity in ligand recognition. Species difference in ligand recognition, already mentioned above for bezafibrate and xPPARß vs. mammalian PPARß, was first investigated with the PPAR isotypes from Xenopus, mouse, and human, which differentially respond to two PPAR ligands, Wy-14,643 and ETYA (67 ). Two amino acid residues in helix 3 of the LBD are responsible for the preferential responsiveness of Xenopus and hPPAR to ETYA and of mouse PPAR to Wy-14,643 (67 ). This identification of structure-function relationships involved in PPAR ligand binding specificity is of interest for drug development and may now be extended to additional compounds. These species differences, which have not been described for other nuclear receptors, raise two issues. The first, of practical importance, is that toxicological tests of PPAR ligands in whole animals must take into account possible species differences. The second is that the species-related ligand binding specificity may be linked to the speed of evolution of the PPAR genes. This might reflect an adaptation of the PPAR signaling pathways to nutritional patterns that can differ from species to species.

4. x-Ray crystal structure of the PPAR LBD. Although the LBD is less well conserved than the DBD between nuclear receptors, structural analyses of this domain performed with liganded RAR and TR LBDs and unliganded RXR LBD have revealed a common structural tridimensional fold, which consists of an antiparallel -helical sandwich of 12 helices (helix 1 to helix 12) organized in three layers with a central hydrophobic pocket. Upon ligand binding, the swinging of helix 12 or activation function helix (HAF) closes the ligand binding pocket like a lid, in a so-called "mouse trap model" (87 88 89 ).

The x-ray crystal structure of the human apo-PPAR LBD and apo-PPARß reveals an overall fold very similar to that of the above mentioned LBDs from helix 3 to the C terminus (90 91 92 ). However, some distinct differences are apparent. The core AF-2 activation domain in the apo-PPARs is folded against the ligand binding pocket in a conformation similar to that observed in the holoforms of PPAR and other nuclear receptors. An additional helix, called helix 2', which is found between the first ß-strand and helix 3, together with a placement of helix 2 that differs from other nuclear receptor tertiary structures, provides an easy access to the hydrophobic pocket for ligands. The region between helix 2' and helix 3, corresponding to the loop in RAR is extended and is the most thermally mobile loop and participates in the structural changes occurring upon ligand binding. The ligand binding cavity is buried in the bottom half of the LBD and is particularly large, 1300 ų, of which the ligand occupies only about 30–40%. It is thus larger and more accessible than in other known LBDs [compare with the cavity in TR 600ų, most of this volume being occupied by the T₃ molecule 530 ų (89 )]. The T-shaped cavity in PPAR comprised one region–the horizontal bar of the T–of 20 Å in length which lies parallel to helix 3; a second cavity region of 16 Å in length is orthogonal to the first and extends to the C-terminal AF2. The main amino acids involved in bonds with rosiglitazone are depicted in Fig. 2. Two histidine residues, H323 and H449, participate in the fixation of the TZD head group and are proposed to permit similar links with -substituted carboxylic acids (91 ). The Y-shaped cavity in PPARß comprises three arms of about 12 Å in length, the left arm being rather polar in character. Eicosapentaenoic acid occupies this pocket in two distinct conformations, with the acid group and eight first carbon units fitting in the left arm oriented toward the AF-2 helix, while the hydrophobic tail either bends upward or downward into the up or bottom arm of the pocket, respectively (90 ). The same network of hydrogen bonds as seen with roziglitazone and PPAR forms between eicosapentaenoic acid and PPARß AF-2 (see Fig. 2). These characteristics also explain that PPARß ligands are preferentially unsaturated fatty acid, given the requirement of a flexible hydrocarbon tail, and have an optimal length, long enough for sufficient stabilizing hydrophobic interactions and short enough for being docked inside the cavity (90 ). In conclusion, these key interactions associated with the relatively free non-specific interactions that the hydrophobic part of ligands can develop within the large cavity explain the promiscuous behavior of PPAR with respect to ligand binding. One consequence might be that different functional activities of a ligand might reflect different binding conformation in the cavity (90 ). Together with the easy access provided by the extra helix 2' and the tertiary placement of helix 2, these characteristics define PPAR as a nuclear receptor that has evolved to bind to multiple natural ligands with relatively low affinity, as reported above (91 ).

View larger version (76K): [in this window] [in a new window]   Figure 2. General structure and LBD of PPARs. A, Scheme indicating the four-domain structure of PPARs, which is the same as for the other members of the nuclear receptor superfamily. Coordinates of the boundaries of each domain are given for the hPPARs. The number inside each domain corresponds to the percentage of amino acid sequence identity of human PPARß and PPAR relative to PPAR. The region whose amino acid sequence is shown in panel B is underlined, and the coordinates are according to the following Genbank accession numbers: PPAR, L02932; PPARß, L05792; PPAR, X90563. DNA indicates DBD; ligand indicates LBD; aa, amino acids. B, Sequence alignment of the human (h), mouse (m), and Xenopus (x) LBD of PPAR (PPARg), PPARß (PPARb), and PPAR (PPARa). The secondary structure adopted by hPPAR is indicated above the sequence in boxes for -helices (H) and arrows for ß-strands (s). Amino acids that are involved in determining the ligand entry site, ligand binding, dimerization, and interaction with SRC-1 are indicated by colored filled boxes, for hPPAR as well as for the other PPARs when conserved. Residues involved in specific interactions with the carbonyl group, the benzene group, and the sulfur atom of rosiglitazone are indicated by a black dot, a triangle, and an asterisk, respectively. These data are derived from the x-ray crystal structure of the LBD of the hPPAR isotype in a complex containing the ligand rosiglitazone (BRL 49653) and 88 amino acids of human SRC-1 (91 ). Results obtained from the crystal structure of the LBD of the hPPARß isotype containing the fatty acid eicosapentaenoic are consistent with those of PPAR (88 ). Individual residues for ligand entry and ligand binding to PPARß are indicated by the corresponding colored empty boxes. The dimerization PPAR:RXR interface is postulated, taking into account the residues conserved in RXR that contribute to the interface of a PPAR:PPAR homodimer observed in crystal solution (91 ). The mutations previously characterized and cited in the text are indicated by vertical lines below the sequence of helix 3, helix 10, and HAF (helix containing the activation function 2 core).

Finally, the crystal structure also revealed coiled-coil interaction between helix 10 from two PPAR molecules forming a homodimer, reinforced by salt bridges involving helices 9 and 10. While PPAR homodimers do not seem to occur in vivo, these observations are similar to those described for RXR homodimers (87 ) and are likely to reflect the contacts involved in PPAR:RXR. Consistent with these observations, a deletion comprising helix 10 and HAF of PPAR impairs heterodimerization with RXR (71 ). Similarly, a leucine to arginine substitution at position 433 (marked by a vertical line in Fig. 2B) in helix 10 of PPAR also abolishes heterodimerization with RXR (58 ).

D. Alternative pathways for PPAR activation
1. PPAR and PPAR are phosphoproteins. Several nuclear hormone receptors, including PPARs, are regulated by phosphorylation in addition to ligand-dependent activation. PPAR was first shown to be a phosphoprotein in primary rat adipocytes in culture. Treatment of these cells with insulin increases PPAR phosphorylation. In parallel, transfection studies in CV-1 cells and HepG2 cells revealed that insulin increases by nearly 2-fold the transcriptional activity of PPAR, as well as that of PPAR (94 ). This insulin effect occurs through the phosphorylation of two microtubule-associated protein (MAP) kinase sites, identified at positions 12 and 21 in the A/B domain of hPPAR (95 ). Using cotransfections in a Chinese hamster ovary cell line that expresses the insulin receptor, Zhang et al. (96 ) demonstrated a synergistic effect between insulin treatment and PPAR ligand-dependent activation on the expression of the target gene aP2. Because this effect was partially inhibited by the addition to the culture medium of a MAP kinase inhibitor, the insulin effect was correlated with PPAR phosphorylation observed in vitro upon exposure to purified MAP kinase (96 ). Contrasting results were obtained when exploring the role of growth factors in modulating PPAR activity (97 98 ). The epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) decrease the transcriptional activity of PPAR while increasing PPAR phosphorylation through MAP kinase signaling. A unique MAP kinase target site, which can be used by both extracellular signal-regulated protein kinase (ERK) and c-Jun N-terminal kinase (JNK) (99 ), was mapped at serine 82 in the N-terminal domain of mPPAR1, which corresponds to serine 112 of mPPAR2. Substitution of this serine by alanine leads to a loss of PDGF-mediated repression of PPAR activity (97 98 ). Comparable MAP kinase-dependent PPAR phosphorylation and inhibition of activity were obtained in 3T3-L1 cells with PGF2, an arachidonic acid derivative that acts through a membrane receptor and has a potent inhibitory effect on adipogenesis (100 ). At the molecular level, the mutant PPAR2 Ser112Asp exhibits a decreased ligand binding affinity and coactivator recruitment. Limited protease digestion of this mutant also results in an altered digestion pattern as compared with that of the native form of PPAR2, when performed in the absence of ligands. These differences suggest that the N terminus, more specifically the phosphorylation status of the serine 112, plays a role in the conformation of the unliganded receptor, thereby regulating the affinity of PPAR for its ligands (101 ).

How the same changes in phosphorylation can lead to an activation or an inhibition of PPAR signaling, depending upon the nature of the triggering signal, insulin or growth factor, respectively, is unclear but likely involves either specific pleiotropic actions or use of different kinase pathways by these hormones acting on metabolic processes. For example, the insulin-mediated up-regulation of phosphoenolpyruvate carboxykinase (PEPCK) gene expression is independent of the Ras/MAP kinase pathway but relies on phosphatidylinositol 3-kinase (102 ). Another example is the inhibition of PPAR activity by GH through the Janus kinase 2-signal transducer and activator of transcription 5b (JAK2-STAT5b) pathway. This inhibition requires a nuclear and transcriptionnally active STAT5b molecule and likely occurs via an indirect mechanism (103 ).

Thus, the remaining open questions concerning PPAR phosphorylation emphasize the importance that posttranslational site-specific modifications may have in the cross-talk between cell membrane signaling and nuclear effectors.

2. Activation of PPAR:RXR by RXR agonists. Another alternative activation pathway of PPAR:RXR occurs through ligand binding to RXR. PPAR forms a permissive heterodimer with RXR, meaning that either partner can regulate the transcriptional activity of the DNA-bound complex by interacting with its cognate ligand, on its own or when both partners are liganded. Indeed, cotransfection studies have shown that both members of the PPAR:RXR complex can mediate a response in the presence of their respective ligand. Furthermore, cotreatment of the cells with both ligands results in an additive effect. Thus, the natural RXR ligand 9-cis-RA as well as synthetic RXR-selective compounds such as LG 1069 and LG 100268 can activate a PPRE-driven reporter gene in a PPAR:RXR-dependent manner (28 61 104 ). Other examples are the liver fatty acid binding protein (L-FABP) gene and the ApoAII gene, known PPAR target genes, which are also responsive to 9-cis-RA (105 106 ). In vivo, this ability of PPAR:RXR to transduce 9-cis-RA signal has been associated with the observation that RXR-selective agonists display antidiabetic activities comparable to those obtained with a TZD, which specifically binds PPAR (107 ). This in vivo action of 9-cis-RA is not PPAR specific since it also activates PPAR-inducible genes (108 ). The physiological relevance in the whole organism of 9-cis-RA pathways has been recently underscored by the identification of two 9-cis-retinol dehydrogenases that might participate in the synthesis in vivo of 9-cis-RA (109 110 ). However, interpretation of the above-mentioned results, obtained after 9-cis-RA stimulation in relation to the potential clinical use of RXR agonists, calls attention to the three following points: 1) in addition to acting through PPAR:RXR and PPAR:RXR, RXR agonists in vivo might also recognize and act via NGFIB:RXR and LXR:RXR permissive heterodimers (111 112 113 ); PPARß:RXR-mediated effects can also not be excluded; 2) in the absence of ligands, RXR forms inactive tetramers and addition of RXR-specific ligands preferentially directs the formation of homodimers rather than heterodimers (114 ). Thus, the formation of heterodimers, and the subsequent signaling, also depends on the presence of ligands for the heterodimerization partner (115 ); 3) RXR homodimers, whose formation is favored by 9-cis-RA, bind and transactivate through response elements corresponding to DR1 (48 116 ). In the context of the PPAR:RXR signaling pathway, how much of the 9-cis-RA response is PPAR dependent and how much is relayed by the formation of RXR homodimers remain to be determined. Such a question is brought up, for example, by the apparent contradictory observations concerning apoCIII gene expression whose down-regulation by fibrates is mediated by PPAR (117 ), whereas the same gene is up-regulated by RXR-specific agonists (118 ). While little RXR homodimers are seen binding on PPREs in vitro, the situation in vivo remains to be explored. The use of a more comprehensive panel of RXR agonists, partial agonists, and antagonists should be used for investigating the complex network built up by RXR within nuclear receptor signaling. This is becoming possible with the recent characterization of the LG 100754 compound, which is a specific RXR:RXR antagonist but RAR:RXR and PPAR:RXR agonist (119 120 ).

In contrast to its role in PPAR:RXR, RXR can only work in subordination when heterodimerized with RAR, i.e., the liganded RXR is transcriptionally active only when RAR is itself liganded (Refs. 121 122 123 and references therein). A similar situation may apply for heterodimers between RXR and either TR or VDR. This would prevent retinoids from activating VDR or TR pathways, in contrast to that mediated by PPAR. It is not yet known whether these different transactivation properties reflect the different polarity with which PPAR:RXR, on the one hand, and RAR:RXR, TR:RXR, and VDR:RXR, on the other, bind to their respective response elements.

E. PPAR-mediated transactivation properties
PPAR-mediated transactivation results from the combination of PPAR:RXR binding to a PPRE and ligand activation of this complex. The conformational change of PPAR triggered by ligand binding or by other activation processes, such as phosphorylation, is believed to generate a conformation with new protein-protein interacting surfaces, that will allow specific contacts with a coactivator(s). Subsequently, this complex transduces regulatory action to the basal transcriptional machinery (see Fig. 3).

View larger version (32K): [in this window] [in a new window]   Figure 3. Model for transcriptional activation by PPARs. 1, Scheme of a PPAR-responsive promoter presented in a linear form with a PPRE, two binding sites for transcription factors (white box), the TATA box, and the transcription start site. The same region organized in a repressive chromatin structure is also shown. A hypothetical PPAR:RXR/corepressor complex, which is not bound to DNA, is activated by a ligand that results in a dissociation of the corepressors from the ligand-activated PPAR:RXR complex. 2, The activated PPAR:RXR complex binds to the PPRE producing a change in chromatin structure indicated by histone H1 release. PPRE-bound PPAR:RXR targets a coactivator-acetyltransferase complex to the promoter. 3, The promoter chromatin at the transcription initiation site region is modified by the coactivator-acetyltransferase complex, which acetylates the histone tails (Ac) thereby producing a transcriptionally competent structure. Acetylation of histones is selectively enriched at the transcription initiation region, involving one to two nucleosomes. 4, Additional transcription factors (e.g., Sp1, NF1) and the basal transcriptional machinery, including the RNA Pol II initiation complex, are recruited to the accessible promoter and transcription is initiated.

While the ligand-mediated activity of PPAR is not affected by the deletion of its A/B domain, a ligand-independent activation function AF1 has been defined within this domain, for both PPAR and PPAR. The PPAR AF1 activity was assayed in transfection analyses using chimeric transcription factors fusing the Gal4DBD with the PPAR1 or PPAR2 A/B domain. While both A/B domains are active, the mPPAR2 N terminus, which possesses an extra 30 N-terminal amino acids, is 5- to 10-fold more potent in transcriptional activation than the mPPAR1 N terminus (124 ). Interestingly, mutation of the MAP kinase site at serine 82/112 described above precludes phosphorylation of this site and increases the activity of Gal4-PPAR AF1 (125 ), suggesting that phosphorylation at serine 82/112 not only affects ligand binding affinity as discussed above (101 ) but also directly regulates the AF1 activity. Similarly, hPPAR2 with a proline-to-glutamine conversion at position 113 (given as position 115 in the original article), when overexpressed in murine fibroblasts, is defective in serine 112 phosphorylation and accelerates differentiation of the cells into adipocytes when compared with wild-type PPAR2 (126 ). Insulin, in contrast, enhances the transcriptional activity of the PPAR AF1 (124 ), as well as induces the activity of the PPAR AF-1 domain (95 ), suggesting that insulin also induces phosphorylation of PPAR via an alternative site or a mechanism that may involve posttranslational modification of an auxiliary factor. No regulated activity of the N-terminal domain of PPARß has been reported so far.

2. PPAR interaction with cofactors. There is strong evidence for the crucial inhibitory or stimulatory role played by molecules that provide a bridge between DNA-bound transcription factors and the transcription initiation machinery (127 128 ). The nuclear receptor corepressor (N-CoR) and the silencing mediator for retinoid and thyroid hormone receptor (SMRT) are corepressor proteins that interact with unliganded nuclear receptors, mediating a repressive signal to the promoter to which the complex binds. Interaction with corepressors requires the CoR box, a structural motif in the N-terminal part of the LBD, which has been described in TR and RAR. PPAR interacts strongly with N-CoR and SMRT in solution but not when bound to a PPRE as a PPAR:RXR complex (34 129 ). In association with the absence of a conserved CoR box in PPARs (34 ), these data provide an explanation for the absence of transcriptional repression by unliganded PPAR via its response element, in contrast to the repression activity of unliganded TR and RAR.

The first described nuclear receptor coactivator, steroid receptor coactivator 1 (SRC-1) (130 ), can interact with the PPAR LBD in solution. This interaction is ligand dependent, and we used this property to develop the CARLA screening assay (73 ). Two PPAR binding domains in SRC-1 have been identified (131 ). Furthermore, the liganded PPAR LBD has been cocrystallized with the region of SRC-1 (aa 623–710) encompassing two LXXLL motifs (where X is any amino acid), characterized as consensus sequence found in nuclear receptor-associated factors (132 ). The x-ray crystal structure shows that in the presence of the ligand rosiglitazone, the two LXXLL motifs of a single SRC1^623–710 molecule interact separately with the AF2 helix (HAF, see Fig. 2B) of each receptor molecule of a dimer, making a stable ternary complex: two PPAR LBDs and one SRC1 molecule (91 ). In this structure, the LXXLL helix is oriented by a conserved glutamic acid of HAF (E471, see Fig. 2B, vertical line) and a conserved lysine in helix 3 (K301) of the LBD, allowing the placement of the LXXLL coactivator motif into the hydrophobic pocket formed by helices 3, 4, 5, and HAF of PPAR (93 ). Importantly, distinct amino acids C-terminal to the core LXXLL motif are required for PPAR activation in response to different ligands (133 ). In agreement with this structure, a site-directed AF2 mutant of PPAR (E471A) that has lost transcriptional capability also fails to interact with SRC-1 or CBP (134 ). Functional assessment of the importance of SRC-1 in PPAR-mediated transactivation comes from the overexpression of the nuclear receptor-interacting domain of SRC-1, which inhibits PPAR-dependent transactivation, whereas overexpression of the full-length SRC-1 potentiates ligand-dependent transcription by PPAR:RXR (34 131 ). In addition, microinjection of SRC-1-directed antibodies inhibits the TZD-dependent activation of a PPRE reporter gene in Rat-1 cells, this inhibition being rescued by the coinjection of a plasmid expressing the full-length SRC-1 (135 ). In contrast, PPAR is still transcriptionally active in SRC-1 knock-out (KO) mice, suggesting that its activity can also be mediated through interaction with other nuclear receptor coactivators (136 ).

Some studies have indicated that liganded PPAR might preferentially directly interact with CBP or the related protein p300 (137 138 139 ). This preference for CBP compared with SRC-1 has been confirmed using fluorescence energy transfer as an approach for quantitating such interactions (134 ). CBP/p300s are very large proteins that are essential for the transactivation function of many transcription factors including nuclear receptors (140 141 142 ), AP-1 (143 ), cAMP response element-binding protein (143 144 ), basic helix-loop-helix factors (145 ), STATs (146 147 148 ), and nuclear factor-B (NF-B) (149 ). Evidence from gene inactivation in mice and from the Rubinstein-Taybi syndrome in humans demonstrates that CBP/p300s are limiting factors (150 ). Thus, they are viewed as integrators of multiple signaling pathways, linking membrane receptor signaling and nuclear receptor activation pathways, as well as being a key limiting factor for which all the above mentioned pathways must compete. In parallel to these functional interferences between distinct pathways, the ability of CBP/p300 to simultaneously make contacts with more than one transcription factor might explain some synergy between these factors (127 151 152 ). Clarifying the specific contacts that PPAR may have with CBP/p300 is thus of great importance for understanding the molecular mechanism of the wide range of PPAR action. The first observations along this line showed that deletion of the 20 C-terminal amino acids of PPAR abolished interaction with CBP, whereas the mutated receptor still binds Wy-14,643, suggesting that like SRC-1, CBP interacts with the activation function helix of PPAR (137 ). However, the LBD of the receptor alone (amino acids 281–468) is not sufficient for a stable ligand-dependent interaction with CBP, which also requires the participation of the T box in the D domain (amino acids 166–179). It is noteworthy that CBP can make functional interactions with SRC-1 through leucine-rich motifs in SRC-1 different from those required for interaction with PPAR. Therefore, it becomes apparent that these motifs serve several functions that are likely to control receptor- and ligand-specific coactivator recruitment as well as the assembly of extended complexes required for the transcriptional induction of receptor target genes.

Schulman et al. (139 ) explored a complex mammalian hybrid system involving fusion proteins to decipher the molecular mechanism of the PPAR:RXR activation by RXR agonists through interaction with CBP. This study shows that the activation of the heterodimer through RXR ligand is independent of the RXR AF2 activation domain but rather involves a conformational change of RXR, which is propagated to the unliganded PPAR moiety and leads to a PPAR ligand-independent interaction of the PPAR AF2 domain with the cofactor CBP. However, the system used does not take into account the role of DNA as triggering some specific constraints to the PPAR:RXR complex. Using the same approach, this "phantom ligand effect" was also previously described for RAR:RXR (153 ). In contrast to PPAR:RXR, RAR:RXR cannot be made transcriptionally active by a RXR ligand alone. Moreover, the RXR AF2 domain is crucial for retinoid signaling since a mouse that bears a deletion mutation of the RXR AF2 domain dies around birth with most of the symptoms corresponding to vitamin A deficiency (154 ). Thus, while the findings described above are of great interest with regard to the PPAR:RXR mechanism of action, more experiments are required to integrate them into a physiological context.

Other PPAR interacting proteins, such as PPAR coactivator 1 (PGC1), PGC2, PPAR-binding protein (PBP)/thyroid hormone receptor-associated protein 220 (TRAP220), receptor-interacting protein 140 (RIP140), and androgen receptor-associated protein 70, have recently been cloned (Table 2). PGC1 is a factor that can interact with PPAR (in a ligand-independent manner), TR and ER (in a ligand-independent manner, but with further reinforcement by thyroid hormone and estrogen, respectively), and RAR (in a ligand-dependent manner) (155 ). This factor is of particular interest because of the strong induction of its expression in muscle and brown fat upon cold exposure. Cotransfection assays indicate a major role of PGC1 in activating a brown fat-specific uncoupling protein 1 (UCP1)-reporter gene in the presence of PPAR, RXR, and a cocktail of ligands (troglitazone, 8-bromo-cAMP, and 9-cis-RA) (155 ). The ubiquitously expressed PPAR binding protein (PBP) (156 ), also cloned as the TR-associated protein TRAP220 (157 ) and VDR interacting protein DRIP230 (158 ), was identified in a double hybrid screen using the PPAR LBD as bait. In a cotransfection assay, its overexpression slightly increases PPAR activation while a truncated form has a potent dominant negative effect (156 ). This suggests that PPAR might also use the large multisubunit coactivator complex (DRIPs or TRAPs), which was shown to be anchored through the ligand-dependent interaction of DRIP230/TRAP220/PBP with VDR (158 ). Interaction of the PPAR LBD with RIP140, initially identified in a breast cancer cell line, is ligand independent (159 ). Interaction with the DNA-bound PPAR:RXR only occurs in the presence of 9-cis-RA and not in the presence of a PPAR ligand (160 ), suggesting that RIP140 interacts with the RXR moiety of the heterodimer rather than with PPAR. In transfection assays, the SRC1-mediated enhancement of PPAR activity is down-regulated by increasing doses of RIP140 expressing plasmid, suggesting that RIP140 acts as competitor and inhibits SRC1 binding to PPAR:RXR (160 ).

In response to these interactions, coactivators and corepressors alter target promoter activities by a mechanism that associates chromatin modification–via their intrinsic histone acetyltransferase or deacetylase activity, respectively, as demonstrated for CBP/p300 (162 163 164 )–and physical contact with the transcription initiation machinery (152 ) (Fig. 3). The fact that coactivators are shared by different nuclear receptors and other transcription factors may indicate that coactivators serve an important role in cell growth and differentiation as contributors to the selection of specific nuclear signaling pathways in a spatio-temporal manner. Assessing which and how a signal or biological situation would trigger preferential interaction of one or the other nuclear factor with PPAR is critical for understanding the integration of PPAR-mediated events in the complexity of the nuclear regulatory network.

	III. Physiological Aspects

Top Abstract I. Introduction II. Molecular Aspects III. Physiological Aspects IV. Conclusions References

 
A. Differential expression of PPAR mRNAs
Information on PPAR expression patterns is a first step in understanding the biological significance of the existence of different PPAR isotypes and isoforms. PPAR isotypes are often coexpressed in tissues that are of ectodermal, mesodermal, or endodermal embryonic origin, with relative levels that vary from one cell type to the other. For the sake of clarity, the expression patterns will be presented separately for each of the three PPAR isotypes in Xenopus, rodents, and human.

1. PPAR expression and regulation. In Xenopus, PPAR is expressed at moderate levels during oogenesis. The maternal transcripts persist in the early embryo up to gastrula stages (15 ). They are then replaced by zygotic transcripts at tail bud stage. In the adult, PPAR is expressed in all tissues that have been tested, i.e., liver, kidney, muscle, testes, and fat body. In mouse and rat, PPAR appears relatively late in development (E13.5) in the tissues where it will be expressed in adulthood. In addition, there is a transient expression of PPAR in the developping central nervous system and during skin maturation (8 165 166 ). In the adult rat, relatively high levels of PPAR mRNA are detected in brown fat, liver, kidney, heart, and the mucosa of stomach and duodenum. Retina, adrenal gland, skeletal muscle, and pancreatic islets also express significant amounts of PPAR mRNA (167 168 ). In the human, its levels in the liver appear lower than in the rodent liver (169 ). In addition, a splice variant lacking exon 6 is found, in addition to the full-length mRNA, in all human liver samples examined (169 ). Because of a shift in the open reading frame, the resulting protein is truncated shortly after the DBD, but no functional analyses of this short form is presently available. Other data available in the human indicate that PPAR is well expressed in heart, kidney, skeletal muscle, and large intestine (170 171 ). In summary, and regardless of the species, the expression of PPAR correlates with high mitochondrial and peroxisomal ß-oxidation activities, as exemplified by its high levels in cardiomyocytes and cells of the kidney proximal tubules, which primarily use fatty acids as an energy source. Another example is the enterocytes at the top of the intestinal villi, which carry the main burden of fatty acid absorption and have a very active peroxisomal ß-oxidation.

In rat liver, PPAR expression is subject to negative and positive regulation by insulin and glucocorticoids, respectively (172 173 ). Accordingly, PPAR mRNA and protein levels cycle in parallel with the circadian rhythm of circulating glucocorticoids. Stress situations or fasting, which induce the levels of plasma glucocorticoids, also result in increased synthesis of PPAR in hepatocytes (174 175 ). In contrast, exposure of primary culture of rat hepatocytes to GH for several days decreases PPAR mRNA levels by 50%. This suppression of PPAR expression may participate in the inhibition of peroxisome proliferator-induced peroxisomal ß-oxidation by GH (176 ). A down-regulation of PPAR gene expression was also observed in chronic alcoholic liver disease in the rat (177 ). Finally, an up-regulation of PPAR by its own ligands, fibrates, or FFA has been found in the FaO rat hepatoma cell line and in rat pancreatic islets, but whether the regulation is transcriptional or posttranscriptional remains to be clarified (178 179 ).

2. PPARß expression and regulation. In Xenopus, PPARß also accumulates early during oogenesis and is expressed in oocytes even at higher levels than PPAR (15 180 ). These maternal transcripts slowly disappear in the early embryo up to gastrula stages to be replaced by zygotic transcripts at the neurula stage. In the adult Xenopus, PPARß expression is ubiquitous, with varying levels in different organs. During rat development, it is already present at relatively high levels in embryonic ectoderm and visceral and parietal endoderm at stage E8.5. The expression shows an important peak in the neural tube between E13.5 and E18.5, and then remains ubiquitous at a lower level throughout the end of development (165 166 ). At the adult stage, PPARß is also abundantly and ubiquitously expressed, often at higher levels than PPAR and PPAR. It also remains the most expressed isotype in the adult nervous system (165 166 181 ). It is only weakly expressed in liver, as compared with other tissues such as lung and kidney (4 8 167 ). Although it is abundant in skeletal and cardiac muscle, PPARß cannot be detected by in situ hybridization in smooth muscle cells of the digestive tract. In testis, its expression is very high in Sertoli cells (167 ). Interestingly, its expression is markedly induced in the uterus at the time of blastocyte implantation and remains abundantly expressed in the decidua at the postimplantation stage (182 ). In humans, PPARß is present at moderate levels in all tissues tested, with a higher expression in the placenta and the large intestine (170 171 ). So far, very little is known about the regulation of the PPARß gene. In the ob1771, 3T3-L1, and 3T3-F442A adipose cell lines as well as in the myoblast cell line C2C12, levels of PPARß transcripts appear to be low in proliferating cells and are induced upon differentiation (9 ).

3. PPAR expression and regulation. In contrast to xPPAR and xPPARß, xPPAR mRNA is not detected during oogenesis in Xenopus except for a short transcript that does not encode a full-length receptor (15 180 ). At adult stage, xPPAR has a relatively restricted expression, with the highest levels found in the fat body and moderate levels found in kidney and liver. Similarly, PPAR has a restricted pattern of expression in adult rodents, white and brown adipose tissues being the major sites of expression (183 ). The intestinal mucosa also express high levels of PPAR in the colon and cecum but less in the small intestine (184 185 186 ). Strikingly, PPAR is abundant in lymphoid tissues such as the spleen (red and white pulp) and Peyer’s patches in the digestive tract (167 168 ). Finally, PPAR is also expressed at low levels in the retina and skeletal muscle. In humans, both PPAR1 and 2 are abundant in adipose tissue and are present at low levels in skeletal muscle. In addition, hPPAR1 is also found in liver and heart (170 187 ). In contrast to rodent, hPPAR is detected neither in spleen nor in circulating T lymphocytes, whereas it is expressed in several transformed human B lymphocyte and myeloid cell lines, as well as in primary bone marrow stromal cells in culture (14 ). Intriguingly, circulating lymphocytes or polymorphonuclear cells express a short 0.65-kb PPAR transcript of unknown function, similar to that found in Xenopus oocytes.

Regulation of the hPPAR gene has been analyzed in vitro as well as in vivo. In vitro exposure of isolated human adipocytes to insulin and corticosteroids synergistically induce PPAR mRNA (187 ). In contrast, PPAR is down-regulated by tumor necrosis factor- (TNF), which triggers dedifferentiation of mature adipocytes in parallel to reducing the expression of adipocyte-specific genes (188 189 ). Since PPAR plays a key role in adipogenesis and is the receptor for insulin-sensitizing drugs, regulation of its expression with respect to nutrition, obesity, and diabetes has been studied. In rodents, which produce a high-fat milk, the suckling-weaning transition of the young corresponds to a dramatic change in dietary fat. PPAR2 is increased in white adipose tissue during suckling and the suckling-weaning transition but rapidly reaches a stable plateau (190 ). In adult rats fed by oral gavage for at least 4 days, PPAR is significantly increased in the adipose tissue of rats receiving high-fat meals, but not in the animals receiving high-carbohydrate food (191 ), whereas 48 h of fasting dramatically reduces the expression of both PPAR isoforms in subcutaneous and visceral adipose tissue (192 ). In mice, PPAR gene expression is also regulated by nutrition and obesity. The expression of both isoforms is down-regulated by fasting and by insulin-deficient diabetes, whereas exposure to high-fat diet increases PPAR expression in adipose tissue of normal mice and induces PPAR2 expression in the liver of obese mice (193 ).

PPAR expression was studied in subcutaneous adipose tissue of 14 lean and 24 obese subjects, revealing that adipose tissue of obese humans has increased expression of PPAR2 mRNA, as well as an increased ratio of PPAR2/1, in proportion to the body mass index (BMI) (187 ). In addition, a low-calorie diet specifically down-regulates the expression of PPAR2 mRNA in adipose tissue of obese humans. Increased PPAR2/ has also been observed in obese rhesus monkeys (194 ). At the same time, a study involving 29 subjects with various degrees of obesity concluded that mRNA levels of PPAR1 in abdominal subcutaneous adipose tissue do not correlate with BMI or fasting insulinemia (Ref. 171 ; see also Ref. 195 ). Together these results suggest that, in humans, PPAR2 but not PPAR1 is involved in the control of adipocyte function. This hypothesis provides a possible molecular mechanism for the alterations in obesity of adipocyte number and function but will require further large-scale studies to be validated. Expression of PPAR has also been studied in muscle tissues and cultures from lean subjects, obese nondiabetic subjects, and patients suffering from type 2 diabetes mellitus. PPAR (1 and 2 not distinguished) was increased in both obese nondiabetic and type 2 diabetes mellitus patients in direct relation to BMI and fasting insulinemia, suggesting that abnormalities of PPAR might be involved in skeletal muscle insulin resistance linked to obesity and diabetes (196 ). In addition to these quantitative analyses of expression levels, mutations in hPPAR have been studied with respect to obesity and diabetes. A polymorphism affecting codon 12 of PPAR2, substituting a proline to an alanine, has been found in several different populations, suggesting that it must be of ancient origin (197 ). This substitution has been associated with decreased receptor activity, lower BMI, and increased insulin sensitivity in a study comprising nonobese subjects (198 ). In contrast, three independent studies could not demonstrate a clear association of this alanine substitution with obesity, fat distribution, or type 2 diabetes mellitus (199 200 ), or with lipoatrophic diabetes (201 ). Finally, the pro12ala mutation was found associated with an increased BMI in two different Caucasian populations (202 ). These contradictions emphasize the difficulty to assess the role of a given mutation in the multifactorial and polygenic disorder of obesity. Another mutation, a proline-to-glutamine substitution at position 113 of PPAR (given as position 115 in the original paper), has been found in 4 of 212 obese subjects vs. none in 237 subjets of normal weight. All four subjects with the mutant allele were markedly obese, with BMI values significantly higher as compared with the mean in the other obese subjects. In in vitro assays, this mutation inhibits the phosphorylation of Ser112, a target of MAP kinase, and increases PPAR activity in an adipogenic test (126 ). Finally, a genome wide screen for type 2 diabetes mellitus conducted in Mexican-American affected sib pairs did not reveal any linkage to the D3S1263 marker, which is within 1.5 Mb from the PPAR gene (203 ), whereas evidence for linkage at 3p24.2-p22, i.e., in the vicinity of RARß and PPAR genes, was found in obese Pima indians (204 ). In conclusion, because obesity and diabetes are multifactorial and polygenic diseases, very large-scale studies and/or linkage analyses will be necessary to ascertain the impact of a given mutation with respect to these phenotypes.

B. PPAR target genes and functions in fatty acid metabolism
The first major insights into PPAR biology came from the demonstration of PPAR-mediated control of liver peroxisomal ß-oxidation (15 29 ) and of the role of PPAR in adipogenesis (183 ). Thus, the search and identification of PPAR target genes (Table 3) have been mainly concentrated on hepatocytes and adipocytes, which both play a key role in systemic lipid metabolism, and indeed indicate that PPARs exert a general regulatory effect on lipid homeostasis. However, many other aspects of PPAR physiological roles, often linked to lipid-specific functions, are currently being unveiled and will be discussed in the following chapters. As an aid in placing PPAR-specific action in a broader physiological context, we propose a short and necessarily simplified summary of the pathways of interest.

View larger version (39K): [in this window] [in a new window]   Figure 4. Lipid transport and utilization from intestinal lumen to liver, adipose, and muscular tissues. A scheme illustrates the main features of the different pathways involved. The absorption and processing of the lipids (triglycerides, TG; cholesterol, Chol; phospholipids, PL; fatty acids, FA) in the intestinal mucosa generate the chylomicrons (CHYL), which circulate in the lymph and are delivered to the blood. FA uptake by muscle cells (energy substrate) and adipocytes (storage in form of TGs) after their release from TG by the extracellular lipoprotein lipase produces chylomicron remnants (CHYL R.), which are cleared from the blood by the hepatocytes. The fatty acid reesterified in the hepatocytes are secreted in form of TGs associated with apolipoproteins (ApoLp) in VLDL particles. LDL particles, which are depleted in FA by the action of lipoprotein lipase but enriched in cholesterol, as well as the HDL particles, which participate in the reverse transfer of cholesterol from peripheral cells to the liver, are taken up by hepatocytes. In energy-demanding situations, lipolysis in adipocytes triggers the release of FFA whose main part is transported in the blood by albumin (FFA.Alb), whereas the hepatocytes produce ketone bodies (KB) as fuel for peripheral tissues (muscle, brain, kidney). This figure is not comprehensive and is meant as an aid to illustrate the connected pathways controlled by PPARs.

1. PPARs in the digestive tract. Triglycerides and phospholipids from the diet are mainly absorbed in the duodenum and jejunum, while cholesterol is mainly absorbed in the ileum. In these intestinal regions, the high PPAR and PPARß expression correlates with the expression of the enterocytic fatty acid binding protein (FABPs) genes, the I-FABP and L-FABP, and of the cellular retinol binding protein genes (205 206 ). A strong positive regulation of the L-FABP gene occurs upon dietary intake of long-chain fatty acids or direct ileal infusion of linoleic acid or -bromopalmitate, which are PPAR and PPARß ligands, whereas I-FABP was unaffected (167 206 207 ). A clofibrate-enriched diet also induces L-FABP gene expression (208 ) and further suggests a relationship between PPAR and FABP expression in the gut. Another putative target gene is that of the fatty acid translocase (FAT) which has been proposed to facilitate the transport of long-chain fatty acids into the enterocyte. It is related to the docking receptor CD36, and its expression pattern and regulation of expression closely resemble that of FABP in the small intestine (209 ). Other genes involved in the transformation of long-chain fatty acids into triglycerides and their incorporation into chylomicrons or VLDL particles, such as those coding for the acyl-CoA synthase (ACS) or for the apolipoproteins, are also PPAR target genes, as will be discussed later. Specific functions for PPAR in the colon, in which the three isotypes are expressed, have yet to be defined. Using an antibody directed against the LBD of PPAR but which cross-reacts with PPAR, Mansen et al. (184 ) showed an increasing gradient of PPAR expression from the crypt to the top of the colon villi, which suggests a role of PPAR in fatty acid absorption. The precise localization of the PPAR isotype, cryptic or at the top of the villi, remains under debate and raises questions about the link between PPAR, cell proliferation, and cell maturation in the intestinal mucosa (see also Section III.F) (185 186 ).

2. PPAR, circulating lipoproteins, and cholesterol metabolism. Cholesterol is an essential component of cell membranes and is the molecule from which steroids are synthesized and which serves as precursor for bile acid synthesis. The main source of cholesterol is the diet. If this supply is insufficient, then cholesterol synthesis is induced mainly in liver cells but also in many other cells. Two transcription factors are currently known to have a strong impact on intracellular cholesterol metabolism: SREBP (1 ) and LXR (3 ). Little is known about the putative role of the different PPAR isotypes with respect to regulation of cholesterol, except that cellular cholesterol levels in preadipocyte influence PPAR expression (see Ref. 274 ). This effect is mediated by adipocyte determination and differentiation factor 1 (ADD1)/SREBP1, which is preferentially involved in fatty acid synthesis, whereas SREBP2 plays a role in intracellular cholesterol metabolism. Such functional interactions between transcription factors suggest interconnected regulations of cholesterol and fatty acid metabolism.

In addition to intracellular cholesterol metabolism, the regulation of circulating cholesterol levels has a high physiopathological relevance since, qualitatively and quantitatively, it is a risk factor for atherosclerosis and its associated diseases. The cholesterol-enriched low density lipoprotein (LDL) particles are formed by release of the triglyceride content of VLDL via the action of lipoprotein lipase (LPL) (see Fig. 4). The released fatty acids are either stored in the adipose tissue or oxidized to generate ATP in different tissues, especially muscle. High density lipoprotein (HDL), in contrast to LDL, is considered as a "good" cholesterol-containing lipoprotein particle as it has a protective effect on atherosclerosis development. Indeed, it is instrumental in removing excess cholesterol from extrahepatic cells and in transporting it to the liver and steroidogenic organs, where it is taken up via the scavenger receptor BI (210 ). The role of PPARs in this general picture is reflected by the therapeutic benefits of fibrates, which are the first efficient lipid-lowering drugs to be used. Fibrate treatment both enhances catabolism of triglyceride-rich particles and reduces VLDL production. Furthermore, it stimulates HDL apolipoprotein expression (211 ). One other important mechanism of the fibrate lipid-lowering effect is believed to be an increased LPL activity through PPAR-mediated activation of LPL gene expression. LDL and HDL blood levels also depend in part on the synthesis, mainly by the liver, of the apolipoproteins required for their assembly. Several of these apolipoproteins are regulated by fibrates via PPARs. Fibrates down-regulate the production of apoCIII (117 ), an atherogenic component of apoB-containing lipoproteins, which inhibits LPL activity and impairs the uptake by the liver of triglyceride-rich lipoproteins. Direct support for these apoCIII effects is provided by transgenic animal studies showing a correlation between liver apoCIII expression and plasma triglyceride levels (212 ). Consequently, down-regulation of hepatic apoCIII production by PPAR appears to be beneficial since lipolysis of VLDL particles is increased and the resulting LDL is efficiently removed from the plasma. In humans, apolipoprotein AI and AII, which are the major HDL apolipoproteins, are up-regulated by PPAR through transcriptional control, while ApoAI expression is down-regulated in rodents. Consistently, a PPRE was identified in the human ApoAI promoter, whereas there is no PPRE in the rat promoter. In the latter, the fibrate-dependent repression is mediated by the nuclear receptor Rev-erb, which binds to a negative element and whose gene is a fibrate target gene (213 ). The PPRE of the human ApoAI and ApoAII promoters can also be occupied by RXR homodimers or by other transcription factors such as ARP1, HNF4, EAR2, or EAR3, resulting in a complex and, so far, unclear pattern of regulation (214 215 216 ). Finally, apolipoprotein B secretion as well as VLDL production are inhibited by peroxisome proliferators by mechanisms not yet characterized (217 ). Importantly, all of the above-mentioned apolipoprotein genes (i.e., apoCIII, apoB, apoAI, and apoAII) and the apoAIV gene were previously shown to be regulated by HNF4 (Ref. 218 and references therein). This raises the general question of interference between PPAR and HNF4 signaling, more specifically as to whether other known HNF4 target genes, such as the human coagulation factors VII, IX, and X (Ref. 218 and references therein) or the liver-enriched transcription factor HNF1 (219 ), are also regulated by PPAR.

One difficulty in interpreting these observations comes from the fact that the various fibrates, although primarily activating PPAR, may also have effects on the two other isotypes, most likely resulting in a broad spectrum of related but distinct activities. Finding better isotype-specific ligands might increase control over biological effects but, paradoxically, by being more selective, these ligands may also reduce the efficiency in treating complex disorders such as dyslipidemia. In this context, it will be of major interest to understand the mechanism by which a PPARß-specific ligand can increase the level of HDL, as reported recently (220 ).

3. Pleiotropic roles of PPAR in the liver.

Regardless of the fate of fatty acids in the liver, two first steps – fatty acid transport across the cell membrane and activation into an acyl-CoA – are required for further processing of the fatty acids. These two steps are facilitated through the induction of a fatty acid transporter protein (FATP) and FAT by ligand-activated PPAR (221 222 ) as well as by the up-regulation at the transcriptional level of the long-chain fatty acid ACS gene (223 ). Formation of fatty acyl-CoA by ACS precedes either their incorporation into triglycerides (the anabolic pathway) or their oxidation (the catabolic pathway) by two major pathways: peroxisomal ß-oxidation and mitochondrial ß-oxidation. For each of these pathways, the expression of some key enzymes is up-regulated by PPAR.

a. PPAR and peroxisomal ß-oxidation: Peroxisome proliferation, which can be triggered in rodents but not in humans, corresponds to an increase in the volume density of peroxisomes and of the peroxisomal fatty acid ß-oxidation activity. This activity is inducible by signals such as exposure to cold, high-fat diet, and thyroid hormone, but also by a wide variety of compounds collectively called peroxisome proliferators that includes certain hypolipidemic drugs (224 ). As mentioned above, the name of these receptors is derived from the first discovered PPAR activators, which belonged to this class of compounds (5 ). The fact that PPAR KO mice cease to exhibit peroxisome proliferation upon exposure to the classic peroxisome proliferators, clofibrate and Wy-14,643, demonstrate that PPAR is indeed the main mediator of the pleiotropic actions of this class of compounds (225 ). Accordingly, the first PPAR target genes that have been characterized encode peroxisomal enzymes, more specifically the enzymes of the ß-oxidation pathway. The genes encoding acyl-CoA oxidase (ACO), which is the rate-limiting enzyme in the pathway, enoyl-CoA hydratase/dehydrogenase multifunctional enzyme (HD), and keto-acyl-CoA thiolase are direct targets of PPAR (15 29 226 227 ). In contrast, neither the catalase gene nor the urate oxidase gene, which control the disposal of the H₂O₂ produced by fatty acid oxidation, appears to be directly regulated by PPAR. Peroxisomes also contain enzymes participating in cholesterol and dolichol synthesis, and in the oxidative degradation of polyamines, purines, and D-amino acids. A direct role of PPAR in these pathways has not been reported.

What is the physiological role of PPAR as a mediator of peroxisomal proliferation? With respect to energy homeostasis, peroxisomal ß-oxidation is not directly coupled to an electron transport chain and oxidative phosphorylation. Therefore, the energy released in the first oxidation step (H₂O₂ production) is lost as heat, and the energy released in the second step is conserved in the form of the high-energy level electrons of NADH (228 ). Peroxisomal ß-oxidation appears to be mainly a chain-shortening mechanism of the very long-chain fatty acids (>C20), which predominantly come from the diet and are prevented from entering mitochondria. After a few rounds of peroxisomal ß-oxidation, which removes two carbons at each round in the form of an acetyl-CoA molecule, the shortened chain can then be further degraded to completion in the mitochondrion. The acetyl units (2-carbons), which are generated by the peroxisomal pathway, can be converted to acetylcarnitine, acetate, and acetoacetyl-CoA. Alternatively, they can be used by the fatty acid chain elongation system or serve other biosynthetic purposes in the cytosol (e.g., sterol synthesis) illustrating the substantial role of peroxisomes in fatty acid recycling (228 229 ). Moreover, an isotopomer analysis in HepG2 cells demonstrated that peroxisomal fatty acid chain shortening induced by a PPAR ligand (troglitazone) might also be important for the shortening of saturated fatty acids and might contribute to membrane lipid synthesis (230 ). Finally, peroxisomal ß-oxidation also oxidizes other substrates, such as some eicosanoids and xenobiotics, which are then excreted in the urine as metabolites (228 ). Thus, PPAR, by stimulating peroxisomal ß-oxidation, on the one hand, helps in furnishing fatty acid substrates that can enter the mitochondrion or be used in membrane synthesis and, on the other hand, contributes to the detoxification of endogenous and exogenous active molecules, some of which may be PPAR ligands.

b. PPAR and mitochondrial ß-oxidation: Mitochondrial ß-oxidation greatly contributes to energy production via oxidative phosphorylation generating ATP. The role of PPAR in energy homeostasis is linked to the extent with which PPAR regulates this pathway. As far as energy conservation is concerned, mitochondrial ß-oxidation is approximately twice as efficient as peroxisomal ß-oxidation. The first limiting step in mitochondrial ß-oxidation is the entry flux of fatty acids into the mitochondria, which is controlled by a carnitine-dependent facilitated transport system. This control is not only quantitative but also qualitative since it excludes the very-long-chain fatty acids (C > 20). One of its critical components, the carnitine palmitoyl transferase I (CPT I), catalyzes the formation of fatty acyl carnitine for translocation across the inner mitochondrial membrane. This enzyme is strongly induced by peroxisome proliferators and fatty acids (231 232 ), and a functional PPRE has been characterized in the promoter sequence of the muscle-type CPT I gene (233 234 235 ). PPAR further regulates the mitochondrial ß-oxidative spiral by modulating the expression of the medium-chain acyl-CoA dehydrogenase (MCAD) gene (236 ).

The acetyl-CoA unit produced at each cycle of fatty acid ß-oxidation in mitochondria has three possible fates (Fig. 5): 1) acetyl-CoA condenses with oxaloacetate, normally provided by the glycolytic pathway via pyruvate, to form citrate, which can enter the citric acid cycle for complete oxidation to CO₂ and ATP generation; 2) alternatively, the citrate resulting from the condensation of acetyl-CoA with oxaloacetate is exported into the cytosol for the synthesis of fatty acids or other purposes; 3) if oxaloacetate is low or unavailable because of its use in gluconeogenesis, as seen during fasting or in diabetes, a major portion of the acetyl-CoA is converted to ketone bodies, mainly acetoacetate and 3-hydroxybutyrate. These molecules serve as important energetic substrates for extrahepatic tissues such as skeletal muscle, heart, kidney cortex, and the brain for which it is the only non-glucose-derived source of energy. The mitochondrial hydroxymethylglutaryl-CoA synthase (mHMG-CoAS) is the main enzyme involved in ketone body formation and is directly controlled by PPAR (237 ). Surprisingly, the mHMG-CoAS protein can interact with PPAR in vitro via a LXXLL motif also used by coactivators for interaction with the receptors (see Section II.E.2). In vivo, in the presence of PPAR, mHMG-CoAS is translocated into the nucleus and potentiates PPAR-dependent transcription activation of the mHMG-CoAS gene specifically via the HMG-CoAS PPRE. These interesting findings reveal a novel mechanism whereby the product of a PPAR target gene, which functions as a ketogenic enzyme in mitochondria, also specifically autoregulate its own nuclear transcription by modulating the activity of PPAR (238 ).

View larger version (36K): [in this window] [in a new window]   Figure 5. Fatty acids and energy metabolism. The main pathways that lead to the utilization of fatty acids as energetic substrates and are targets of PPAR action are depicted. The three "devenirs" of acetyl-CoA units are marked by circled 1, 2, and 3. Attention was particularly given to enzymes cited in the text. ACC, Acetyl CoA carboxylase; G6P, glucose 6 phosphate; PPP, pentose phosphate pathway; TG, triglycerides; PL, phospholipids; ß ox, ß oxidation; CPT, carnitine palmitoyl transferase.

c. PPAR and adaptation to fasting and stress: Fasting and stress represent typical situations in which coordination in the liver of PPAR expression and its activation results in an enhanced breakdown of fatty acid into energy-rich units. In such situations, lipolytic stimulation in the adipose tissue increases the plasma levels of nonesterified fatty acids, whose rapid uptake by the liver increases the intracellular concentration of these PPAR activators. Concurrently, PPAR expression is directly stimulated by elevated circulating glucocorticoid levels (173 174 ), while levels of insulin, which counteracts many effects of glucocorticoids, are decreased (172 ). Fasting is also associated with a rapid depletion of the glycogen stores and an increased rate of gluconeogenesis. PPAR null mice have low glycogen stores and, upon fasting, they exhibit a severe hypoglycemia and hypothermia (240 ). These manifestations are accompanied by an enhanced lipid accumulation in the liver and no increase in ketone body production, suggesting a dramatic impairment of fatty acid oxidation (240 241 ). Another link between PPAR and gluconeogenesis is revealed by treatment of wild-type and KO mice with etomoxir, an agent that blocks CPT I activity. This treatment provokes a lethal hypoglycemia in the PPAR KO mice. In contrast, wild-type animals tolerate etomoxir treatment and respond by a strong up-regulation of known PPAR target genes (ACO, CYP4A1, CYP4A3, and MCAD). The hypothesis, consistent with the above-mentioned observations, is that PPAR KO mice suffer from both a depletion of glycogen stores and diminished gluconeogenesis, due to a low acetyl-CoA/long-chain acyl-CoA ratio that inhibits pyruvate carboxylase, a rate-limiting enzyme in hepatic gluconeogenesis. In concert, there is a marked triglyceride accumulation in liver and heart of the dead mice. Another key regulatory enzyme of gluconeogenesis in the liver is PEPCK. The promoter of its gene contains a functional PPRE (242 ) and therefore might be stimulated by PPAR in liver. However, this response element is located in the distal enhancer region of the promoter, which has been shown to be involved mainly in adipocyte-regulated PEPCK expression, and there is no alteration of the PEPCK gene expression in the liver in PPAR KO mice (240 ).

Intriguingly, survival of PPAR KO mice treated with etomoxir presents a strong sexual dimorphism as 75% of PPAR KO females or estradiol-pretreated males survive vs. no survival of PPAR KO males. The pathway and mechanism of this estrogen-dependent rescue are not yet understood (243 ). A remarkable sexual dimorphism of the PPAR KO mice was also observed when they were identified as a model of monogenic, late-onset obesity (244 ). Females develop a more pronounced obesity than males which, in turn, present a marked steatosis in liver associated with the delayed occurrence of obesity. These observations link PPAR with a sexual dimorphic control of circulating lipids, fat turnover, and obesity. Another sexual dimorphism that affects the activity of PPAR is the estrogen-induced peroxisome proliferation in the duck uropygial gland, the function of which is to produce 3-hydroxy fatty acid esters that serve as female pheromones during the mating season (245 ). Expression of PPAR1 is high in this organ, and estrogen induces the formation of a PGD2 metabolite similar to ¹²-PGJ2, able to activate the receptor. These findings raise the possibility that in this tissue, PPAR might be responsible for peroxisome proliferation. While the extent of sexual dimorphism, particularly that of the PPAR KO mice, might depend on the genetic background or other poorly controlled parameters, these observations certainly invite further investigations of the cross-talk or interference between ER and PPAR signaling.

d. PPAR and liver fatty acid synthesis: The idea of PPAR being involved in fatty acid synthesis stems from the demonstration that the lipogenic malic enzyme gene is up-regulated by peroxisome proliferators via PPAR through a well-characterized PPRE (31 246 ). Moreover, basal expression of this gene in the liver is lowered in PPAR KO mice (239 ). The reaction catalyzed by malic enzyme consists of the oxidative decarboxylation of cytosolic malate, which generates pyruvate and leads to the formation of NADPH, required for lipid synthesis (Fig. 5). However, the role of PPAR with respect to fatty acid synthesis in the liver appears complex since other important lipogenic genes are down-regulated by polyunsaturated fatty acids (PUFAs) and insensitive to other PPAR activators (247 248 ). This raises two questions. 1) Does the up-regulation of the malic enzyme gene truly indicate a role of PPAR in fatty acid synthesis?; and 2) Are the regulatory events triggered by PUFAs mediated by PPAR? In an attempt to answer the first question, three hypotheses can be discussed. The activation of both catabolism and anabolism of the same molecules, e.g., fatty acids, would correspond to a futile cycle wasting energy and generating heat. This cycle cannot be much used since malonyl-CoA, which is the first and committed product in fatty acid synthesis, is an inhibitor of the CPT I enzyme, thereby inhibiting mitochondrial fatty acid oxidation (Fig. 5). Thus, from this network of regulation, it appears unlikely that the PPAR-mediated activation of the malic enzyme gene in the liver is dedicated to lipid synthesis. Alternatively, NADPH is not only required for fatty acid synthesis but is also involved in many reductive biosynthetic pathways. A first example is that of the liver cytosolic enzyme stearoyl-CoA desaturase 1 (SCD-1), which catalyzes ⁹-cis desaturation of saturated fatty acyl-CoA substrates and requires NADPH as the second substrate for oxidation. This desaturation increases the ratio of oleic acid vs. stearic acid in membrane phospholipids and thus modifies the membrane fluidity. It has also been associated with a facilitation of fatty acid incorporation into VLDL particles. This gene is itself up-regulated by PPAR and peroxisome proliferators (249 ). Other examples of NADPH-dependent reactions are those catalyzed by mixed-function oxygenases (cytochrome oxidases). Whereas much of the NADPH required for these pathways is provided through the pentose phosphate pathway, the transfer of reducing equivalents from the mitochondria to the cytosol, by means of the malic enzyme shuttle, was previously suggested as being important for monooxygenase functions (250 ). This is consistent with the role of PPAR and fibrates in inducing some of these monooxygenase activities (see below). Finally, one might consider that the activation of the malic enzyme gene increases the amount of pyruvate, which is one major metabolic junction linking glucose, amino acids, and lipid metabolism (Fig. 5).

The second question concerns the mechanisms of action of PUFAs. In most cases, up-regulation of PUFA target genes has been convincingly demonstrated as occurring through PPAR and is reproduced when using characterized PPAR ligands other than PUFAs. In contrast, the PUFA-dependent down-regulation of the Spot14 gene (whose function is unknown) as well as that of the fatty acid synthase (FAS) gene are not observed when using these other PPAR ligands, suggesting the existence of an alternative mechanism of action for PUFAs (248 ). The SCD-1 gene mentioned above also is up-regulated by fibrates but down-regulated by PUFAs (249 ). The malic enzyme gene itself is down-regulated by high-fat diet, although the molecular mechanism has not yet been elucidated. The existence of distinct pathways for PUFA/PPAR-dependent up-regulation and PUFA-induced down-regulation of target genes is further substantiated in PPAR KO mice where PUFAs cannot up-regulate the PPAR target gene ACO as expected, whereas they continue to down-regulate FAS and Spot14 gene expression, demonstrating that this down-regulation is PPAR independent (251 ). The involvement of the nuclear receptor HNF-4 in this PUFA-induced down-regulation has been proposed and is supported by the fact that competition occurs between PPAR and HNF-4 for binding to the same response element, as previously mentioned. Moreover, fatty acyl-CoA ligands can modulate the activity of HNF-4 by either activating or suppressing its action as transcription factor, depending on the chain length and degree of saturation (252 ). Thus, it is possible that HNF-4 is the factor mediating the PUFA-dependent down-regulation of lipogenic enzymes.

e. PPAR and microsomal -oxidation: The cytochrome monoxygenase system plays a central role in the oxidation of a wide variety of endogenous as well as exogenous compounds. The CYP4A enzymes participate in this system as a distinct group of the cytochrome P450 superfamily. They catalyze the -hydroxylation of fatty acids and eicosanoids and are induced by fibrates and other peroxisome proliferators in liver and kidney. -Hydroxylation is, for example, the first step in the neutralization of LTB4, a PPAR ligand, which is then completely degraded through ß-oxidation in the peroxisomes (253 ). At least two of the CYP4A genes, CYP4A1 and CYP4A6, contain a functional PPRE in their promoter sequence and indeed respond in vivo and in cell culture to PPAR activators (23 254 255 ). By comparing PPAR-deficient and wild-type mice, it was shown that insulin-dependent diabetes and starvation result in a strong induction of the hepatic CYP4A genes and other lipid-metabolizing enzymes, such as ACO, through activation of PPAR (256 ). Three main conclusions can be drawn from all these observations: 1) PPAR can provide a negative feedback on the intracellular levels of an endogenous ligand (65 ); 2) a pathophysiological state can induce cellular changes that lead to the activation of PPAR (256 ); and 3) PPAR may have an important role in the detoxification of some xenobiotics. A better assessment of these PPAR detoxification functions is of great importance in the perspective of pharmacological and therapeutic applications.

4. PPAR and adipogenesis.

a. PPAR and the adipocyte differentiation program: The first striking specific characteristic of PPAR, when it was discovered, was its high expression levels in adipose tissue (15 ). A direct role of PPAR in adipogenesis was then suggested by the fact that whereas preadipocyte cell lines, including 3T3-L1 and 3T3 F442A cells, express only trace amounts of PPAR, the appearance of PPAR during adipocyte differentiation precedes that of several markers of late differentiation, such as aP2, PEPCK, and CAAT/enhancer binding protein (C/EBP) (183 257 ). Moreover, PPAR activators such as Wy-14,643, ETYA, and TZDs, were able to promote the conversion of preadipocytes into adipocytes (62 258 259 260 ). Additional strong evidence for the adipogenic role of PPAR came from its forced expression in NIH-3T3 fibroblasts which, in the presence of various activators (ETYA, clofibrate, LY 171883, and linolenic acid), underwent adipose differentiation and accumulated lipids (257 ). A similar adipogenic effect of PPAR was observed in several fibroblastic cell lines (NIH-3T3, BALB/c-3T3, Swiss-3T3), whereas high PPAR expression is found in lipid-laden lung fibroblasts (261 ).

The C/EBP family of transcription factors is also involved in the control of the adipocyte differentiation program (262 ). The expression of the three C/EBP isotypes, , ß, and , follows a specific pattern along the adipose differentiation process. A first transient increase of C/EBPß and expression is followed by the onset of C/EBP expression during the late phase. In this cascade, the appearance of PPAR seems to intercalate between the C/EBPß/ and C/EBP waves (183 263 ). Indeed, C/EBPß expression together with dexamethasone treatment lead to induced PPAR expression and, consistently, C/EBP binding sites have been found in the PPAR2 promoter sequence (264 ). At the time point of strong PPAR expression in differentiating cells, addition of a PPAR activator triggers lipid accumulation (263 ) as well as C/EBP expression, both PPAR and C/EBP being required for establishment of insulin-sensitive glucose transport (265 ). Further evidence for the involvement of C/EBPß in early steps of this cascade comes from a recent study of the TLS-CHOP oncoprotein, which is a fusion translocation liposarcoma-C/EBP homologous protein, found specifically in a malignant tumor of adipose tissue. TLS-CHOP forms heterodimers that cannot bind DNA with C/EBPß. Therefore, C/EBPß function is inhibited and adipose differentiation is blocked, but overexpression of PPAR2 can overcome this blockage in tumor cells (266 ). In agreement with the role of C/EBP as end point in the adipogenic program, C/EBP KO mice have severe reduction of brown fat and white fat mass (267 ). However, a single linear cascade of events is not sufficient to explain adipogenesis since mice that are null allele mutants for both C/EBPß and C/EBP exhibit an apparent normal expression of C/EBP and PPAR but have impaired adipogenesis (268 ). Inhibition of all three C/EBPs by overexpression of a dominant-negative protein A-ZIP/F-1 also results in normal PPAR expression but no fat development (269 ). Conversely, optimal differentiation requires a combination of factors as seen, for example, during the adipocyte conversion of 3T3 fibroblasts in which C/EBPß, C/EBP, and dexamethasone are necessary to induce PPAR expression. A sustained expression of C/EBPß seems also to be important for full PPAR activity (270 ).

Transcription factors involved in adipogenesis also comprise the sterol regulatory element binding proteins (SREBPs). SREBP-1a and -1c are not absolutely required for fatty acid synthesis and adipogenesis but rather act as auxiliary regulators (1 ). SREBP-1c, first identified as an adipocyte differentiation and determination factor called ADD1 (271 ), is induced early in adipose differentiation of mouse 3T3-L1 preadipocytes. Since conditioned medium from cells transfected with ADD1/SREBP contains an unidentified activator(s) for PPAR (272 ), terminal adipocyte differentiation may result from an SREBP-induced production of this ligand, possibly a lipid. In contrast, overexpression of the nuclear form of SREBP1c in the adipose tissue of transgenic mice lowers PPAR expression as well as that of other markers of adipocyte differentiation, generating a model of fat-deprived mice (273 ). This apparent paradox might reflect the importance of the timely schedule of SREBP1c gene activation in the adipocyte differentiation program. The other SREBP isotype, SREBP-2, is essential in regulating cholesterol metabolism. A response element to which SREBP binds has been characterized in the PPAR3 promoter, and cholesterol depletion stimulated this promoter (274 ). This induction of PPAR expression by cholesterol depletion may explain why agents reducing circulating cholesterol, such as the HMG-CoA reductase inhibitors, also lower triglyceride levels.

Growth factors and insulin also regulate adipogenesis; PDGF, EGF, and fibroblast growth factor (FGF) inhibit adipocyte conversion and, as already mentioned (Sections II.D.1 and II.E.1), this effect correlates with a phosphorylation of serine 82 and 112 in the N-terminal domain of PPAR1 and PPAR2, respectively, and with an inhibition of PPAR transcriptional activity (97 98 ). In contrast, insulin and insulin-like growth factor induce adipocyte differentiation and enhance transcriptional activity of PPAR via a mechanism that needs to be elucidated. Werman et al. (124 ) speculate about the respective roles of the ligand-independent and ligand-dependent PPAR domains (AF-1 vs. AF-2) under varying physiological and metabolic conditions to which adipocytes or cells expressing PPAR are exposed. In the presence of high amounts of PPAR ligand, adipocyte differentiation would be favored by the PPAR-induced growth arrest and stimulation of specific adipocyte genes via the ligand-dependent AF-2 domain. When ligand is rare, growth factor-controlled PPAR activity would regulate genes needed for basal adipocyte homeostasis via the ligand-independent AF-1 domain of the receptor.

In addition to PPAR, PPARß is also expressed at significant but lower levels in adipocytes. In ob1771 cells, its expression is activated shortly before the cells reach confluence and start to undergo phenotypic changes linked to differentiation. PPARß was thus proposed as being an early initiator of the differentiation program (9 ). To compare the adipogenic potential of the three PPAR isotypes, their forced expression in fibroblasts followed by exposure to their respective ligands was performed. PPAR has the best adipogenic impact and was the only isotype to be able to cooperate with C/EBP. PPAR also was able to trigger a certain level of adipogenesis while, in this assay, PPARß on its own was inefficient (275 ). However, forced expression and activation of PPARß raise PPAR expression, which, together with the addition of PPAR ligand, leads to adipocyte differentiation (276 ).

During development, adipocytes originate from mesodermal pluripotent cells. Depending on the developmental stimuli, these progenitor cells differentiate into either myotubes, chondrocytes, or adipocytes. In vitro conditions for differentiation of embryonic stem cells into adipocytes are close to those for myotube differentiation, and a mixed culture of adipocytes and myocytes is obtained (277 ). Although in a single given cell these differentiation pathways are mutually exclusive, some degree of plasticity remains. For example, the forced and simultaneous expression of PPAR and C/EBP in G8 myoblastic cells and treatment with PPAR activators inhibits myotube formation, represses the expression of several muscle-specific markers and triggers differentiation into adipocytes (278 ). This transdifferentiation also occurs in nontransfected C2C12 myoblasts exposed to the potent PPAR agonist rosiglitazone 2 days before the culture reaches confluence (279 ). It is tempting to correlate this myotube transdifferentiation obtained in culture with the adipose tissue formation that occurs within muscle in certain pathologies such as in obesity (280 ). Finally, bone marrow stromal cells can differentiate in vitro into adipocytes when treated by TZD (281 ), which correlates with the inappropriate adipogenesis that might occur in bone marrow of dogs treated with TZD (282 ).

b. PPAR target genes in adipose tissue: The phenotypical conversion of fibroblasts or stem cells into lipid-accumulating cells is accompanied by the induction of several specific adipose markers. A direct role of PPAR in the up-regulation of many of the corresponding genes has been described. They encode enzymes involved in fatty acid release such as LPL (283 ), which is secreted by adipocytes and triggers the release of fatty acids from lipoprotein-bound triglycerides in the extracellular space. As in hepatocytes or in enterocytes, the incorporation of long-chain fatty acids into adipocytes might be facilitated by putative transport proteins, FAT and FATP, which are up-regulated by PPAR and - activators (222 284 ). The adipocyte fatty acid-binding protein aP2 gene, as well as the acyl-CoA synthase gene, contains a functional PPRE within its promoter sequence and can be stimulated in differentiated adipocytes (183 223 ). Fatty acid and triglyceride syntheses are also promoted by PPAR-mediated activation of the malic enzyme gene (31 246 ), which is clearly lipogenic in this context, and that of the PEPCK gene (242 285 ) involved in the production of glycerol for storage of the fatty acids in the form of triglycerides. Finally, Wu et al. (286 ) demonstrated that the expression of the gene encoding the insulin-dependent glucose transporter GLUT4, which plays an important role in maintaining glucose homeostasis, is up-regulated by PPAR and TZD. The c-Cbl-associated protein (CAP) also belongs to the insulin-signaling pathway, potentiating the insulin-mediated phosphorylation of the c-Cbl protooncogene. It is only present in mature adipocytes, and its expression is induced upon TZD treatment (287 288 ).

These effects of PPAR in adipose tissue are proposed to be the main mechanism by which TZD improves insulin sensitivity in patients with insulin resistance syndrome. This syndrome is functionally characterized by a poor cellular utilization of glucose, resulting in hyperglycemia, despite an elevated insulin blood level. Obesity and hyperlipidemia are always associated with this syndrome. In such patients, TZDs were shown to be efficient hypolipidemic as well as hypoglycemic agents. A likely mechanism is that increased fatty acid uptake and triglyceride clearance by the adipose tissue redirect fatty acids from the muscle to adipose tissue and thus relieves the fatty acid-mediated inhibition of glucose utilization by muscle cells (reviewed in Ref. 289 ). PPAR may also directly affect the insulin signaling pathway in adipose tissue, as suggested by the TZD-mediated induction of CAP. It is also possible that PPAR in the adipose tissue stimulates the production and secretion of molecules that are regulators of the insulin-signaling pathway in muscle and liver. However, as we will discuss below, the insulin resistance syndrome is also improved by TZD in animals lacking adipose tissue, suggesting that an additional mechanism(s) must be involved.

c. Brown fat vs. white fat: Brown fat, a remarkable heat producer, is best known for affecting the basal metabolic rate, giving protection against cold and regulating energy balance. In agreement with this role, a transgenic mouse in which brown adipose tissue is functionally deleted by a targeted expression of diphtheria toxin A (DTA) has a lower metabolic rate and becomes obese and insulin resistant (290 ). One characteristic of differentiated brown fat cells is the specific expression of mitochondrial UCP1, which uncouples fuel combustion and ATP synthesis by dissipating the mitochondrial proton (H+) gradient generated by the respiratory chain, producing heat instead of ATP (291 ). In humans, brown adipose tissue diminishes and/or is dispersed shortly after birth and the role of the human UCP1 gene remains to be defined. However, two other genes related to UCP1 have been described in rodents and humans: UCP2 which is widely expressed (292 ) and UCP3 which is mainly present in muscle cells (293 294 ). Several lines of evidence support the hypothesis that they also play an important role in BMR: 1) identity with UCP1 of 59% and 57%, respectively; 2) down-regulation when access to food is restricted; 3) a correlation between the levels of induced expression and ability of different strains of mice to cope with high-fat diet while remaining lean; and 4) an uncoupling activity demonstrated in yeast or in transfected cells (291 ). However, up-regulation of UCP2 and UCP3 by total food deprivation may indicate a more complex role, yet to be clarified (295 ). UCP1-deficient mice obtained by targeted inactivation of the gene are sensitive to cold due to a loss of thermoregulation but, surprisingly, are neither hyperphagic nor obese. This later phenotype may be due to compensation by UCP2, which is ubiquitously expressed and induced in the brown fat of UCP1-deficient mice (296 ). In view of UCP functions in energy homeostasis, it is legitimate to ask whether PPARs are directly involved in their regulation. A partial answer will be given below.

As for the white adipose tissue, activation of PPAR is capable of inducing brown adipocyte differentiation from precursor cells (297 ). Whereas the main regulators of the UCP1 expression are thyroid hormones, ß-adrenergic stimulation, and overfeeding, PPAR-mediated regulation of the UCP1 gene also has been demonstrated using differentiating HIB-1B preadipocytes (298 ) and in vivo (299 ). PPAR activation also can induce UCP2 expression in mice liver, but not in BAT, and regulate UCP3 expression in neonatal muscle (300 ). Interestingly, Puigserver et al. (155 ) showed that the expression of the coactivator PGC1, which can interact with PPAR but also TR, RAR, and ER, is strongly induced in brown fat during cold exposure. While the data presented in this latter report do not support an important role of the TZD ligand in activating UCP1 through PPAR/PGC1 interaction, one must not overlook the fact that brown adipose tissue is also characterized by a high level of expression of PPAR, which correlates with high levels of fatty acid oxidation in this tissue. Mice with impaired fatty acid oxidation, through spontaneous and induced mutations in the long-chain and short-chain acyl-CoA dehydrogenase (LCAD and SCAD) genes, are cold sensitive (301 ). Consistently, PPAR KO mice exhibit a marked decreased body temperature when subjected to fasting (240 ). Thus, if PPAR-mediated fatty acid oxidation is the pathway preferentially targeted by PGC1, this feature might provide an explanation for the rather poor activity of TZD. Brown adipose tissue is likely to represent a remarkable tool for exploring how PPAR and PPAR target different genes, and possibly opposite pathways in the same cell population.

d. The adipose tissue as an endocrine tissue linked to the systemic hormonal network: Renewed interest in adipose tissue functions arose with the discovery that adipose tissue actively participates in homeostasis by secreting hormone-like substances such as the TNF and leptin. Both hormones can be seen as adipostat, in that their synthesis and secretion correlate with the increase of the size of the body fat depot (302 303 ).

Production of TNF by the immune system in response to a tumor or an infection leads to a considerable loss of adipose tissue and a waste of muscle that can result in cachexia. In obesity, the secretion of TNF by lipid-laden adipocytes also leads to increased lipolysis in adipocytes, generating an increase in circulating levels of FFA, whereas a diminished lipoprotein lipase activity decreases fatty acid uptake and thus decreases lipogenesis. Glycemia is also increased, due to the down-regulated expression of the glucose transporter Glut4 (304 ). TNF also counteracts insulin action by altering its signaling cascade (305 306 307 ). Direct support for the implication of TNF in the etiopathogeny of this insulin resistance syndrome comes from TNF null-mutant mice, which are protected from obesity-induced insulin resistance (308 ). Paradoxically, however, neutralization of the TNF receptors in mice results in hyperinsulinemia and decreased insulin sensitivity (309 ). A study in aging rats also suggests that the parallel increases of the adipose tissue-derived TNF activity and insulin resistance with age are not functionally linked (310 ), indicating that the pathway between TNF and insulin resistance is far from being understood.

The antagonism between TNF and PPAR appears at three levels. First, as previously mentioned, TNF is an inhibitor of adipocyte differentiation. These antiadipogenic effects of TNF most likely result from the down-regulation of PPAR1 and PPAR2 expression. This reduction precedes that of other adipocyte marker genes such as aP2 and C/EBP (311 ). Reciprocally, the insulin sensitizer TZD effectively opposes TNF-mediated repression of adipocyte genes (312 ). Second, PPAR activators partially reduce TNF-mediated lipolysis, but not that induced by catecholamines (313 ). Third, several mechanisms have been proposed that link the role of TNF in the insulin resistance syndrome to the relief of this pathology through TZD-mediated PPAR activation. They involve the normalization of TNF expression in white adipose tissue and in muscle (314 ), as well as a PPAR-mediated inhibition of the TNF-induced hypophosphorylation of the insulin receptor and insulin receptor substrate 1 (315 ).

Leptin, the product of the ob gene (316 ), is a 16-kDa protein that is secreted by adipocytes as an indicator of the size of energy stores in the adipose tissue (317 318 ). Indeed, high leptin levels in blood were shown to reflect body lipid content in humans and mice (319 320 ). A recent study demonstrates that leptin is also expressed by muscle cells in response to hyperglycemia or hyperlipidemia (321 ). One main target of leptin are the cells of the hypothalamic nuclei through which the hormone triggers both a down-regulation of food intake and an increase in energy expenditure (322 ). An alteration of this feedback mechanism as it occurs in mutations of the leptin receptor results in leptin resistance (318 ). It now appears that many other cells, including adipocytes, pancreatic cells, and muscle cells, possess the leptin membrane receptor that is encoded by the db gene. In adipocytes, leptin stimulates lipolysis and glucose utilization while in pancreatic ß-cells, leptin can decrease the expression and secretion of insulin. These effects can be counteracted by the inhibition of leptin production by TZD-activated PPAR (323 324 325 ). The proposed molecular mechanism for this inhibition implies a functional antagonism between C/EBP and PPAR on the leptin promoter activity (326 ) and is thought to participate in the TZD-mediated improvement of the insulin resistance syndrome.

Difficulties in understanding the respective role of PPAR, TNF, and leptin in the integrated pattern of responses to overfeeding and obesity come from observations in mice without fat tissue. In aP2/DTA mice, most of brown and white adipose tissue is progressively deleted via fat-specific expression of the DTA chain (327 ). Such mice have very low plasma levels of leptin, and thus are hyperphagic, but they do not gain weight. Mice A-ZIP/F-1 express a dominant-negative protein that impairs the function of the transcription factors of both the C/EBP and Jun families and are devoid of white fat throughout development. After an initial period of delayed growth, they gain weight but suffer from steatosis and enlarged organs (269 ). In both models, the inability to adequately store the ingested energy results in metabolic perturbations reminiscent of diabetes with hyperglycemia, hyperlipidemia, and hyperinsulinemia. Here, the insulin resistance syndrome is unlikely to be due to TNF and/or leptin production and signaling since adipose tissue is missing. However, in aP2/DTA mice, TZDs are still very efficient in normalizing glucose, lipids, and insulin blood values (328 ). These observations might be paralleled with results obtained in the Zucker diabetic fatty (ZDF) fa/fa rats. In this strain of rats that carry a mutation in the leptin receptor gene, a progressive obesity occurs with consequences resembling the insulin resistance found in non-insulin-dependent diabetes mellitus. In young animals, hyperinsulinemia compensates for insulin resistance, but when the animals become older, triglycerides overload the pancreatic islet ß-cells, which results in a decreased insulin production that may participate in a diabetes decompensation (329 ). In vitro, troglitazone treatment lowers fat content of pancreatic islets isolated from such rats and restores ß-cell function (330 ). Based on these observations, it would be of interest to analyze aP2/DTA mice at the late stage of the fat deletion process. These animals might also suffer from fat deposits in pancreatic ß-cells, whose function, as in fa/fa rats, could possibly be improved by TZD treatment. More interestingly, if troglitazone treatment indeed lowers the fat content of pancreatic islet cells, it might have the same effect in other cells. Overload of skeletal muscle cells with triglycerides and its metabolic consequences, such as reduced glucose utilization, could be corrected by the treatment. Such an adipose tissue-independent mechanism would explain the TZD-mediated improvement of insulin sensitivity in aP2/DTA mice. In support of this line of thought, relatively high PPAR expression in muscle cells has been observed in obese patients together with a TZD-mediated improvement of the insulin-dependent utilization of glucose by these cells (196 ). It has also been shown that treatment by TZD of human muscle cells in culture results in an increased expression of the PPAR protein (331 ), while an in vivo TZD treatment of mice and rats improves the insulin-stimulated glucose uptake in skeletal muscle (332 ). Alternatively, it is valid to question whether all TZD effects are mediated through PPAR only, or if other mechanisms are involved. It would not be unreasonable to envisage an action of TZD via a membrane receptor, since some PPAR ligands have a dual mode of action, through membrane and nuclear receptors. Furthermore, a direct or indirect role of the isotype PPAR in the ethiopathogeny of type 2 diabetes mellitus must also be considered. Unger’s group observations suggest that activation of PPAR in ZDF fa/fa rats is important for leptin signaling and maintenance of intracellular fatty acid homeostasis in pancreatic islets (179 ).

In summary, the connections that are appearing between PPAR, TNF, and leptin signaling might be only the tip of the iceberg of the hormonal control interregulating lipid and glucose homeostasis, from feeding behavior to basal metabolic activity. Progress in the understanding of these regulations will permit innovative and improved therapeutics for type 2 diabetes mellitus which affects massive proportions of the population in industrialized countries.

C. PPARs and control of inflammatory responses
Lipid mediators, particularly eicosanoids such as prostaglandins, leukotrienes, thromboxanes, and lipoxins, are involved in a variety of physiological processes including stimulation or inhibition of inflammation. Therapeutic control of an inflammatory response can be achieved either by blocking the membrane receptors mediating the action of inflammatory molecules or by modulating their metabolic fate through inhibition of their synthesis or stimulation of their breakdown. The first indication of a role of PPAR in controlling inflammation was the demonstration that LTB4, a potent chemotactic inflammatory eicosanoid whose activity is mediated by a membrane receptor (333 ), also binds to PPAR and induces transcription of genes of the - and ß-oxidation pathways that can neutralize and degrade LTB4 itself (65 ). In agreement with the above, dietary n-3 fatty acids and clofibrate, which also bind PPAR, have been reported to accelerate catabolism of LTB4 in granulocytes and macrophages (334 335 ). Conversely, PPAR-deficient mice show a prolonged inflammatory response when challenged with LTB4 or its precursor arachidonic acid, possibly due to the absence of stimulation of the catabolic pathways, hence, the increased duration of the inflammation (65 ). Inhibition of the synthesis of proinflammatory molecules such as interleukin 6 (IL-6) and prostaglandins by activated smooth muscle cells also appears to participate in PPAR-mediated control of inflammation (336 ), via a decreased activity of NF-B, a transcription factor regulating cytokine production. Another model is that of mouse aging where the levels of constitutively active NF-B increase in many tissues and are responsible for an elevated secretion of IL-6 and IL-12. With respect to these parameters, PPAR KO mice age prematurely, have increased NF-B expression in splenocytes, and present prematurely increased blood levels of constitutive and induced interleukins (337 ).

Recent studies demonstrate that PPAR too may have an important impact on inflammation, as treatment of activated macrophages with high doses of the PPAR ligand 15-deoxy-12,14-PGJ2 provokes a resting phenotype and inhibits the production of the inducible form of nitric oxide synthase and therefore nitric oxide, as well as that of gelatinase B and scavenger receptor A (338 ). This inhibition is due to an antagonizing activity of PPAR directed toward the activity of the transcription factors AP-1, STAT, and NF-B, which are known to control cytokine gene expression. Furthermore, treatment with PPAR ligands of human monocytes that have been exposed to phorbol ester inhibits the induced expression of TNF, IL-6, and IL-1ß (339 ). Thus, the antagonism between PPAR and TNF, discussed previously for the control of adipocyte differentiation, appears to occur also in inflammatory events. However, the involvement of additional, possibly PPAR-independent mechanisms, cannot be excluded (340 341 ).

The inhibition of cytokine production through PPAR activation might also contribute to the mechanism of action of the nonsteroidal antiinflammatory drugs (NSAIDs). These drugs are known to act by inhibiting cyclooxygenase activity (COX1 and COX2), thus blocking the production of proinflammatory prostaglandins. Indomethacin, a NSAID, also exhibits adipogenic activity at concentrations 100- to 1000-fold higher than that required for inhibition of COX activity. At these concentrations, often required in antiinflammatory treatments, NSAIDs are efficacious activators of PPAR and PPAR, consistent with their adipogenic and peroxisome proliferator activities (342 ). Therefore, a possible inhibition of cytokine production by PPAR might explain the incremental therapeutic benefit observed at high doses of these compounds. It is also possible that activation of PPAR at these relatively high drug concentrations contributes, in addition to COX inhibition, to the antiinflammatory, antipyretic, and analgesic properties of NSAIDs through stimulation of oxidative pathways neutralizing eicosanoids, similarly to the mechanism proposed for LTB4. In spite of these links between PPARs and some NSAIDs, it is noteworthy that other drugs of this type do not interact with PPARs, indicating that additional pathways operate for exerting their antiinflammatory properties.

As an interesting complement to these observations, fenofibrate treatment administered to hyperlipidemic patients not only lowers blood lipid values as previously discussed, but leads to a decrease in the blood of acute-phase proteins, whose levels of expression reflect systemic inflammation (336 ). This observation suggests that diets that modify PPAR activity and circulating lipid levels might also have a regulatory effect on inflammatory processes.

D. PPARs and atherosclerosis
Atherosclerosis is a pathological process that ultimately leads to the localized obstruction of an artery due to the progressive build-up in the arterial wall of an atheromatous plaque. At least three pathological processes participate in plaque formation: foam cell differentiation, inflammatory reaction, and cell proliferation (343 ). The passage of monocytes from the luminal endothelial surface to the subendothelial space where they differentiate into macrophages is the initial step. The presence of these resident macrophages in the intima of the vascular wall and high levels of LDL in the blood favor a modification of the LDL particles through oxidation or other poorly defined processes. Endocytosis of these particles by macrophages is then mediated by scavenger receptors. In contrast to the LDL receptor, these receptors are not down-regulated by the intracellular cholesterol content and thus allow an excessive accumulation of intracellular lipids resulting in the formation of lipid-laden foam cells. Cytokines produced by these activated macrophage/foam cells include the macrophage-colony stimulating factor, IL-1, and TNF, which form the basis of the inflammatory component of the atherosclerotic lesion and promote proliferation of smooth muscle cells. Necrosis of macrophages and lipid-loaded foam cells releases their intracellular contents, resulting in an accumulation of extracellular components that form the fibrous cap of the atheromatous lesion. Eventually, the rupture of this plaque leads to the acute arterial obstruction.

Many aspects of these pathological processes might be modulated by PPARs. We previously discussed the role of PPAR in the adipose differentiation program, which may present similarities with the formation of foam cells. We also presented PPAR-mediated regulation of circulating lipoprotein levels and cholesterol metabolism. In addition, attention has recently been given, using THP1 cells, to the activation of the monocyte-macrophage transition and the concomitant up-regulation of the CD36 scavenger receptor, whose gene is a direct PPAR target. Both phenomenons are under the positive control of PPAR which is itself up-regulated by oxidized LDL (344 345 ). Furthermore, expression of PPAR has indeed been demonstrated in mouse and human atherosclerotic lesions (345 346 ). In contrast to this apparently proatherosclerotic action of PPAR, inhibition of inflammatory cytokine production by the activated receptor might explain the beneficial effect of TZD in preventing atherosclerotic plaque progression. Similarly, inhibition of the macrophage activities by oxidized LDL (347 ), whose 9-HODE (9-hydroyxyoctadecadienoic acid) and 13-HODE components are PPAR ligands, has been observed (348 ). Obviously, further studies are needed to determine the exact role of PPAR in the development of atherosclerosis. Proliferation of aortic smooth muscle cells, which express both PPAR and PPAR, also likely contributes to both atherogenesis and restenosis processes. Activation of PPAR in these cells leads to a beneficial decrease of the phorbol 12-myristate 13-acetate-induced matrix metalloproteinase gene expression (349 ). In another study, activation of PPAR by its ligand inhibits COX2 expression and cytokine secretion through repression of AP-1, STAT, and NF-B signaling (336 ). It would now be of interest to clarify the respective role in vivo of the PPAR isotypes in such cells as well as evaluating possible regulatory roles of PPARs in vascular endothelial cells, where PPARs are also expressed (350 351 352 353 ).

In addition to cell necrosis, programmed cell death by apoptosis occurs in atherosclerotic lesions. Interestingly, treatment of differentiated macrophages with PPAR activators induces an apoptosis that is augmented when the cells are activated with interferon- and TNF (354 ). PPAR inhibits the transcriptional activity of the NFB p65/RelA subunit, suggesting that PPAR activators induce macrophage apoptosis by negatively interfering with the antiapoptotic NFB signaling pathway. However, it remains to be determined whether PPAR activator-induced apoptosis also occurs in vivo in the atherosclerotic lesion and subsequently what the consequences are for plaque formation.

E. PPARs and the development of the fetal epidermal permeability barrier
A particular aspect of lipid physiology, which is of interest with respect to PPAR biology, is found in the skin. The outermost layer of the epidermis, the stratum corneum, contains extracellular lipids delivered by exocytosis of lamellar bodies from epidermal granular cells. After subsequent processing into a matrix of lamellar unit structures, these extracellular lipids provide an efficient hydrophobic barrier to transepidermal water loss. Analysis by in situ hybridization of the mouse epidermis during development reveals distinct expression patterns of PPAR, -ß, and - as follows: in the mouse, PPARß is already expressed at E11.5, whereas no expression of PPAR or - is detected. Once the epidermis is multilayered, PPAR and - are expressed in all layers, whereas PPARß is present mainly in the basal layers. None of the three PPARs are detected in the adult epidermis by in situ hybridization (L. Michalik and W. Wahli, unpublished observations). In an in vitro model, differentiation of normal human keratinocytes exposed to calcium is accompanied by increased levels of PPAR and PPAR, whereas the level of PPARß remains unchanged (355 ). However, the differentiating medium is very important since PPARß strongly increases if a treatment with phorbol 12-myristate 13-acetate is used (356 ). Functional studies have shown that several hormones, including estrogen, glucocorticoid, thyroid hormone, and retinoids, affect epidermal maturation. Overexpression of a dominant negative RAR mutant in suprabasal cells during development results in a thick and loosely packed stratum corneum, which lacks the lipid multilamellar structure and is therefore an inefficient barrier, whereas overexpression in basal cells results in a thin epidermis and dry, scaly skin (357 358 ). It is thought that the overexpression of dominant negative RAR makes transcriptionally inactive heterodimers with RXR and might therefore subvert activities of other RXR partners such as PPARs, TRs, and VDR. Indeed, PPAR ligands such as oleic acid, linoleic acid and clofibrate accelerate epidermal development of fetal skin explants in vitro, resulting in mature lipid lamellar membranes forming a functional permeability barrier and a multilayered stratum corneum (359 ). PPAR activators also promote inhibition of proliferation and stimulate keratinocyte differentiation (360 ). At the biochemical level, activities of the enzymes steroid sulfatase and ß-glucocerebrosidase, linked to the barrier maturation, are increased after treatment with PPAR ligands. Since PPAR-selective ligands affect neither the development of barrier function nor epidermal morphology, PPAR or PPARß are more likely to be the isotypes involved, but a direct role remains to be demonstrated. Interestingly, additive effects on the epidermal development of fetal skin explants have been observed between activators of PPAR and the farnesol X-activated receptor (FXR), another binding partner of RXR (359 ). Both clofibrate and juvenile hormone III, a FXR activator, markedly accelerate fetal epidermal differentiation, sitmulating the expression of both profilaggrin/filaggrin and loricrin, which are structural proteins essential for stratum corneum formation. However, in explants treated with thyroid hormone, glucocorticoids, or estrogens, expression of these genes is also stimulated (361 ). Together these studies indicate a combined role of several nuclear receptors in epidermal maturation, which include, in addition to the classic ER, GR and TR, the receptors PPAR, RAR, and FXR and their heterodimerization partner RXR.

F. PPARs, carcinogenesis, and control of the cell cycle
A link between PPAR and cancer was first drawn after it became clear that peroxisome proliferators cause a dramatic increase in the incidence of liver tumors in mice and rats. Two major factors, an enhanced cell proliferation and an increased peroxisomal production of H₂O₂, have been implicated (362 363 ). Futhermore, nafenopin, a peroxisome proliferator, was shown to inhibit liver cell apoptosis in rat hepatocyte primary cultures, an effect that could also promote carcinogenesis (364 365 ). A comparative study of wild-type and PPAR KO mice fed with Wy-14,643 suggest that increased cyclin-dependent kinase 1, cyclin-dependent kinase-4, cyclin D1, and c-myc gene expressions might be directly or indirectly PPAR dependent (366 ). There are marked species differences in response to peroxisome proliferators, with mouse and rat being very prone to peroxisome proliferation, while other species, especially humans, are unresponsive (367 ). So far, no link has been found between PPAR activators and human hepatocarcinogenesis (368 ). These species differences could be due to interspecies variations in the expression of PPAR in liver, with levels of expression in humans being 1–10% of those found in mouse and rat (369 ). An alteration of the PPRE sequence in the human acyl-CoA oxidase gene might also explain the relative human unresponsiveness to PPAR ligands (370 ). Furthermore, there is evidence for structural polymorphism in hPPAR, but the biological significance of this observation, if any, is unclear. We also previously mentioned species-specific responses to some synthetic PPAR ligands, as analyzed in Xenopus, mouse, and human PPAR (67 371 ). However, more work is needed to assess how frequently such species differences occur. These differences underscore the care that must be taken when extrapolating results from standard toxicological testing of drugs in rodents to human physiology. Thus, although the PPAR-deficient mice, in which peroxisome proliferation cannot be induced any more, are invaluable for carcinogen bioassays aimed to assess to what extend PPAR is implicated, complementary approaches are wished for. For example, the generation of a mouse expressing hPPAR, would allow comparison of the role of the human protein itself to that of the mouse in a murine background and at murine expression levels.

A certain number of analyses suggest a role for PPAR in inducing cell growth arrest. In that respect, the physiological model of adipocyte conversion provides a valuable tool to study cell cycle arrest and terminal differentiation. For example, it has been shown that in addition to the coexpression of PPAR and C/EBP, withdrawal from the cell cycle is required for 3T3-L1 differentiation into adipocyte and involves the hypophosphorylation of the retinoblastoma susceptibility gene product Rb (372 ). However, activation of PPAR in Rb-/- mouse embryo fibroblasts is sufficient to induce adipocyte terminal differention (373 ) and, thus, the link between PPAR and Rb phosphorylation remains to be established. Cell cycle arrest of logarithmically growing fibroblasts and of SV40 large T antigen-transformed adipogenic HIB1B cells caused by ligand-activated PPAR have been associated with a loss of DNA binding and loss of activity of the growth-related transcription factor E2F/DP (374 ). 15-Deoxy-12,14-PGJ2 can also trigger the apoptosis of endothelial cells via a PPAR-dependent pathway (351 ). Studies based on malignant cells clearly support the concept of PPAR being implicated in cell cycle withdrawal. Primary human liposarcoma cells, which express high levels of PPAR (375 ), can be stimulated to undergo terminal differentiation by treatment with PPAR ligands or RXR-specific ligands. Simultaneous application of both treatments results in additive stimulation of differentiation, which is characterized by stimulation of adipocyte-specific genes, intracellular lipid accumulation, and withdrawal from the cell cycle (Ref. 375 ; see also Ref. 376 ). Activation of PPAR also induces reduction in growth rate and clonogenic capacity of human breast cancer cells in culture. In one of the breast cancer cell lines, which expresses high levels of PPAR, the resistance to TZD was associated with a high MAP kinase activity, which might explain a low PPAR activity due to phosphorylation as discussed previously (377 ). A similar analysis (378 ) has demonstrated that the inhibition of MCF7 clonal growth by troglitazone and by all-trans-RA is reversible when the compounds are used alone, but becomes irreversible when used in combination. This inhibition is accompanied by lipid accumulation, which, however, is not paralleled by an adipocyte differentiation gene expression pattern, but has been correlated with a profound decrease in bcl-2 gene expression and a marked increase in apoptosis. Interestingly, breast adenocarcinoma tissues from three human patients have responded similarly to the combined treatment when tested in culture (378 ). Human prostate cancer cells were found to express high levels of PPAR too, contrasting with the low expression in normal prostate tissue. In clonogenic assays with these cells, PPAR activators are efficient antiproliferators (379 ). Various human colon cancer cell lines express PPAR at high levels, and addition of a PPAR ligand not only reduces their clonogenic growth in culture but also decreases their growth when transplanted in nude mice (380 381 ). Interestingly, the antiangiogenic effect of PPAR ligands may also participate to growth inhibition (382 ).

In vivo studies, however, contrast with these results obtained from cells in culture or transplanted into nude mice. A protumor effect of PPAR has been recently described in mice bearing a mutation in the adenomatous polyposis Coli tumor suppressor gene. In such mice, treatment with PPAR agonists significantly increases the frequency and size of colon tumors (185 186 ). The discrepancy with the above mentioned results obtained with colon cancer cell lines does not seem to be attributable to the genetic defect that causes the tumors in mice, since some of these lines also bear this specific mutation (381 383 ).

In summary, one prominent feature found in the data so far reported is the high expression of PPAR in tumor cells. If this expression results from an attempt of the cancer cell to down-regulate its proliferative propensity, making PPAR activation beneficial for controlling the tumor, or from a dysregulated pathway linked to the tumor process, making PPAR activation an aggravation of the tumor environment, is still a matter of debate.

	IV. Conclusions

Top Abstract I. Introduction II. Molecular Aspects III. Physiological Aspects IV. Conclusions References

 
The discovery of PPARs and identification of fatty acids and their derivatives as ligands, a few years ago, have uncovered an unexpected and fascinating regulatory mode of action of lipids as direct modulators of gene expression. Since then, the excitement has not weakened while compelling evidence has accumulated that PPAR and PPAR act at crucial nodes of the regulatory network that achieve energy homeostasis in the organism. More specifically, an emerging picture is that of a dual and complementary role of PPAR and - isotypes in the regulation of the catabolic and anabolic aspects of lipid metabolism, respectively. Stimulating findings also include the discovery that lipid mediators, such as some eicosanoids (leukotrienes and prostaglandins), are natural PPAR ligands, opening new perspectives for investigating possible novel determinants of energy balance, as well as novel functions for PPARs, with links to glucose homeostasis, cell cycle control, inflammation, and immune response. As a corollary, PPARs are promising targets for therapeutic intervention, through the development of agonists but also antagonists, in disorders such as obesity and diabetes, atherosclerosis, chronic inflammatory diseases, and tumorigenesis.

However, one characteristic of the PPARs is that their activation can occur through a broad spectrum of ligands with rather low affinity. This implies that particular care must be taken when assessing the PPAR dependence of a given signaling pathway. More interestingly, some signals might be transduced by different ways, as exemplified by the subtle interplay between the membrane and nuclear receptors, introducing new levels of complexity in PPAR biology as determinants of the fine tuning of interconnected metabolic processes.

	Acknowledgments

 
We would like to thank Pascal Escher, Daniel Robyr, and Maria Klimek for their help in preparing the figures, and Christoph Meier, Sander Kersten, Nestor Fournier, Bernard Thorens, Sandrine Gremlich, and Liliane Michalik for reading the manuscript and/or for discussions. Furthermore, we are also grateful to Johan Auwerx, Pierre Chambon, Michael Milburn, Tim Willson, Noa Noy, Richard Rachubinski, and Kazuhiko Umesono for communicating results before publication.

	Footnotes

 
Address reprint requests to: Béatrice Desvergne M.D., Ph.D. or Walter Wahli, Ph.D., Batiment de Biologie, Institut de Biologie Animale, Universite de Lausanne, CH-1015 Lausanne, Switzerland.

¹ The work done in the authors’ laboratories was supported by grants from the Swiss National Science Foundation and the Etat de Vaud.

² Abbreviations used: ACO, acyl-CoA oxidase; ACS, acyl-CoA synthase; ADD1, adipocyte determination and differentiation factor 1; AF1, AF2, activation function 1 and 2; BMI, body mass index; CAP, c-Cbl-associated protein; CARLA, coactivator-dependent receptor ligand assay; CBP, CREB-binding protein; C/EBP, CAAT/enhancer binding protein; CPT, carnitine palmitoyl transferase; CTE, carboxy terminal extension; COX, cyclo-oxygenase; DBD, DNA binding domain; DPSA, differential protease sensitivity assay; DR1 and DR2, direct repeat with 1 bp or 2 bp spacing, respectively; DRIP, vitamin D receptor interacting protein; DTA, diphtheria toxin A; ER, estrogen receptor; ERE, estrogen response element; FA, fatty acid; FABP, fatty acid binding protein; FAS, fatty acid synthase; FAT, fatty acid translocase; FATP, fatty acid transporter protein; FXR, farnesol X-activated receptor; GR, glucocorticoid receptor; H1 to H12, helices 1 to 12 (in nuclear receptor LBD); HAF, helix comprising the activation function domain; HETE, hydroxyeicosatetraenoic acid; HNF4, hepatocyte nuclear factor 4; HDL, high-density lipoprotein; KO, knock-out; LBD, ligand binding domain; LDL, low density lipoprotein; LIC, ligand induced complex; LPL, lipoprotein lipase; LTB4, leukotriene B4; LXRs: liver X receptors; MAP, microtubule-associated protein; MCAD, medium-chain acyl CoA dehydrogenase; mHMG-CoAS, mitochondrial hydroxymethylglutaryl-CoA; N-CoR, nuclear receptor corepressor; NSAID, nonsteroidal antiinflammatory drug; PBP, PPAR-binding protein; PEPCK, phosphoenolpyruvate carboxykinase; PDGF, platelet-derived growth factor; PGC1: PPAR coactivator 1; PPAR, peroxisome proliferator-activated receptor; PPRE, peroxisome proliferator response element; PUFA, polyunsaturated fatty acid; RA, retinoic acid; RAR, retinoic acid receptor; RIP140, receptor interacting protein 140; RXR, retinoid X receptor; SMRT, silencing mediator for retinoid and thyroid hormone receptors; SRC-1, steroid receptor co-activator 1; SREBPs, sterol regulatory element-binding proteins; TLS-CHOP, translocation liposarcoma-C/EBP homologous protein; TNF, tumor necrosis factor; TR, thyroid hormone receptor; TRAP, thyroid hormone receptor associated protein; TZD, thiazolidinedione; UCP, uncoupling protein; VDR, vitamin D receptor; VLDL, very low-density lipoprotein; ZDF, Zucker diabetic fatty.

	References

Top Abstract I. Introduction II. Molecular Aspects III. Physiological Aspects IV. Conclusions References

 

1. Brown MS, Goldstein JL 1997 The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89:331–340[CrossRef][Medline]
2. Nuclear Receptors Nomenclature Committee 1999 A unified nomenclature system for the nuclear receptor superfamily. Cell 97:161–163[CrossRef][Medline]
3. Peet D, Janowski B, Mangelsdorf D 1998 The LXRs: a new class of oxysterol receptors. Curr Opin Genet Dev 8:571–575[CrossRef][Medline]
4. Xing G, Zhang L, Zhang L, Heynen T, Yoshikawa T, Smith M, Weiss S, Detera-Waldeigh S 1995 Rat PPAR contains a CGG triplet repeat and is prominently expressed in the thalamic nuclei. Biochem Biophys Res Commun 217:1015–1025[CrossRef][Medline]
5. Issemann I, Green S 1990 Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators. Nature 347:645–650[CrossRef][Medline]
6. Chen F, Law SW, O’Malley BW 1993 Identification of two mPPAR related receptors and evidence for the existence of five subfamily members. Biochem Biophys Res Commun 196:671–677[CrossRef][Medline]
7. Zhu Y, Alvares K, Huang Q, Rao MS, Reddy JK 1993 Cloning of a new member of the peroxisome proliferator-activated receptor gene family from mouse liver. J Biol Chem 268:26817–26820[Abstract/Free Full Text]
8. Kliewer SA, Forman BM, Blumberg B, Ong ES, Borgmeyer U, Mangelsdorf DJ, Umesono K, Evans RM 1994 Differential expression and activation of family murine peroxisome proliferator-activated receptors. Proc Natl Acad Sci USA 91:7355–7359[Abstract/Free Full Text]
9. Amri EZ, Bonino F, Ailhaud G, Abumrad NA, Grimaldi PA 1995 Cloning of a protein that mediates transcriptional effects of fatty acids in preadipocytes. Homology to peroxisome proliferator- activated receptors. J Biol Chem 270:2367–2371[Abstract/Free Full Text]
10. Göttlicher M, Widmark E, Li Q, Gustafsson J-A 1992 Fatty acids activate a chimera of the clofibric acid-activated receptor and the glucocorticoid receptor. Proc Natl Acad Sci USA 89:4653–4657[Abstract/Free Full Text]
11. Aperlo C, Pognonec P, Saladin R, Auwerx J, Boulukos KE 1995 cDNA cloning and characterization of the transcriptional activities of the hamster peroxisome proliferator-activated receptor haPPAR. Gene 162:297–302[CrossRef][Medline]
12. Schmidt A, Endo N, Rutledge SJ, Vogel R, Shinar D, Rodan GA 1992 Identification of a new member of the steroid hormone receptor superfamily that is activated by a peroxisome proliferator and fatty acids. Mol Endocrinol 6:1634–1641[Abstract/Free Full Text]
13. Sher T, Yi HF, McBride OW, Gonzalez FJ 1993 cDNA cloning, chromosomal mapping and functional characterization of the human peroxisome proliferator activated receptor. Biochemistry 32:5598–5604[CrossRef][Medline]
14. Greene ME, Blumberg B, McBride OW, Yi HF, Kronquist K, Kwan K, Hsieh L, Greene G, Nimer SD 1995 Isolation of the human peroxisome proliferator activated receptor gamma cDNA: expression in hematopoietic cells and chromosomal mapping. Gene Expr 4:281–299[Medline]
15. Dreyer C, Krey G, Keller H, Givel F, Helftenbein G, Wahli W 1992 Control of the peroxisomal ß-oxidation pathway by a novel family of nuclear hormone receptors. Cell 68:879–887[CrossRef][Medline]
16. Laudet V, Hanni C, Coll J, Catzeflis F, Stehelin D 1992 Evolution of the nuclear receptor gene superfamily. EMBO J 11:1003–1013[Medline]
17. Knoll AH 1992 The early evolution of eukaryotes - A geological perspective. Science 256:622–627[Abstract/Free Full Text]
18. Keese PK, Gibbs A 1992 Origins of genes - Big bang or continuous creation. Proc Natl Acad Sci USA 89:9489–9493[Abstract/Free Full Text]
19. Beamer BA, Negri C, Yen CJ, Gavrilova O, Rumberger JM, Durcan MJ, Yarnall DP, Hawkins AL, Griffin CA, Burns DK, Roth J, Reitman M, Shuldiner AR 1997 Chromosomal localization and partial genomic structure of the human peroxisome proliferator activated receptor- (hPPAR) gene. Biochem Biophys Res Commun 233:756–759[CrossRef][Medline]
20. Yoshikawa T, Brkanac Z, Dupont BR, Xing GQ, Leach RJ, Detera-Wadleigh SD 1996 Assignment of the human nuclear hormone receptor, NUC1 (PPAR), to chromosome 6p21.1-p21.2. Genomics 35:637–638[CrossRef][Medline]
21. Jones PS, Savory R, Barratt P, Bell AR, Gray TJ, Jenkins NA, Gilbert DJ, Copeland NG, Bell DR 1995 Chromosomal localisation, inducibility, tissue-specific expression and strain differences in three murine peroxisome proliferator-activated receptor genes. Eur J Biochem 233:219–226[Medline]
22. Gearing KL, Crickmore A, Gustafsson J-Å 1994 Structure of the mouse peroxisome proliferator activated receptor gene. Biochem Biophys Res Commun 199:255–263[CrossRef][Medline]
23. Krey G, Keller H, Mahfoudi A, Medin J, Ozato K, Dreyer C, Wahli W 1993 Xenopus peroxisome proliferator activated receptors: genomic organization, response element recognition, heterodimer formation with retinoid x receptor and activation by fatty acids. J Steroid Biochem Mol Biol 47:65–73[CrossRef][Medline]
24. Zhu Y, Qi C, Korenberg JR, Chen XN, Noya D, Rao MS, Reddy JK 1995 Structural organization of mouse peroxisome proliferator-activated receptor (mPPAR) gene: alternative promoter use and different splicing yield two mPPAR isoforms. Proc Natl Acad Sci USA 92:7921–7925[Abstract/Free Full Text]
25. Fajas L, Auboeuf D, Raspe E, Schoonjans K, Lefebvre AM, Saladin R, Najib J, Laville M, Fruchart JC, Deeb S, Vidal-Puig A, Flier J, Briggs MR, Staels B, Vidal H, Auwerx J 1997 The organization, promoter analysis, and expression of the human PPAR- gene. J Biol Chem 272:18779–18789[Abstract/Free Full Text]
26. Fajas L, Fruchart JC, Auwerx J 1998 PPARgamma3 mRNA: a distinct PPAR mRNA subtype transcribed from an independent promoter. FEBS Lett 438:55–60[CrossRef][Medline]
27. Glass CK 1994 Differential recognition of target genes by nuclear receptor monomers, dimers, and heterodimers. Endocr Rev 15:391–407[Abstract/Free Full Text]
28. Kliewer SA, Umesono K, Noonan DJ, Heyman RA, Evans RM 1992 Convergence of 9-cis retinoic acid and peroxisome proliferator signalling pathways through heterodimer formation of their receptors. Nature 358:771–774[CrossRef][Medline]
29. Tugwood JD, Issemann I, Anderson RG, Bundell KR, McPheat WL, Green S 1992 The mouse peroxisome proliferator activated receptor recognizes a response element in the 5' flanking sequence of the rat acyl CoA oxidase gene. EMBO J 11:433–439[Medline]
30. Palmer CNA, Hsu M-H, Griffin KJ, Johnson EF 1995 Novel sequence determinants in peroxisome proliferator signaling. J Biol Chem 270:16114–16121[Abstract/Free Full Text]
31. IJpenberg A, Jeannin E, Wahli W, Desvergne B 1997 Polarity and specific sequence requirements of PPAR-RXR heterodimer binding to DNA: a functional analysis of the malic enzyme gene PPRE. J Biol Chem 272:20108–20117[Abstract/Free Full Text]
32. Juge-Aubry C, Pernin A, Favez T, Burger AG, Wahli W, Meier CA, Desvergne B 1997 DNA binding properties of peroxisome proliferator-activated receptor subtypes on various natural peroxisome proliferator response elements: importance of the 5' flanking region. J Biol Chem 272:25252–25259[Abstract/Free Full Text]
33. Osada S, Tsukamoto T, Takiguchi M, Mori M, Osumi T 1997 Identification of an extended half-site motif required for the function of peroxisome proliferator-activated receptor alpha. Genes to Cells 2:315–327[Abstract]
34. DiRenzo J, Soderstrom M, Kurokawa R, Ogliastro MH, Ricote M, Ingrey S, Horlein A, Rosenfeld MG, Glass CK 1997 Peroxisome proliferator-activated receptors and retinoic acid receptors differentially control the interactions of retinoid x receptor heterodimers with ligands, coactivators, and corepressors. Mol Cell Biol 17:2166–2176[Abstract]
35. Mangelsdorf DJ, Evans RM 1995 The RXR heterodimers and orphan receptors. Cell 83:841–850[CrossRef][Medline]
36. Hsu MH, Palmer CNA, Song W, Griffin KJ, Johnson EF 1998 A carboxyl-terminal extension of the zinc finger domain contributes to the specificity and polarity of peroxisome proliferator-activated receptor DNA binding. J Biol Chem 273:27988–27997[Abstract/Free Full Text]
37. Perlmann T, Rangara PN, Umesono K, Evans RM 1993 Determinants for selective RAR and TR recognition of direct repeat HREs. Genes Dev 7:1411–1422[Abstract/Free Full Text]
38. Kurokawa R, Yu VC, Näär A, Kyakumoto S, Han Z, Silverman S, Rosenfeld MG, Glass CK 1993 Differential orientations of the DNA-binding domain and carboxy-terminal dimerization interface regulate binding site selection by nuclear receptor heterodimers. Genes Dev 7:1423–1435[Abstract/Free Full Text]
39. Mader S, Chen JY, Chen ZP, White J, Chambon P, Gronemeyer H 1993 The patterns of binding of RAR, RXR and TR homodimers and heterodimers to direct repeats are dictated by the binding specificities of the DNA binding domains. EMBO J 12:5029–5041[Medline]
40. Lee MS, Kliewer SA, Provencal J, Wright PE, Evans RM 1993 Structure of the retinoid x receptor DNA binding domain: a helix required for homodimeric DNA binding. Science 260:1117–1121[Abstract/Free Full Text]
41. Towers TL, Luisi BF, Asianov A, Freedman LP 1993 DNA target selectivity by the vitamin-D3 receptor: mechanism of dimer binding to an asymmetric repeat element. Proc Natl Acad Sci USA 90:6310–6314[Abstract/Free Full Text]
42. Predki PF, Zamble D, Sarkar B, Giguère V 1994 Ordered binding of retinoic acid and retinoid x receptors to asymmetric response elements involves determinants adjacent to the DNA-binding domain. Mol Endocrinol 8:31–39[Abstract/Free Full Text]
43. Zechel C, Shen X-Q, Chen J-Y, Chen Z-P, Chambon P, Gronemeyer H 1994 The dimerization interfaces formed between the DNA binding domains of RXR, RAR and TR determine the binding specificity and polarity of the full-length receptors to direct repeats. EMBO J 13:1425–1433[Medline]
44. Zechel C, Shen X-Q, Chambon P, Gronemeyer H 1994 Dimerization interfaces formed between the DNA binding domains determine the cooperative binding of RXR/RAR and RXR/TR heterodimers to DR5 and DR4 elements. EMBO J 13:1414–1424[Medline]
45. Rastinejad F, Perlmann T, Evans RM, Sigler PB 1995 Structural determinants of nuclear receptor assembly on DNA direct repeats. Nature 375:203–211[CrossRef][Medline]
46. Zhao Q, Khorasanizadeh S, Miyoshi Y, Lazar MA, Rastinejad F 1998 Structural elements of an orphan nuclear receptor-DNA complex. Mol Cell 1:849–861[CrossRef][Medline]
47. Gervois P, Chopin-Delannoy S, Fadel A, Dubois G, Kosykh V, Fruchart JC, Naj‘b J, Laudet V, Staels B 1999 Fibrates increase human REV-ERBalpha expression in liver via a novel peroxisome proliferator-activated receptor response element. Mol Endocrinol 13:400–409[Abstract/Free Full Text]
48. Nakshatri H, Chambon P 1994 The directly repeated RG(G/T)TCA motifs of the rat and mouse cellular retinol-binding protein II genes are promiscuous binding sites for RAR, RXR, HNF-4 and ARP-1 homo- and heterodimers. J Biol Chem 269:890–902[Abstract/Free Full Text]
49. Fraser JD, Martinez V, Straney R, Briggs MR 1998 DNA binding and transcription activation specificity of hepatocyte nuclear factor 4. Nucleic Acids Res 26:2702–2707[Abstract/Free Full Text]
50. Baes M, Castelein H, Desmet L, Declercq PE 1995 Antagonism of COUP-TF and PPAR/RXR on the activation of the malic enzyme gene promoter - modulation by 9-cis RA. Biochem Biophys Res Commun 215:338–345[CrossRef][Medline]
51. Miyata KS, Zhang B, Marcus SL, Capone JP, Rachubinski RA 1993 Chicken ovalbumin upstream promoter transcription factor (COUP - TF) binds to a peroxisome proliferator-responsive element and antagonizes peroxisome proliferator-mediated signalling. J Biol Chem 268:19169–19172[Abstract/Free Full Text]
52. Winrow CJ, Marcus SL, Miyata KS, Zhang B, Capone JP, Rachubinski RA 1994 Transactivation of the peroxisome proliferator-activated receptor is differentially modulated by hepatocyte nuclear factor-4. Gene Expr 4:53–62[Medline]
53. Hertz R, Seckbach M, Zakin MM, Bar-Tana J 1996 Transcriptional suppression of the transferrin gene by hypolipidemic peroxisome proliferators. J Biol Chem 271:218–224[Abstract/Free Full Text]
54. Nakhastri H, Bhat-Nakshatri P 1998 Multiple parameters determine the specificity of transcriptional response by nuclear receptors HNF-4, ARP-1, PPAR, RAR, RXR through common response elements. Nucleic Acids Res 26:2491–2499[Abstract/Free Full Text]
55. Keller H, Givel F, Perroud M, Wahli W 1995 Signaling cross-talk between peroxisome proliferator-activated receptor/retinoid X receptor and estrogen receptor through estrogen response elements. Mol Endocrinol 9:794–804[Abstract/Free Full Text]
56. Nunez SB, Medin JA, Braissant O, Kemp L, Wahli W, Ozato K, Segars JH 1997 Retinoid x receptor and peroxisome proliferator-activated receptor activate an estrogen responsive gene independent of the estrogen receptor. Mol Cell Endocrinol 127:27–40[CrossRef][Medline]
57. Chu R, Madison LD, Lin Y, Kopp P, Rao MS, Jameson JL, Reddy JK 1995 Thyroid hormone (T3) inhibits ciprofibrate-induced transcription of gene encoding ß-oxidation enzymes: cross-talk between peroxisome proliferator and T3 signaling pathways. Proc Natl Acad Sci USA 92:11593–11597[Abstract/Free Full Text]
58. Juge-Aubry CE, Gorla-Bajszczak A, Pernin A, Lemberger T, Wahli W, Burger AG, Meier CA 1995 Peroxisome proliferator-activated receptor mediates cross-talk with thyroid hormone receptor by competition for retinoid X receptor: possible role of a leucine zipper-like heptad repeat. J Biol Chem 270:18117–18122[Abstract/Free Full Text]
59. Jow L, Mukherjee R 1995 The human peroxisome proliferator-activated receptor (PPAR) subtype NUC1 represses the activation of hPPAR and thyroid hormone receptors. J Biol Chem 270:3836–3840[Abstract/Free Full Text]
60. Tolon R, Castillo A, Aranda A 1998 Activation of the prolactin gene by peroxisome-proliferator activated receptor- appears to be DNA binding-independent. J Biol Chem 273:26652–26661[Abstract/Free Full Text]
61. Keller H, Dreyer C, Medin J, Mahfoudi A, Ozato K, Wahli W 1993 Fatty acids and retinoids control lipid metabolism through activation of peroxisome proliferator-activated receptor-retinoid X receptor heterodimers. Proc Natl Acad Sci USA 90:2160–2164[Abstract/Free Full Text]
62. Lehmann JM, Moore LB, Smith-Oliver TA, Wilkison WO, Willson TM, Kliewer SA 1995 An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor (PPAR). J Biol Chem 270:12953–12956[Abstract/Free Full Text]
63. Kliewer SA, Lenhard JM, Willson TM, Patel I, Morris DC, Lehmann JM 1995 A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor and promotes adipocyte differentiation. Cell 83:813–819[CrossRef][Medline]
64. Forman BM, Tontonoz P, Chen J, Brun RP, Spiegelman BM, Evans RM 1995 15-Deoxy-12,14-prostaglandin J2 is a ligand for the adipocyte determination factor PPAR. Cell 83:803–812[CrossRef][Medline]
65. Devchand PR, Keller H, Peters JM, Vazquez M, Gonzalez FJ, Wahli W 1996 The PPAR-leukotriene B4 pathway to inflammation control. Nature 384:39–43[CrossRef][Medline]
66. Kliewer SA, Sundseth SS, Jones SA, Brown PJ, Wisely GB, Koble CS, Devchand P, Wahli W, Willson TM, Lenhard JM, Lehmann JM 1997 Fatty acids and eicosanoids regulate gene expression through direct interactions with peroxisome proliferator-activated receptors and . Proc Natl Acad Sci USA 94:4318–4323[Abstract/Free Full Text]
67. Keller HJ, Devchand PR, Perroud M, Wahli W 1997 PPAR structure-function relationships derived from species-specific differences in responsiveness to hypolipidemic agents. Biol Chem 378:651–655[Medline]
68. Kersten S, Wahli W 1999 Adopting orphans: finding ligands. In: Picard D (ed) Nuclear Receptor: A Practical Approach. Oxford University Press, Oxford, UK, pp 71–93
69. Bosworth N, Towers P 1989 Scintillation proximity assay. Nature 341:167–168[CrossRef][Medline]
70. Nichols JS, Parks DJ, Consler TG, Blanchard SG 1998 Development of a scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding domain. Anal Biochem 257:112–119[CrossRef][Medline]
71. Dowell P, Peterson VJ, Zabriskie TM, Leid M 1997 Ligand-induced peroxisome proliferator-activated receptor conformational change. J Biol Chem 272:2013–2020[Abstract/Free Full Text]
72. Forman BM, Chen J, Evans RM 1997 Hypolipidemic drugs, polyunsaturated fatty acids and eicosanoids are ligands for PPAR and PPAR. Proc Natl Acad Sci USA 94:4312–4317[Abstract/Free Full Text]
73. Krey G, Braissant O, L’Horset F, Kalkhoven E, Perroud M, Parker MG, Wahli W 1997 Fatty acids, eicosanoids and hypolipidemic agents identified as ligands of peroxisome proliferator-activated receptors by CARLA. Mol Endocrinol 11:779–791[Abstract/Free Full Text]
74. Berger J, Leibowitz MD, Doebber TW, Elbrecht A, Zhang B, Zhou G, Biswas C, Cullinan CA, Hayes NS, Li Y, Tanen M, Ventre J, Wu MS, Berger GD, Mosley R, Marquis R, Santini C, Sahoo SP, Tolman RL, Smith RG, Moller DE 1999 Novel peroxisome proliferator-activated receptor (PPAR) and PPAR ligands produce distinct biological effects. J Biol Chem 274:6718–6725[Abstract/Free Full Text]
75. Willson TM, Wahli W 1997 Peroxisome proliferator-activated receptor (PPAR) agonists. Curr Opin Chem Biol 1:235–241[CrossRef][Medline]
76. Johnson TE, Holloway MK, Vogel R, Rutledge SJ, Perkins JJ, Rodan GA, Schmidt A 1997 Structural requirements and cell-type specificity for ligand activation of peroxisome proliferator-activated receptors. J Steroid Biochem Mol Biol 63:1–8[CrossRef][Medline]
77. Ellinghaus P, Wolfrum C, Assmann G, Spener F, Seedorf U 1999 Phytanic acid activates the peroxisome proliferator-activated receptor (PPAR) in sterol carrier protein2-/sterol carrier protein x-deficient mice. J Biol Chem 274:2766–2772[Abstract/Free Full Text]
78. Devchand PR, Wahli W 1998 Leukotriene B4: agonist for the fat regulator PPAR. In: Rodger I, Botting J, Dahlén S-E (eds) Leukotrienes: New Concepts and Targets for Therapy. Kluwer Academic Publishers and William Harvey Press, pp 119–124
79. Devchand PR, Hihi AK, Perroud M, Schleuning WD, Spiegelman BM, Wahli W 1999 Chemical probes that differentially modulate PPAR and BLTR, nuclear and cell surface receptors for leukotriene B4. J Biol Chem 274:23341–23348
80. Brock TG, McNish RW, Peters GM 1995 Translocation and leukotriene synthetic capacity of nuclear 5-lipoxygenase in rat basophilic leukemia cells and alveolar macrophages. J Biol Chem 270:21652–21658[Abstract/Free Full Text]
81. Chen X-S, Zhang Y-Y, Funk C 1998 Determinants of 5-lipoxygenase nuclear localisation using green fluorescent protein/5-lipoxygenase fusion proteins. J Biol Chem 273:31237–31244[Abstract/Free Full Text]
82. Palmer CN, Wolf CR 1998 cis-Parinaric acid is a ligand for the human peroxisome proliferator activated receptor : development of a novel spectrophotometric assay for the discovery of PPAR ligands. FEBS Lett 431:476–480[CrossRef][Medline]
83. Lin Q, Ruuska SE, Shaw NS, Dong D, Noy N 1999 Ligand selectivity of the peroxisome proliferator-activated receptor . Biochemistry 38:185–190[CrossRef][Medline]
84. Berger J, Bailey P, Biswas C, Cullinan CA, Doebber TW, Hayes NS, Saperstein R, Smith RG, Leibowitz MD 1996 Thiazolidinediones produce a conformational change in peroxisomal proliferator-activated receptor-: binding and activation correlate with antidiabetic actions in db/db mice. Endocrinology 137:4189–4195[Abstract]
85. Henke BR, Blanchard SG, Brackeen MF, Brown KK, Cobb JE, Collins JL, Harrington WWJ, Hashim MA, Hull-Ryde EA, Kaldor I, Kliewer SA, Lake DH, Leesnitzer LM, Lehmann JM, Lenhard JM, Orband-Miller LA, Miller JF, Mook RAJ, Noble SA, Oliver WJ, Parks DJ, Plunket KD, Szewczyk JR, Willson TM 1998 N-(2-benzoylphenyl)-L-tyrosine PPAR agonists. 1. Discovery of a novel series of potent antihyperglycemic and antihyperlipidemic agents. J Med Chem 41:5020–5036[CrossRef][Medline]
86. Brown PJ, Smith-Oliver TA, Charifson PS, Tomkinson NCO, Fivush AM, Sternbach DD, Wade LE, Orband-Miller L, Parks D, Blanchard SG, Kliewer SA, Lehmann JM, Willson TM 1997 Identification of peroxisome proliferator-activated receptor ligands from a biased chemical library. Chem Biol 4:909–918[CrossRef][Medline]
87. Bourguet W, Ruff M, Chambon P, Gronemeyer H, Moras D 1995 Crystal structure of the ligand-binding domain of the human nuclear receptor RXR. Nature 375:377–382[CrossRef][Medline]
88. Renaud JP, Rochel N, Ruff M, Vivat V, Chambon P, Gronemeyer H, Moras D 1995 Crystal structure of the RAR-gamma ligand-binding domain bound to all-trans retinoic acid. Nature 378:681–689[CrossRef][Medline]
89. Wagner RL, Apriletti JW, McGrath ME, West BL, Baxter JD, Fletterick RJ 1995 A structural role for hormone in the thyroid hormone receptor. Nature 378:690–697[CrossRef][Medline]
90. Xu HE, Lambert MH, Montana VG, Parks DJ, Blanchard SG, Brown PJ, Sternbach DD, Lehmann JM, Wisely GB, Willson TM, Kliewer SA, Milburn MV 1999 Molecular recognition of fatty acids by peroxisome proliferator-activated receptors. Mol Cell 3:397–403[CrossRef][Medline]
91. Nolte RT, Wisely GB, Westin S, Cobb JE, Lambert MH, Kurokawa R, Rosenfeld MG, Willson TM, Glass CK, Millburn MV 1998 Ligand binding and co-activator assembly of the peroxisome proliferator-activated receptor-. Nature 395:137–143[CrossRef][Medline]
92. Uppenberg J, Svensson C, Jaki M, Bertilsson G, Jendeberg L, Berkenstam A 1998 Crystal structure of the ligand binding domain of the human nuclear receptor PPAR. J Biol Chem 273:31108–31112[Abstract/Free Full Text]
93. Hsu MH, Palmer CN, Griffin KJ, Johnson EF 1995 A single amino acid change in the mouse peroxisome proliferator-activated receptor alpha alters transcriptional responses to peroxisome proliferators. Mol Pharmacol 48:559–567[Abstract]
94. Shalev A, Siegrist-Kaiser CA, Yen PM, Wahli W, Burger AG, Chin WW, Meier CA 1996 The peroxisome proliferator-activated receptor is a phosphoprotein: regulation by insulin. Endocrinology 137:4499–4502[Abstract]
95. Juge-Aubry CE, Hammar E, Siegrist-Kaiser C, Pernin A, Takeshita A, Chin WW, Burger AG, Meier CA 1999 Regulation of the transcriptional activity of the peroxisome proliferator-activated receptor by phosphorylation of a ligand-independent trans- activating domain. J Biol Chem 274:10505–10510[Abstract/Free Full Text]
96. Zhang B, Berger J, Zhou G, Elbrecht A, Biswas S, White-Carrington S, Szalkowski D, Moller DE 1996 Insulin- and mitogen-activated protein kinase-mediated phosphorylation and activation of peroxisome proliferator-activated receptor . J Biol Chem 271:31771–31774[Abstract/Free Full Text]
97. Camp HS, Tafuri SR 1997 Regulation of peroxisome proliferator-activated receptor activity by mitogen-activated protein kinase. J Biol Chem 272:10811–10816[Abstract/Free Full Text]
98. Hu E, Kim JB, Sarraf P, Spiegelman BM 1996 Inhibition of adipogenesis through MAP kinase-mediated phosphorylation of PPAR. Science 274:2100–2103[Abstract/Free Full Text]
99. Camp H, Tafuri S, Leff T 1999 c-Jun N-terminal kinase phosphorylates peroxisome proliferator-activated receptor-1 and negatively regulates its transcriptional activity. Endocrinology 140:392–397[Abstract/Free Full Text]
100. Reginato MJ, Krakow SL, Bailey ST, Lazar MA 1998 Prostaglandins promote and block adipogenesis through opposing effects on peroxisome proliferator-activated receptor . J Biol Chem 273:1855–1858[Abstract/Free Full Text]
101. Shao D, Rangwala SM, Bailey ST, Krakow SL, Reginato MJ, Lazar MA 1998 Interdomain communication regulating ligand binding by PPAR-. Nature 396:377–380[CrossRef][Medline]
102. Gabbay RA, Sutherland C, Gnudi L, Kahn BB, O’Brien RM, Granner DK, Flier JS 1996 Insulin regulation of phosphoenolpyruvate carboxykinase gene expression does not require activation of the Ras/mitogen-activated protein kinase signaling pathway. J Biol Chem 271:1890–1897[Abstract/Free Full Text]
103. Zhou Y-C, Waxman D 1999 Cross-talk between Janus kinase-signal transducer and activator of transcription (JAK-STAT) and peroxisome proliferator-activated receptor- (PPAR) signaling pathways. J Biol Chem 274:2672–2681[Abstract/Free Full Text]
104. Gearing KL, Göttlicher M, Teboul M, Widmark E, Gustafsson JA 1993 Interaction of the peroxisome-proliferator-activated receptor and retinoid X-receptor. Proc Natl Acad Sci USA 90:1440–1444[Abstract/Free Full Text]
105. Vu-Dac N, Schoonjans K, Kosykh V, Dallongeville J, Heyman RA, Staels B, Auwerx J 1996 Retinoids increase human apolipoprotein A-II expression through activation of the retinoid X receptor but not the retinoic acid receptor. Mol Cell Biol 16:3350–3360[Abstract]
106. Poirier H, Braissant O, Niot I, Wahli W, Besnard P 1997 9-cis-retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene. FEBS Lett 412:480–484[CrossRef][Medline]
107. Mukherjee R, Davies PJA, Cromble DL, Bischoff ED, Cesario RM, Jow L, Hamann LG, Boehm MF, Mondon CE, Nadzan AM, Paterniti Jr JR, Heyman RA 1997 Sensitization of diabetic and obese mice to insulin by retinoid X receptor agonists. Nature 386:407–410[CrossRef][Medline]
108. Mukherjee R, Strasser J, Jow L, Hoener P, Paterniti Jr JR, Heyman RA 1998 RXR agonists activate PPAR-inducible genes, lower triglycerides, and raise HDL levels in vivo. Arterioscler Thromb Vasc Biol 18:272–276[Abstract/Free Full Text]
109. Mertz JR, Shang E, Piantedosi R, Wei S, Wolgemuth DJ, Blaner WS 1997 Identification and characterization of a stereospecific human enzyme that catalyzes 9-cis-retinol oxidation. A possible role in 9-cis-retinoic acid formation. J Biol Chem 272:11744–11749[Abstract/Free Full Text]
110. Romert A, Tuvendal P, Simon A, Dencker L, Eriksson U 1998 The identification of a 9-cis retinol dehydrogenase in the mouse embryo reveals a pathway for synthesis of 9-cis retinoic acid. Proc Natl Acad Sci USA 95:4404–4409[Abstract/Free Full Text]
111. Forman BM, Umesono K, Chen J, Evans RM 1995 Unique response pathways are established by allosteric interactions among nuclear hormone receptors. Cell 81:541–550[CrossRef][Medline]
112. Perlmann T, Jansson L 1995 A novel pathway for vitamin A signaling mediated by RXR heterodimerization with NGFI-B and NURR1. Genes Dev 9:769–782[Abstract/Free Full Text]
113. Willy PJ, Umesono K, Ong ES, Evans RM, Heyman RA, Mangelsdorf DJ 1995 LXR, a nuclear receptor that defines a distinct retinoid response pathway. Genes Dev 9:1033–1045[Abstract/Free Full Text]
114. Kersten S, Kelleher D, Chambon P, Gronemeyer H, Noy N 1995 Retinoid x receptor forms tetramers in solution. Proc Natl Acad Sci USA 92:8645–8649[Abstract/Free Full Text]
115. Dong D, Noy N 1998 Heterodimer formation by retinoid x receptor: regulation by ligands and by the receptor’s self-association properties. Biochemistry 37:10691–10700[CrossRef][Medline]
116. Mangelsdorf DJ, Umesono K, Kliewer SA, Borgmeyer U, Ong ES, Evans RM 1991 A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR. Cell 66:555–561[CrossRef][Medline]
117. Peters JM, Hennuyer N, Staels B, Fruchart JC, Fievet C, Gonzalez FJ, Auwerx J 1997 Alterations in lipoprotein metabolism in peroxisome proliferator-activated receptor -deficient mice. J Biol Chem 272:27307–27312[Abstract/Free Full Text]
118. Vu-Dac N, Gervois P, Torra IP, Fruchart J-C, Kosykh V, Kooistra T, Princen HMG, Dallongeville J, Staels B 1998 Retinoids increase human Apo-CIII expression at the transcriptional level via the retinoid X receptor. J Clin Invest 102:625–632[Medline]
119. Canan Koch SS, Dardashti LJ, Hebert JJ, White SK, Croston GE, Flatten KS, Heyman RA, Nadzan AM 1996 Identification of the first retinoid X receptor homodimer antagonist. J Med Chem 39:3229–3234[CrossRef][Medline]
120. Lala DS, Mukherjee R, Schulman IG, Koch SS, Dardashti LJ, Nadzan AM, Croston GE, Evans RM, Heyman RA 1996 Activation of specific RXR heterodimers by an antagonist of RXR homodimers. Nature 383:450–453[CrossRef][Medline]
121. Chambon P 1996 A decade of molecular biology of retinoic acid receptors. FASEB J 10:940–954[Abstract]
122. Minucci S, Leid M, Toyama R, Saint-Jeannet JP, Peterson VJ, Horn V, Ishmael JE, Bhattacharyya N, Dey A, Dawid IB, Ozato K 1997 Retinoid x receptor (RXR) within the RXR-retinoic acid receptor heterodimer binds its ligand and enhances retinoid-dependent gene expression. Mol Cell Biol 17:644–655[Abstract]
123. Vivat V, Zechel C, Wurtz J-M, Bourguet W, Kagechika H, Umemiya H, Shudo K, Moras D, Gronemeyer H, Chambon P 1997 A mutation mimicking ligand-induced conformational change yields a constitutive RXR that senses allosteric effects in heterodimers. EMBO J 16:5697–5709[CrossRef][Medline]
124. Werman A, Hollenberg A, Solanes G, Bjorbaek C, Vidal-Puig AJ, Flier JS 1997 Ligand-independent activation domain in the N terminus of peroxisome proliferator-activated receptor (PPAR). J Biol Chem 272:20230–20235[Abstract/Free Full Text]
125. Adams M, Reginato MJ, Shao D, Lazar MA, Chatterjee VK 1997 Transcriptional activation by peroxisome proliferator-activated receptor is inhibited by phosphorylation at a consensus mitogen-activated protein kinase site. J Biol Chem 272:5128–5132[Abstract/Free Full Text]
126. Ristow M, Muller-Wieland D, Pfeiffer A, Krone W, Kahn CR 1998 Obesity associated with a mutation in a genetic regulator of adipocyte differentiation. N Engl J Med 339:953–959[Abstract/Free Full Text]
127. Glass CK, Rose DW, Rosenfeld MG 1997 Nuclear receptor coactivators. Curr Opin Cell Biol 9:222–232[CrossRef][Medline]
128. Horwitz KB, Jackson TA, Bain DL, Richer JK, Takimoto GS, Tung L 1996 Nuclear receptor coactivators and corepressors. Mol Endocrinol 10:1167–1177[Abstract/Free Full Text]
129. Zamir I, Zhang J, Lazar M 1997 Stoichiometric and steric principles governing repression by nuclear hormone receptors. Genes Dev 11:835–846[Abstract/Free Full Text]
130. Oñate SA, Tsai SY, Tsai MJ, O’Malley BW 1995 Sequence and characterization of a coactivator for the steroid hormone receptor superfamily. Science 270:1354–1357[Abstract/Free Full Text]
131. Zhu Y, Qi C, Calandra C, Rao M, Reddy J 1996 Cloning and identification of mouse steroid receptor coactivator-1 (mSRC-1), as a coactivator of peroxisome proliferator-activated receptor . Gene Expr 6:185–195[Medline]
132. Heery DM, Kalkhoven E, Hoare S, Parker MG 1997 A signature motif in transcriptional co-activators mediates binding to nuclear receptor. Nature 387:733–736[CrossRef][Medline]
133. McInerney EM, Rose DW, Flynn SE, Westin S, Mullenm TM, Krones A, Inostroza J, Torchia J, Nolte RT, Assa-Munt N, Milburn MV, Glass CK, Rosenfeld MG 1998 Determinants of coactivator LXXLL motif specificity in nuclear receptor transcriptional activation. Genes Dev 12:3357–3368[Abstract/Free Full Text]
134. Zhou G, Cummings R, Li Y, Mitra S, Wilkinson HA, Elbrecht A, Hermes D, Schaeffer JM, Smith RG, Moller DE 1998 Nuclear receptors have distinct affinities for coactivators: characterization by fluorescence resonance energy transfer. Mol Endocrinol 12:1594–1604[Abstract/Free Full Text]
135. Westin S, Kurokawa R, Nolte RT, Wisely GB, Mclnerney EM, Rose DW, Milburn MV, Rosenfeld MG, Glass CK 1998 Interactions controlling the assembly of nuclear-receptor heterodimers and co-activators. Nature 395:199–202[CrossRef][Medline]
136. Qi C, Zhu Y, Yeldandi AV, Rao MS, Maeda N, Subbarao V, Pulikuri S, Hashimoto T, Reddy JK 1999 Mouse steroid receptor coactivator-1 is not essential for peroxisome proliferator-activated receptor -regulated gene expression. Proc Natl Acad Sci USA 96:1585–1590[Abstract/Free Full Text]
137. Dowell P, Ishmael J, Avram D, Peterson V, Nevrivy D, Leid M 1997 p300 Functions as a coactivator for the peroxisome proliferator-activated receptor alpha. J Biol Chem 272:33435–33443[Abstract/Free Full Text]
138. Mizukami J, Taniguchi T 1997 The antidiabetic agent thiazolidinedione stimulates the interaction between PPAR and CBP. Biochem Biophys Res Commun 240:61–64[CrossRef][Medline]
139. Schulman IG, Shao G, Heyman RA 1998 Transactivation by retinoid x receptor-peroxisome proliferator-activated receptor (PPAR) heterodimers: intermolecular synergy requires only the PPAR hormone-dependent activation function. Mol Cell Biol 18:3483–3494[Abstract/Free Full Text]
140. Kamei Y, Xu L, Heinzel T, Torchia J, Kurokawa R, Gloss B, Lin S-C, Heyman RA, Rose DW, Glass CK, Rosenfeld MG 1996 A CBP integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors. Cell 85:403–414[CrossRef][Medline]
141. Hanstein B, Eckner R, DiRenzo J, Halachmi S, Liu H, Searcy B, Kurokawa R, Brown M 1996 p300 Is a component of an estrogen receptor coactivator complex. Proc Natl Acad Sci USA 93:11540–11545[Abstract/Free Full Text]
142. Chakravarti D, LaMorte VJ, Nelson MC, Nakajima T, Schulman IG, Juguilon H, Montminy M, Evans RM 1996 Role of CBP/P300 in nuclear receptor signalling. Nature 383:99–103[CrossRef][Medline]
143. Arias J, Alberts AS, Brindle P, Claret FX, Smeal T, Karin M, Feramisco J, Montminy M 1994 Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. Nature 370:226–229[CrossRef][Medline]
144. Kwok RPS, Lundblad JR, Chrivia JC, Richards JP, Bachinger HP, Brennan RG, Roberts SGE, Green MR, Goodman RH 1994 Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature 370:223–226[CrossRef][Medline]
145. Eckner R, Yao TP, Oldread E, Livingston DM 1996 Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. Genes Dev 10:2478–2490[Abstract/Free Full Text]
146. Bhattacharya S, Eckner R, Grossman S, Oldread E, Arany Z, D’Andrea A, Livingston DM 1996 Cooperation of Stat2 and p300/CBP in signalling induced by interferon-. Nature 383:344–347[CrossRef][Medline]
147. Zhang JJ, Vinkemeier U, Gu W, Chakravarti D, Horvath CM, Darnell Jr JE 1996 Two contact regions between Stat1 and CBP/p300 in interferon signaling. Proc Natl Acad Sci USA 93:15092–15096[Abstract/Free Full Text]
148. Horvai AE, Xu L, Korzus E, Brard G, Kalafus D, Mullen TM, Rose DW, Rosenfeld MG, Glass CK 1997 Nuclear integration of JAK/STAT and Ras/AP-1 signaling by CBP and p300. Proc Natl Acad Sci USA 94:1074–1079[Abstract/Free Full Text]
149. Perkins ND, Felzien LK, Betts JC, Leung K, Beach DH, Nabel GJ 1997 Regulation of NF-kappaB by cyclin-dependent kinases associated with the p300 coactivator. Science 275:523–527[Abstract/Free Full Text]
150. Giles RH, Peters DJM, Breuning MH 1998 Conjunction dysfunction: CBP/p300 in human disease. Trends Genet 14:178–183[CrossRef][Medline]
151. Janknecht R, Hunter T 1996 Transcription – a growing coactivator network. Nature 383:22–23[CrossRef][Medline]
152. Shikama N, Lyon J, Lathangue NB 1997 The p300/CBP family: integrating signals with transcription factors and chromatin. Trends Cell Biol 7:230–236
153. Schulman IG, Li C, Schwabe JWR, Evans RM 1997 The phantom ligand effect: allosteric control of transcription by the retinoid X receptor. Genes Dev 11:299–308[Abstract/Free Full Text]
154. Mascrez B, Mark M, Dierich A, Ghyselinck N, Kastner P, Chambon P 1998 The RXR ligand-dependent activation function (AF-2) is important for mouse development. Development 125:4691–4707[Abstract]
155. Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM 1998 A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell 92:829–839[CrossRef][Medline]
156. Zhu Y, Qi C, Jain S, Rao MS, Reddy JK 1997 Isolation and characterization of PBP, a protein that interacts with peroxisome proliferator-activated receptor. J Biol Chem 272:25500–25506[Abstract/Free Full Text]
157. Yuan CX, Ito M, Fondell JD, Fu ZY, Roeder RG 1998 The TRAP220 component of a thyroid hormone receptor-associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion. Proc Natl Acad Sci USA 95:7939–7944[Abstract/Free Full Text]
158. Rachez C, Suldan Z, Ward J, Chang CP, Burakov D, Erdjument-Bromage H, Tempst P, Freedman LP 1998 A novel protein complex that interacts with the vitamin D3 receptor in a ligand-dependent manner and enhances VDR transactivation in a cell-free system. Genes Dev 12:1787–1800[Abstract/Free Full Text]
159. Miyata KS, McCaw SE, Meertens LM, Patel HV, Rachubinski RA, Capone JP 1998 Receptor-interacting protein 140 interacts with and inhibits transactivation by, peroxisome proliferator-activated receptor and liver-X-receptor . Mol Cell Endocrinol 146:69–76[CrossRef][Medline]
160. Treuter E, Albrektsen T, Johansson L, Leers J, Gustafsson JA 1998 A regulatory role for RIP140 in nuclear receptor activation. Mol Endocrinol 12:864–881[Abstract/Free Full Text]
161. Krey G, Mahfoudi A, Wahli W 1995 Functional interactions of peroxisome proliferator-activated receptor, retinoid-X receptor, and Sp1 in the transcriptional regulation of the acyl-coenzyme-A oxidase promoter. Mol Endocrinol 9:219–231[Abstract/Free Full Text]
162. Bannister AJ, Kouzarides T 1996 The CBP co-activator is a histone acetyltransferase. Nature 384:641–643[CrossRef][Medline]
163. Ogryzko VV, Schiltz RL, Russanova V, Howard BH, Nakatani Y 1996 The transcriptional coactivators p300 and CBP are histone acetyltransferases. Cell 87:953–959[CrossRef][Medline]
164. Martinez-Balbas MA, Bannister AJ, Martin K, Haus-Seuffert P, Meisterernst M, Kouzarides T 1998 The acetyltransferase activity of CBP stimulates transcription. EMBO J 17:2886–2893[CrossRef][Medline]
165. Cullingford TE, Bhakoo K, Peuchen S, Dolphin CT, Patel R, Clark JB 1998 Distribution of mRNAs encoding the peroxisome proliferator-activated receptor , ß, and and the retinoid X receptor , ß, and in rat central nervous system. J Neurochem 70:1366–1375[Medline]
166. Braissant O, Wahli W 1998 Differential expression of peroxisome proliferator-activated receptor-, -ß, and - during rat embryonic development. Endocrinology 139:2748–2754[Abstract/Free Full Text]
167. Braissant O, Foufelle F, Scotto C, Dauça M, Wahli W 1996 Differential expression of peroxisome proliferator-activated receptors: tissue distribution of PPAR-, -ß and - in the adult rat. Endocrinology 137:354–366[Abstract]
168. Lemberger T, Braissant O, Juge-Aubry C, Keller H, Saladin R, Staels B, Auwerx J, Burger AG, Meier CA, Wahli W 1996 PPAR tissue distribution and interactions with other hormone-signaling pathways. Ann NY Acad Sci 804:231–251[Medline]
169. Palmer CNA, Hsu M-H, Griffin KJ, Raucy JL, Johnson EF 1998 Peroxisome proliferator activated receptor- expression in human liver. Mol Pharmacol 53:14–22[Abstract/Free Full Text]
170. Mukherjee R, Jow L, Croston GE, Paterniti Jr JR 1997 Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPAR2 versus PPAR1 and activation with retinoid X receptor agonists and antagonists. J Biol Chem 272:8071–8076[Abstract/Free Full Text]
171. Auboeuf D, Rieusset J, Fajas L, Vallier P, Frering V, Riou JP, Staels B, Auwerx J, Laville M, Vidal H 1997 Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor- in humans: no alteration in adipose tissue of obese and NIDDM patients. Diabetes 48:1319–1327
172. Steineger HH, Sorensen HN, Tugwood JD, Skrede S, Spydevold O, Gautvik KM 1994 Dexamethasone and insulin demonstrate marked and opposite regulation of the steady-state mRNA level of the peroxisomal proliferator-activated receptor (PPAR) in hepatic cells – hormonal modulation of fatty-acid-induced transcription. Eur J Biochem 225:967–974[Medline]
173. Lemberger T, Staels B, Saladin R, Desvergne B, Auwerx J, Wahli W 1994 Regulation of the peroxisome proliferator-activated receptor gene by glucocorticoids. J Biol Chem 269:24527–24530[Abstract/Free Full Text]
174. Lemberger T, Saladin R, Vazquez M, Assimacopoulos F, Staels B, Desvergne B, Wahli W, Auwerx J 1996 Expression of the peroxisome proliferator-activated receptor gene is stimulated by stress and follows a diurnal rhythm. J Biol Chem 271:1764–1769[Abstract/Free Full Text]
175. Lemberger T, Desvergne B, Wahli W 1996 Peroxisome proliferator-activated receptors: a nuclear receptor signaling pathway in lipid physiology. Annu Rev Cell Dev Biol 12:335–363[CrossRef][Medline]
176. Yamada J, Sugiyama H, Watanabe T, Suga T 1995 Suppressive effect of growth hormone on the expression of peroxisome proliferator-activated receptor in cultured rat hepatocytes. Res Commun Mol Pathol Pharmacol 90:173–176[Medline]
177. Wan YJ, Morimoto M, Thurman RG, Bojes HK, French SW 1995 Expression of the peroxisome proliferator-activated receptor gene is decreased in experimental alcoholic liver disease. Life Sci 56:307–317[CrossRef][Medline]
178. Sterchele PF, Sun H, Peterson RE, Vanden Heuvel JP 1996 Regulation of peroxisome proliferator-activated receptor mRNA in rat liver. Arch Biochem Biophys 326:281–289[CrossRef][Medline]
179. Zhou YT, Shimabukuro M, Wang MY, Lee Y, Higa M, Milburn JL, Newgard CB, Unger RH 1998 Role of peroxisome proliferator-activated receptor in disease of pancreatic ß cells. Proc Natl Acad Sci USA 95:8898–8903[Abstract/Free Full Text]
180. Dreyer C, Keller H, Mahfoudi A, Laudet V, Krey G, Wahli W 1993 Positive regulation of the peroxisomal ß-oxydation pathway by fatty acids through activation of peroxisome proliferator-activated receptors (PPAR). Biol Cell 77:67–76[CrossRef][Medline]
181. Granneman J, Skoff R, Yang X 1998 Member of the peroxisome proliferator-activated receptor family of transcription factors is differentially expressed by oligodendrocytes. J Neurosci Res 51:563–573[CrossRef][Medline]
182. Lim H, Gupta RA, Ma WG, Paria BC, Moller D, Morrow JD, DuBois RN, Trzaskos JM, Dey SK 1999 Cyclo-oxygenase-2-derived prostacyclin mediates embryo implantation in the mouse via PPAR. Genes Dev 13:1561–1574[Abstract/Free Full Text]
183. Tontonoz P, Hu E, Graves RA, Budavari AI, Spiegelman BM 1994 mPPAR2: tissue-specific regulator of an adipocyte enhancer. Genes Dev 8:1224–1234[Abstract/Free Full Text]
184. Mansen A, Guardiola-Diaz H, Rafter J, Branting C, Gustafsson JA 1996 Expression of the peroxisome proliferator-activated receptor (PPAR) in the mouse colonic mucosa. Biochem Biophys Res Commun 222:844–851[CrossRef][Medline]
185. Lefebvre AM, Chen I, Desreumaux P, Najib J, Fruchart JC, Geboes K, Briggs M, Heyman R, Auwerx J 1998 Activation of the peroxisome proliferator-activated receptor promotes the development of colon tumors in C57BL/6J-APC^Min/+ mice. Nat Med 4:1053–1057[CrossRef][Medline]
186. Saez E, Tontonoz P, Nelson MC, Alvarez JGA, Ming UT, Baird S, Thomazy VA, Evans RM 1998 Activators of the nuclear receptor PPAR enhance colon polyp formation. Nat Med 4:1058–1061[CrossRef][Medline]
187. Vidal-Puig AJ, Considine RV, Jimenez-Linan M, Werman A, Pories WJ, Caro JF, Flier JS 1997 Peroxisome proliferator-activated receptor gene expression in human tissues. Effects of obesity, weight loss, and regulation by insulin and glucocorticoids. J Clin Invest 99:2416–2422[Medline]
188. Xing H, Northrop JP, Grove JR, Kilpatrick KE, Su JL, Ringold GM 1997 TNF-mediated inhibition and reversal of adipocyte differentiation is accompanied by suppressed expression of PPAR without effects on Pref-1 expression. Endocrinology 138:2776–2783[Abstract/Free Full Text]
189. Hill M, Young M, McCurdy C, Gimble J 1997 Decreased expression of murine PPAR in adipose tissue during endotoxemia. Endocrinology 138:3073–3076[Abstract/Free Full Text]
190. Rousseau V, Becker DJ, Ongemba LN, Rahier J, Henquin JC, Brichard SM 1997 Developmental and nutritional changes of ob and PPAR2 gene expression in rat white adipose tissue. Biochem J 321:451–456
191. Pearson SL, Cawthorne MA, Clapham JC, Dunmore SJ, Holmes SD, Moore GB, Smith SA, Tadayyon M 1996 The thiazolidinedione insulin sensitiser, BRL 49653, increases the expression of PPAR and aP2 in adipose tissue of high-fat-fed rats. Biochem Biophys Res Commun 229:752–757[CrossRef][Medline]
192. Shimoike T, Yanase T, Umeda F, Ichino I, Takayanagi R, Nawata H 1998 Subcutaneous or visceral adipose tissue expression of the PPARgamma gene is not altered in the fatty (fa/fa) Zucker rat. Metabolism 47:1494–1498[CrossRef][Medline]
193. Vidal-Puig A, Jimenez-Linan M, Lowell BB, Hamann A, Hu E, Spiegelman B, Flier JS, Moller DE 1996 Regulation of PPAR gene expression by nutrition and obesity in rodents. J Clin Invest 97:2553–2561[Medline]
194. Hotta K, Gustafson TA, Yoshioka S, Ortmeyer HK, Bodkin NL, Hansen BC 1998 Relationships of PPAR and PPAR2 mRNA levels to obesity, diabetes and hyperinsulinaemia in rhesus monkeys. Int J Obes Relat Metab Disord 22:1000–1010[CrossRef][Medline]
195. Rieusset J, Andreelli F, Aubeuf D, Roques M, Vallier P, Riou J, Auwerx J, Laville M, Vidal H 1999 Insulin acutely regulates the expression of the peroxisome proliferator-activated receptor- in human adipocytes. Diabetes 48:699–705[Abstract]
196. Kruszynska YT, Mukherjee R, Jow L, Dana S, Paterniti Jr JR, Olefsky JM 1998 Skeletal muscle peroxisome proliferator-activated receptor expression in obesity and non-insulin-dependent diabetes mellitus. J Clin Invest 101:543–548[Medline]
197. Yen CJ, Beamer BA, Negri C, Silver K, Brown KA, Yarnall DP, Burns DK, Roth J, Shuldiner AR 1997 Molecular scanning of the human peroxisome proliferator activated receptor (hPPAR) gene in diabetic Caucasians: identification of a Pro12Ala PPAR 2 missense mutation. Biochem Biophys Res Commun 241:270–274[CrossRef][Medline]
198. Deeb SS, Fajas L, Nemoto M, Pihlajamäki J, Mykkänen L, Kuusisto J, Laakso M, Fujimoto W, Auwerx J 1998 A Pro12Ala substitution in PPAR 2 associated with decreased receptor activity, lower body mass index and improved insulin sensitivity. Nat Genet 20:284–287[CrossRef][Medline]
199. Ringel J, Engeli S, Distler A, Sharma A 1999 Pro12Ala missense mutation of the peroxisome proliferator-activated receptor and diabetes mellitus. Biochem Biophys Res Commun 254:450–453[CrossRef][Medline]
200. Mori Y, Kim-Motoyama H, Katakura T, Yasuda K, Kadowaki H, Beamer BA, Shuldiner AR, Akanuma Y, Yazaki Y, Kadowaki T 1998 Effect of the Pro12Ala variant of the human peroxisome proliferator-activated receptor gamma 2 gene on adiposity, fat distribution, and insulin sensitivity in Japanese men. Biochem Biophys Res Commun 251:195–198[CrossRef][Medline]
201. Vigouroux C, Fajas L, Khallouf E, Meier M, Gyapay G, Lascols O, Auwerx J, Weissenbach J, Capeau J, Magre J 1998 Human peroxisome proliferator-activated receptor-gamma2: genetic mapping, identification of a variant in the coding sequence, and exclusion as the gene responsible for lipoatrophic diabetes. Diabetes 47:490–492[Medline]
202. Beamer BA, Yen CJ, Andersen RE, Muller D, Elahi D, Cheskin LJ, Andres R, Roth J, Shuldiner AR 1998 Association of the Pro12Ala variant in the peroxisome proliferator-activated receptor-gamma2 gene with obesity in two Caucasian populations. Diabetes 47:1806–1808[Medline]
203. Hanis CL, Boerwinkle E, Chakraborty R, Ellsworth DL, Con- cannon P, Stirling B, Morrison VA, Wapelhorst B, Spielman RS, Gogolin-Ewens KJ, Shepard JM, Williams SR, Risch N, Hinds D, Iwasaki N, Ogata M, Omori Y, Petzold C, Rietzch H, Schroder HE, Schulze J, Cox NJ, Menzel S, Boriraj VV, Chen X, Lim LR, Linder T, Mereu LE, Wang Y-Q, Xiang K, Yamagata K, Yang Y, Bell GI 1996 A genome-wide search for human non-insulin-dependent (type 2) diabetes genes reveals a major susceptibility locus on chromosome 2. Nat Genet 13:161–166[CrossRef][Medline]
204. Norman RA, Thompson DB, Foroud T, Garvey WT, Bennett PH, Bogardus C, Ravussin E 1997 Genome wide search for genes influencing percent body fat in Pima Indians: suggestive linkage at chromosome 11q21–q22. Pima Diabetes Gene Group. Am J Hum Genet 60:166–173[Medline]
205. Suruga K, Mochizuki K, Kitagawa M, Goda T, Horie N, Takeishi K, Takase S 1999 Transcriptional regulation of cellular retinol-binding protein, type II gene expression in small intestine by dietary fat. Arch Biochem Biophys 362:159–166[CrossRef][Medline]
206. Poirier H, Niot I, Degrace P, Monnot MC, Bernard A, Besnard P 1997 Fatty acid regulation of fatty acid-binding protein expression in the small intestine. Am J Physiol 273:G289–295
207. Mallordy A, Poirier H, Besnard P, Niot I, Carlier H 1995 Evidence for transcriptional induction of the liver fatty-acid-binding-protein gene by bezafibrate in the small intestine. Eur J Biochem 227:801–807[Medline]
208. Bass NM, Manning JA, Ockner RK, Gordon JI, Seetharam S, Alpers DH 1985 Regulation of the biosynthesis of two distinct fatty acid-binding proteins in rat liver and intestine. Influences of sex difference and of clofibrate. J Biol Chem 260:1432–1436[Abstract/Free Full Text]
209. Poirier H, Degrace P, Niot I, Bernard A, Besnard P 1996 Localization and regulation of the putative membrane fatty-acid transporter (FAT) in the small intestine. Comparison with fatty acid-binding proteins (FABP). Eur J Biochem 238:368–373[Medline]
210. Rigotti A, Trigatti B, Babitt J, Penman M, Xu S, Krieger M 1997 Scavenger receptor BI: a cell surface receptor for high density lipoprotein. Curr Opin Lipidol 8:181–188[CrossRef][Medline]
211. Fruchart JC, Brewer HB, Leitersdorf E 1998 Consensus for the use of fibrates in the treatment of dyslipoproteinemia and coronary heart disease. Fibrate Consensus Group. Am J Cardiol 81:912–917[CrossRef][Medline]
212. Ito Y, Azrolan N, O’Connell A, Walsh A, Breslow JL 1990 Hypertriglyceridemia as a result of human apo CIII gene expression in transgenic mice. Science 249:790–793[Abstract/Free Full Text]
213. Vu-Dac N, Chopin-Delannoy S, Gervois P, Bonnelye E, Martin G, Fruchart JC, Laudet V, Staels B 1998 The nuclear receptors peroxisome proliferator-activated receptor alpha and Rev-erbalpha mediate the species-specific regulation of apolipoprotein A-I expression by fibrates. J Biol Chem 273:25713–25720[Abstract/Free Full Text]
214. Widom RL, Rhee M, Karathanasis SK 1992 Repression by ARP-1 sensitizes apolipoprotein-AI gene responsiveness to RXR and retinoic acid. Mol Cell Biol 12:3380–3389[Abstract/Free Full Text]
215. Ladias JA, Hadzopoulou-Cladaras M, Kardassis D, Cardot P, Cheng J, Zannis V, Cladaras C 1992 Transcriptional regulation of human apolipoprotein genes ApoB, ApoCIII, and ApoAII by members of the steroid hormone receptor superfamily HNF-4, ARP-1, EAR-2, and EAR-3. J Biol Chem 267:15849–15860[Abstract/Free Full Text]
216. Cardot P, Chambaz J, Kardassis D, Cladaras C, Zannis VI 1993 Factors participating in the liver-specific expression of the human apolipoprotein A-II gene and their significance for transcription. Biochemistry 32:9080–9093[CrossRef][Medline]
217. Schoonjans K, Staels B, Auwerx J 1996 Role of the peroxisome proliferator-activated receptor (PPAR) in mediating the effects of fibrates and fatty acids on gene expression. J Lipid Res 37:907–925[Abstract]
218. Erdman D, Heim J 1995 Orphan nuclear receptor HNF-4 binds to the human coagulation factor VII promoter. J Biol Chem 270:22988–22996[Abstract/Free Full Text]
219. Tian J, Schibler U 1991 Tissue-specific expression of the gene encoding hepatocyte nuclear factor-1 may involve hepatocyte nuclear factor-4. Genes Dev 5:2225–2234[Abstract/Free Full Text]
220. Leibowitz MD, Berger JB 1997 Method for raising HDL cholesterol level. Patent CO7D 307/79, A61K 31/34; WO 97/28149
221. Martin G, Schoonjans K, Lefebvre AM, Staels B, Auwerx J 1997 Coordinate regulation of the expression of the fatty acid transport protein and acyl-CoA synthetase genes by PPAR and PPAR activators. J Biol Chem 272:28210–28217[Abstract/Free Full Text]
222. Motojima K, Passilly P, Peters JM, Gonzalez FJ, Latruffe N 1998 Expression of putative fatty acid transporter genes are regulated by peroxisome proliferator-activated receptor and activators in a tissue- and inducer-specific manner. J Biol Chem 273:16710–16714[Abstract/Free Full Text]
223. Schoonjans K, Watanabe M, Suzuki H, Mahfoudi A, Krey G, Wahli W, Grimaldi P, Staels B, Yamamoto T, Auwerx J 1995 Induction of the acyl-coenzyme A synthetase gene by fibrates and fatty acids is mediated by a peroxisome proliferator response element in the C promoter. J Biol Chem 270:19269–19276[Abstract/Free Full Text]
224. Lock EA, Mitchell AM, Elcombe CR 1989 Biochemical mechanisms of induction of hepatic peroxisome proliferation. Annu Rev Pharmacol Toxicol 29:145–163[CrossRef][Medline]
225. Lee SS, Pineau T, Drago J, Lee EJ, Owens JW, Kroetz DL, Fernandez-Salguero PM, Westphal H, Gonzalez FJ 1995 Targeted disruption of the isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators. Mol Cell Biol 15:3012–3022[Abstract]
226. Marcus SL, Miyata KS, Zhang BW, Subramani S, Rachubinski RA, Capone JP 1993 Diverse peroxisome proliferator-activated receptors bind to the peroxisome proliferator-responsive elements of the rat hydratase/dehydrogenase and fatty acyl-CoA oxidase genes but differentially induce expression. Proc Natl Acad Sci USA 90:5723–5727[Abstract/Free Full Text]
227. Zhang BW, Marcus SL, Sajjadi FG, Alvares K, Reddy JK, Subramani S, Rachubinski RA, Capone JP 1992 Identification of a peroxisome proliferator-responsive element upstream of the gene encoding rat peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase. Proc Natl Acad Sci USA 89:7541–7545[Abstract/Free Full Text]
228. Mannaerts GP, Van Veldhoven PP 1993 Metabolic role of mammalian peroxisomes. In: Gibson G, Lake B (eds) Peroxisomes: Biology and Importance in Toxicology and Medicine. Taylor & Francis, London, pp 19–62
229. Sprecher H, Luthria DL, Mohammed BS, Baykousheva SP 1995 Reevaluation of the pathways for the biosynthesis of polyunsaturated fatty acids. J Lipid Res 36:2471–2477[Abstract]
230. Lee W-NP, Lim S, Bassilian S, Bergner EA, Edmond J 1998 Fatty acid cycling in human hepatoma cells and the effects of troglitazone. J Biol Chem 273:20929–20934[Abstract/Free Full Text]
231. Brady PS, Marine KA, Brady LJ, Ramsay RR 1989 Co-ordinate induction of hepatic mitochondrial and peroxisomal carnitine acyltransferase synthesis by diet and drugs. Biochem J 260:93–100[Medline]
232. Foxworthy PS, Perry DN, Hoover DM, Eacho PI 1990 Changes in hepatic lipid metabolism associated with lipid accumulation and its reversal in rats given the peroxisome proliferator LY171883. Toxicol Appl Pharmacol 106:375–383[CrossRef][Medline]
233. Yu GS, Lu YC, Gulick T 1998 Co-regulation of tissue-specific alternative human carnitine palmitoyltransferase Iß gene promoters by fatty acid enzyme substrate. J Biol Chem 273:32901–32909[Abstract/Free Full Text]
234. Mascaro C, Acosta E, Ortiz JA, Marrero PF, Hegardt FG, Haro D 1998 Control of human muscle-type carnitine palmitoyltransferase I gene transcription by peroxisome proliferator-activated receptor. J Biol Chem 273:8560–8563[Abstract/Free Full Text]
235. Brandt JM, Djouadi F, Kelly DP 1998 Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor . J Biol Chem 273:23786–23792[Abstract/Free Full Text]
236. Gulick T, Cresci S, Caira T, Moore DD, Kelly DP 1994 The peroxisome proliferator-activated receptor regulates mitochondrial fatty acid oxidative enzyme gene expression. Proc Natl Acad Sci USA 91:11012–11016[Abstract/Free Full Text]
237. Rodriguez JC, Gil-Gomez G, Hegardt FG, Haro D 1994 Peroxisome proliferator-activated receptor mediates induction of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by fatty acids. J Biol Chem 269:18767–18772[Abstract/Free Full Text]
238. Meertens LM, Miyata KS, Cechetto JD, Rachubinski RA, Capone JP 1998 A mitochondrial ketogenic enzyme regulates its gene expression by association with the nuclear receptor PPAR. EMBO J 17:6972–6978[CrossRef][Medline]
239. Aoyama T, Peters JM, Iritani N, Nakajima T, Furihata K, Hashimoto T, Gonzalez FJ 1998 Altered constitutive expression of fatty acid-metabolizing enzymes in mice lacking the peroxisome proliferator-activated receptor (PPAR). J Biol Chem 273:5678–5684[Abstract/Free Full Text]
240. Kersten S, Seydoux J, Peters JM, Gonzalez FJ, Desvergne B, Wahli W 1999 Peroxisome proliferator-activated receptor mediates the adaptive response to fasting. J Clin Invest 103:1489–1498[Medline]
241. Leone T, Weinheimer C, Kelly D 1999 A critical role for the peroxisome proliferator-activated receptor (PPAR) in the cellular fasting response: the PPAR-null mouse as a model of fatty acid oxidation disorders. Proc Natl Acad Sci USA 96:7473–7478[Abstract/Free Full Text]
242. Tontonoz P, Hu E, Devine J, Beale EG, Spiegelman BM 1995 PPAR2 regulates adipose expression of the phosphoenolpyruvate carboxykinase gene. Mol Cell Biol 15:351–357[Abstract]
243. Djouadi F, Weinheimer CJ, Saffitz JE, Pitchford C, Bastin J, Gonzales FJ, Kelly DP 1998 A gender-related defect in lipid metabolism and glucose homeostasis in peroxisome proliferator-activated receptor -deficient mice. J Clin Invest 102:1083–1091[Medline]
244. Costet P, Legendre C, Moré J, Edgar A, Galtier P, Pineau T 1998 Peroxisome proliferator-activated receptor -isoform deficiency leads to progressive dyslipidemia with sexually dismorphic obesity and steatosis. J Biol Chem 273:29577–29585[Abstract/Free Full Text]
245. Ma H, Sprecher HW, Kolattukudy PE 1998 Estrogen-induced production of a peroxisome proliferator-activated receptor (PPAR) ligand in a PPAR-expressing tissue. J Biol Chem 273:30131–30138[Abstract/Free Full Text]
246. Castelein H, Gulick T, Declercq EP, Mannaerts GP, Moore DD, Baes MI 1994 The peroxisome proliferator activated receptor regulates malic enzyme gene expression. J Biol Chem 269:26754–26758[Abstract/Free Full Text]
247. Toussant MJ, Wilson MD, Clarke SD 1981 Coordinate suppression of liver acetyl-CoA carboxylase and fatty acid synthetase by polyunsaturated fat. J Nutr 111:146–153
248. Clarke SD, Turini M, Jump D 1997 Polyunsaturated fatty acids regulate lipogenic and peroxisomal gene expression by independent mechanisms. Prostaglandins Leukot Essent Fatty Acids 57:65–69[CrossRef][Medline]
249. Miller CW, Ntambi JM 1996 Peroxisome proliferators induce mouse liver stearoyl-CoA desaturase 1 gene expression. Proc Natl Acad Sci USA 93:9443–9448[Abstract/Free Full Text]
250. Belinski SA, Kari FW, Kauffman FC, Thurman RG 1987 Effect of beta-naphthoflavone on mitochondrial supply of reducing equivalents for monooxygenation in periportal and pericentral regions of the liver lobule. Mol Pharmacol 32:315–320[Abstract]
251. Ren B, Thelen AP, Peters JM, Gonzalez FJ, Jump DB 1997 Polyunsaturated fatty acid suppression of hepatic fatty acid synthase and S14 gene expression does not require peroxisome proliferator-activated receptor . J Biol Chem 272:26827–26832[Abstract/Free Full Text]
252. Hertz R, Magenheim J, Berman I, Bar-Tana J 1998 Fatty acyl-CoA thioesters are ligands of hepatic nuclear factor-4-. Nature 392:512–516[CrossRef][Medline]
253. Jedlitschky G, Mayatepek E, Keppler D 1993 Peroxisomal leukotriene degradation: biochemical and clinical implications. Adv Enzyme Regul 33:181–194[CrossRef][Medline]
254. Muerhoff AS, Griffin KJ, Johnson EF 1992 The peroxisome proliferator-activated receptor mediates the induction of CYP4A6, a cytochrome-P450 fatty-acid -hydroxylase, by clofibric acid. J Biol Chem 267:19051–19053[Abstract/Free Full Text]
255. Aldridge TC, Tugwood JD, Green S 1995 Identification and characterization of DNA elements implicated in the regulation of CYP4A1 transcription. Biochem J 306:473–479
256. Kroetz DL, Yook P, Costet P, Bianchi P, Pineau T 1998 Peroxisome proliferator-activated receptor controls the hepatic CYP4A induction adaptive response to starvation and diabetes. J Biol Chem 273:31581–31589[Abstract/Free Full Text]
257. Tontonoz P, Hu E, Spiegelman BM 1994 Stimulation of adipogenesis in fibroblasts by PPAR2, a lipid-activated transcription factor. Cell 79:1147–1156[CrossRef][Medline]
258. Kletzien RF, Foellmi LA, Harris PK, Wyse BM, Clarke SD 1992 Adipocyte fatty acid-binding protein: regulation of gene expression in vivo and in vitro by an insulin-sensitizing agent. Mol Pharmacol 42:558–562[Abstract]
259. Sandouk T, Reda D, Hofmann C 1993 Antidiabetic agent pioglitazone enhances adipocyte differentiation of 3T3–F442A cells. Am J Physiol 264:C1600–1608
260. Chawla A, Schwarz EJ, Dimaculangan DD, Lazar MA 1994 Peroxisome proliferator-activated receptor (PPAR) gamma: adipose-predominant expression and induction early in adipocyte differentiation. Endocrinology 135:798–800[Abstract]
261. Chen H, Jackson S, Doro M, McGowan S 1998 Perinatal expression of genes that may participate in lipid metabolism by lipid-laden lung fibroblasts. J Lipid Res 39:2483–2492[Abstract/Free Full Text]
262. Mandrup S, Lane MD 1997 Regulating adipogenesis. J Biol Chem 272:5367–5370[Free Full Text]
263. Wu Z, Xie Y, Bucher NLR, Farmer SR 1995 Conditional ectopic expression of C/EBPß in NIH-3T3 cells induces PPAR and stimulates adipogenesis. Genes Dev 9:2350–2363[Abstract/Free Full Text]
264. Clarke SL, Robinson CE, Gimble JM 1997 CAAT/enhancer binding proteins directly modulate transcription from the peroxisome proliferator-activated receptor 2 promoter. Biochem Biophys Res Commun 240:99–103[CrossRef][Medline]
265. El-Jack AK, Hamm JK, Pilch PF, Farmer SR 1999 Reconstitution of insulin-sensitive glucose transport in fibroblasts requires expression of both PPAR and C/EBP. J Biol Chem 274:7946–7951[Abstract/Free Full Text]
266. Adelmant G, Gilbert JD, Freytag SO 1998 Human translocation liposarcoma-CCAAT/enhancer binding protein (C/EBP) homologous protein (TLS-CHOP) oncoprotein prevents adipocyte differentiation by directly interfering with C/EBPß function. J Biol Chem 273:15574–15581[Abstract/Free Full Text]
267. Wang N-D, Finegold MJ, Bradley A, Ou CN, Abdelsayed SV, Wilde MD, Ranee Taylor L, Wilson DR, Darlington GJ 1995 Impaired energy homeostasis in C/EBP knockout mice. Science 269:1108–1112[Abstract/Free Full Text]
268. Tanaka T, Yoshida N, Kishimoto T, Akira S 1997 Defective adipocyte differentiation in mice lacking the C/EBPß and/or C/EBP gene. EMBO J 16:7432–7443[CrossRef][Medline]
269. Moitra J, Mason MM, Olive M, Krylov D, Gavrilova O, Marcus-Samuels B, Feigenbaum L, Lee E, Aoyama T, Eckhaus M, Reitman ML, Vinson C 1998 Life without white fat: a transgenic mouse. Genes Dev 12:3168–3181[Abstract/Free Full Text]
270. Wu Z, Bucher NLR, Farmer SR 1996 Induction of peroxisome proliferator-activated receptor during the conversion of 3T3 fibroblasts into adipocytes is mediated by C/EBPß, C/EBP, and glucocorticoids. Mol Cell Biol 16:4128–4136[Abstract]
271. Tontonoz P, Kim JB, Graves RA, Spiegelman BM 1993 ADD1: a novel helix-loop-helix transcription factor associated with adipocyte determination and differentiation. Mol Cell Biol 13:4753–4759[Abstract/Free Full Text]
272. Kim JB, Wright HM, Wright M, Spiegelman BM 1998 Add1/SREBP1 activates PPAR through the production of endogenous ligand. Proc Natl Acad Sci USA 95:4333–4337[Abstract/Free Full Text]
273. Shimomura I, Hammer RE, Richardson JA, Ikemoto S, Bashmakov Y, Goldstein JL, Brown MS 1998 Insulin resistance and diabetes mellitus in transgenic mice expressing nuclear SREBP-1c in adipose tissue: model for congenital generalized lipodystrophy. Genes Dev 12:3182–3194[Abstract/Free Full Text]
274. Fajas L, Schoonjans K, Gelman L, Kim J, Najib J, Martin G, Fruchart J-C, Brigg M, Spiegelman B, Auwerx J 1999 Regulation of peroxisome proliferator-activated receptor expression by adipocyte differentiation and determination factor 1/sterol regulatory element binding protein 1: implications for adipocyte differentiation and metabolism. Mol Cell Biol 19:5495–5503[Abstract/Free Full Text]
275. Brun RP, Tontonoz P, Forman BM, Ellis R, Chen J, Evans RM, Spiegelman BM 1996 Differential activation of adipogenesis by multiple PPAR isoforms. Genes Dev 10:974–984[Abstract/Free Full Text]
276. Bastie C, Holst D, Gaillard D, Jehl-Pietri C, Grimaldi P 1999 Expression of peroxisome proliferator-activated receptor PPAR promtes induction of PPAR and adipocyte differentiation in 3T3C2 fibroblasts. J Biol Chem 274:21920–21925[Abstract/Free Full Text]
277. Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, Ailhaud G 1997 Differentiation of embryonic stem cells into adipocytes in vitro. J Cell Sci 110:1279–1285[Abstract]
278. Hu E, Tontonoz P, Spiegelman BM 1995 Transdifferentiation of myoblasts by the adipogenic transcription factors PPAR and C/EBP. Proc Natl Acad Sci USA 92:9856–9860[Abstract/Free Full Text]
279. Teboul L, Gaillard D, Staccini L, Inadera H, Amri EZ, Grimaldi PA 1995 Thiazolidinediones and fatty acids convert myogenic cells into adipose-like cells. J Biol Chem 270:28183–28187[Abstract/Free Full Text]
280. Grimaldi PA, Teboul L, Inadera H, Gaillard D, Amri EZ 1997 Trans-differentiation of myoblasts to adipoblasts: triggering effects of fatty acids and thiazolidinediones. Prostaglandins Leukot Essent Fatty Acids 57:71–75[CrossRef][Medline]
281. Gimble JM, Robinson CEE, Wu X, Kelly KA, Rodriguez BR, Kliewer SA, Lehmann JM, Morris DC 1996 Peroxisome proliferator-activated receptor activation by thiazolidinediones induces adipogenesis in bone marrow stromal cells. Mol Pharmacol 50:1087–1094[Abstract]
282. Deldar A, Williams G, Stevens C 1993 Pathogenesis of thiazolidinedione induced hematoxicity in the dog. Diabetes [Suppl] 42:179
283. Schoonjans K, Peinado-Onsurbe AM, Heyman RA, Briggs M, Deeb S, Staels B, Auwerx J 1996 PPAR and PPAR activators direct a distinct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene. EMBO J 15:5336–5348[Medline]
284. Frohnert BI, Hui TY, Bernlohr DA 1999 Identification of a functional peroxisome proliferator-activated responsive element in the murine fatty acid transport protein gene. J Biol Chem 274:3970–3977[Abstract/Free Full Text]
285. Devine J, Eubank D, Clouthier D, Tontonoz P, Spiegelman B, Hammer R, Beale E 1999 Adipose expression of the phosphoenolpyruvate carboxykinase promoter requires peroxisome proliferator-activated receptor gamma and 9-cis-retinoic acid receptor binding to an adipocyte-specific enhancer in vivo. J Biol Chem 274:13604–13612[Abstract/Free Full Text]
286. Wu Z, Xie Y, Morrison RF, Bucher NL, Farmer SR 1998 PPAR induces the insulin-dependent glucose transporter GLUT4 in the absence of C/EBP during the conversion of 3T3 fibroblasts into adipocytes. J Clin Invest 101:22–32[Medline]
287. Ribon V, Johnson JH, Camp HS, Saltiel AR 1998 Thiazolidinediones and insulin resistance: peroxisome proliferator activated receptor activation stimulates expression of the CAP gene. Proc Natl Acad Sci USA 95:14751–14756[Abstract/Free Full Text]
288. Ribon V, Printen JA, Hoffman NG, Kay BK, Saltiel AR 1998 A novel, multifunctional c-Cbl binding protein in insulin receptor signaling in 3T3–L1 adipocytes. Mol Cell Biol 18:872–879[Abstract/Free Full Text]
289. Martin G, Schoonjans K, Staels B, Auwerx J 1998 PPARgamma activators improve glucose homeostasis by stimulating fatty acid uptake in the adipocytes. Atherosclerosis 137[Suppl]:75–80
290. Lowell BB, S-Susulic V, Hamann A, Lawitts JA, Himms-Hagen J, Boyer BB, Kozak LP, Flier JS 1993 Development of obesity in transgenic mice after genetic ablation of brown adipose tissue. Nature 366:740–742[CrossRef][Medline]
291. Boss O, Samec S, Kühne F, Bijlenga P, Assimacopoulos-Jeannet F, Seydoux J, Giacobino JP, Muzzin P 1998 Uncoupling protein-3 expression in rodent skeletal muscle is modulated by food intake but not by changes in environmental temperature. J Biol Chem 273:5–8[Abstract/Free Full Text]
292. Fleury C, Neverova M, Collins S, Raimbault S, Champigny O, Levi-Meyrueis C, Bouillaud F, Seldin MF, Surwit RS, Ricquier D, Warden CH 1997 Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat Genet 15:269–272[CrossRef][Medline]
293. Boss O, Samec S, Paoloni-Giacobino A, Rossier C, Dulloo A, Seydoux J, Muzzin P, Giacobino JP 1997 Uncoupling protein-3: a new member of the mitochondrial carrier family with tissue-specific expression. FEBS Lett 408:39–42[CrossRef][Medline]
294. Gong DW, He Y, Karas M, Reitman M 1997 Uncoupling protein-3 is a mediator of thermogenesis regulated by thyroid hormone, ß3-adrenergic agonists, and leptin. J Biol Chem 272:24129–24132[Abstract/Free Full Text]
295. Samec S, Seydoux J, Dulloo AG 1998 Role of UCP homologues in skeletal muscles and brown adipose tissue: mediators of thermogenesis or regulators of lipids as fuel substrate? FASEB J 12:715–724[Abstract/Free Full Text]
296. Enerback S, Jacobsson A, Simpson EM, Guerra C, Yamashita H, Harper ME, Kozak LP 1997 Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. Nature 387:90–94[CrossRef][Medline]
297. Tai T, Jennermann C, Brown KK, Oliver BB, MacGinnitie MA, Wilkison WO, Brown HR, Lehmann JM, Kliewer SA, Morris DC, Graves RA 1996 Activation of the nuclear receptor peroxisome proliferator-activated promotes brown adipocyte differentiation. J Biol Chem 271:29909–29914[Abstract/Free Full Text]
298. Sears IB, MacGinnitie MA, Kovacs LG, Graves RA 1996 Differentiation-dependent expression of the brown adipocyte uncoupling protein gene: regulation by peroxisome proliferator-activated receptor . Mol Cell Biol 16:3410–3419[Abstract]
299. Kelly LJ, Vicario PP, Thompson GM, Candelore MR, Doebber TW, Ventre J, Wu MS, Meurer R, Forrest MJ, Conner MW, Cascieri MA, Moller DE 1998 Peroxisome proliferator-activated receptors and mediate in vivo regulation of uncoupling protein (UCP-1, UCP-2, UCP-3) gene expression. Endocrinology 139:4920–4927[Abstract/Free Full Text]
300. Brun S, Carmona M, Mampel T, Vinas O, Giralt M, Iglesias R, Villaroya F 1999 Activators of peroxisome proliferator-activated receptor- induce the expression of the uncoupling protein-3 gene in skeletal muscle: a potential mechanism for the lipid intake-dependent activation of uncoupling protein-3 gene expression at birth. Diabetes 48:1217–1222[Abstract]
301. Guerra C, Koza RA, Walsh K, Kurtz DM, Wood PA, Kozak LP 1998 Abnormal nonshivering thermogenesis in mice with inherited defects of fatty acid oxidation. J Clin Invest 102:1724–1731[Medline]
302. Spiegelman BM, Flier JS 1996 Adipogenesis and obesity: rounding out the big picture. Cell 87:377–389[CrossRef][Medline]
303. Argiles JM, Lopez-Soriano J, Busquets S, Lopez-Soriano FJ 1997 Journey from cachexia to obesity by TNF. FASEB J 11:743–751[Abstract]
304. Stephens JM, Pekala PH 1991 Transcriptional repression of the GLUT4 and C/EBP genes in 3T3–L1 adipocytes by tumor necrosis factor-. J Biol Chem 266:21839–21845[Abstract/Free Full Text]
305. Hotamisligil GS, Murray DL, Choy LN, Spiegelman BM 1994 Tumor necrosis factor inhibits signaling from the insulin receptor. Proc Natl Acad Sci USA 91:4854–4858[Abstract/Free Full Text]
306. Hotamisligil GS, Peraldi P, Budavari A, Ellis R, White MF, Spiegelman BM 1996 IRS1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF- and obesity-induced insulin resistance. Science 271:665–668[Abstract]
307. Valverde AM, Teruel T, Navarro P, Benito M, Lorenzo M 1998 Tumor necrosis factor- causes insulin receptor substrate-2-mediated insulin resistance and inhibits insulin-induced adipogenesis in fetal brown adipocytes. Endocrinology 139:1229–1238[Abstract/Free Full Text]
308. Uysal KT, Wiesbrock SM, Marino MW, Hotamisligil GS 1997 Protection from obesity-induced insulin resistance in mice lacking TNF- function. Nature 389:610–614[CrossRef][Medline]
309. Schreyer SA, Chua SC, LeBoeuf RC 1998 Obesity and diabetes in TNF- receptor-deficient mice. J Clin Invest 102:402–411[Medline]
310. Morin CL, Gayles EC, Podolin DA, Wei Y, Xu M, Pagliassotti MJ 1998 Adipose tissue-derived tumor necrosis factor activity correlates with fat cell size but not insulin action in aging rats. Endocrinology 139:4998–5005[Abstract/Free Full Text]
311. Zhang B, Berger J, Hu E, Szalkowski D, White-Carrington S, Spiegelman BM, Moller DE 1996 Negative regulation of peroxisome proliferator-activated receptor- gene expression contributes to the antiadipogenic effects of tumor necrosis factor-. Mol Endocrinol 10:1457–1466[Abstract/Free Full Text]
312. Rosenbaum SE, Greenberg AS 1998 The short- and long-term effects of tumor necrosis factor- and BRL 49653 on peroxisome proliferator-activated receptor (PPAR)2 gene expression and other adipocyte genes. Mol Endocrinol 12:1150–1160[Abstract/Free Full Text]
313. Souza SC, Yamamoto MT, Franciosa MD, Lien P, Greenberg AS 1998 BRL 49653 blocks the lipolytic actions of tumor necrosis factor-: a potential new insulin-sensitizing mechanism for thiazolidinediones. Diabetes 47:691–695[Abstract]
314. Murase K, Odaka H, Suzuki M, Tayuki N, Ikeda H 1998 Pioglitazone time-dependently reduces tumour necrosis factor- level in muscle and improves metabolic abnormalities in Wistar fatty rats. Diabetologia 41:257–264[CrossRef][Medline]
315. Peraldi P, Xu M, Spiegelman BM 1997 Thiazolidinediones block tumor necrosis factor -induced inhibition of insulin signaling. J Clin Invest 100:1863–1869[Medline]
316. Zhang YY, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM 1994 Positional cloning of the mouse obese gene and its human homologue. Nature 372:425–432[CrossRef][Medline]
317. Hwang CS, Loftus TM, Mandrup S, Lane MD 1997 Adipocyte differentiation and leptin expression. Annu Rev Cell Dev Biol 13:231–259[CrossRef][Medline]
318. Friedman JM, Halaas JL 1998 Leptin and the regulation of body weight in mammals. Nature 395:763–770[CrossRef][Medline]
319. Frederich RC, Hamann A, Anderson S, Lollmann B, Lowell BB, Flier JS 1995 Leptin levels reflect body lipid content in mice: evidence for diet-induced resistance to leptin action. Nat Med 1:1311–1314[CrossRef][Medline]
320. Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR, Ohannesian JP, Marco CC, McKee LJ, Bauer TL, Caro JF 1996 Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med 334:292–295[Abstract/Free Full Text]
321. Wang JL, Liu R, Hawkins M, Barzilai N, Rossetti L 1998 A nutrient-sensing pathway regulates leptin gene expression in muscle and fat. Nature 393:684–688[CrossRef][Medline]
322. Woods SC, Seeley RJ, Porte Jr D, Schwartz MW 1998 Signals that regulate food intake and energy homeostasis. Science 280:1378–1383[Abstract/Free Full Text]
323. De Vos P, Lefebvre AM, Miller SG, Guerre-Millo M, Wong K, Saladin R, Hamann LG, Staels B, Briggs MR, Auwerx J 1996 Thiazolidinediones repress ob gene expression in rodents via activation of peroxisome proliferator-activated receptor . J Clin Invest 98:1004–1009[Medline]
324. Kallen CB, Lazar MA 1996 Antidiabetic thiazolidinediones inhibit leptin (ob) gene expression in 3T3–L1 adipocytes. Proc Natl Acad Sci USA 93:5793–5796[Abstract/Free Full Text]
325. Zhang B, Graziano MP, Doebber TW, Leibowitz MD, White-Carrington S, Szalkowiski DM, Hey PJ, Wu M, Cullinan CA, Bailey P, Lollmann B, Frederich R, Flier JS, Strader CD, Smith RG 1996 Down-regulation of the expression of the Obese gene by an antidiabetic thiazolidinelione in Zucker diabetic fatty rats and db/db mice. J Biol Chem 271:9455–9459[Abstract/Free Full Text]
326. Hollenberg AN, Susulic VS, Madura JP, Zhang B, Moller DE, Tontonoz P, Sarraf P, Spiegelman BM, Lowell BB 1997 Functional antagonism between CCAAT/enhancer binding protein and peroxisome proliferator-activated receptor on the leptin promoter. J Biol Chem 272:5283–5290[Abstract/Free Full Text]
327. Ross SR, Graves RA, Spiegelman BM 1993 Targeted expression of a toxin gene to adipose tissue: transgenic mice resistant to obesity. Genes Dev 7:1318–1324[Abstract/Free Full Text]
328. Burant CF, Sreenan S, Hirano KI, Tai TAC, Lohmiller J, Lukens J, Davidson NO, Ross S, Graves RA 1997 Troglitazone action is independent of adipose tissue. J Clin Invest 100:2900–2908[Medline]
329. Shimabukuro M, Zhou YT, Levi M, Unger RH 1998 Fatty acid-induced beta cell apoptosis: a link between obesity and diabetes. Proc Natl Acad Sci USA 95:2498–2502[Abstract/Free Full Text]
330. Shimabukuro M, Zhou YT, Lee Y, Unger RH 1998 Troglitazone lowers islet fat and restores ß cell function of Zucker diabetic fatty rats. J Biol Chem 273:3547–3550[Abstract/Free Full Text]
331. Park KS, Ciaraldi TP, Lindgren K, Abrams-Carter L, Mudaliar S, Nikoulina SE, Tufari SR, Veerkamp JH, Vidal-Puig A, Henry RR 1998 Troglitazone effects on gene expression in human skeletal muscle of type II diabetes involve up-regulation of peroxisome proliferator-activated receptor-. J Clin Endocrinol Metab 83:2830–2835[Abstract/Free Full Text]
332. Zierath JR, Ryder JW, Doebber T, Woods J, Wu M, Ventre J, Li Z, McCrary C, Berger J, Zhang B, Moller DE 1998 Role of skeletal muscle in thiazolidinedione insulin sensitizer (PPAR agonist) action. Endocrinology 139:5034–5041[Abstract/Free Full Text]
333. Yokomizo T, Izumi T, Chang K, Takuwa Y, Shimizu T 1997 A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis. Nature 387:620–624[CrossRef][Medline]
334. von Schacky C, Kiefl R, Marcus AJ, Broekman MJ, Kaminski WE 1993 Dietary n-3 fatty acids accelerate catabolism of leukotriene B4 in human granulocytes. Biochim Biophys Acta 1166:20–24[Medline]
335. Couve AO, Koenig C, Santos MJ 1992 Induction of peroxisomal enzymes and a 64-kDa peptide in cultured mouse macrophages treated with clofibrate. Exp Cell Res 202:541–544[CrossRef][Medline]
336. Staels B, Koenig W, Habib A, Merval R, Lebret M, Torra IP, Delerive P, Fadel A, Chinetti G, Fruchart JC, Najib J, Maclouf J, Tedgui A 1998 Activation of human aortic smooth-muscle cells is inhibited by PPAR but not by PPAR activators. Nature 393:790–793[CrossRef][Medline]
337. Poynter ME, Daynes RA 1998 Peroxisome proliferator-activated receptor alpha activation modulates cellular redox status, represses nuclear factor-kappaB signaling, and reduces inflammatory cytokine production in aging. J Biol Chem 273:32833–32841[Abstract/Free Full Text]
338. Ricote M, Li AC, Willson TM, Kelly CJ, Glass CK 1998 The peroxisome proliferator-activated receptor is a negative regulator of macrophage activation. Nature 391:79–82[CrossRef][Medline]
339. Jiang C, Ting AT, Seed B 1998 PPAR-gamma agonists inhibit production of monocyte inflammatory cytokines. Nature 391:82–86[CrossRef][Medline]
340. Hattori Y, Hattori S, Kasai K 1999 Troglitazone upregulates nitric oxide synthesis in vascular smooth muscle cells. Hypertension 33:943–948[Abstract/Free Full Text]
341. Petrova T, Akama K, Van Eldik L 1999 Cyclopentone prostaglandins suppress activation of microglia: down-regulation of inducible nitric-oxide synthase by 15-deoxy-Delta12,14-prostaglandin J2. Proc Natl Acad Sci USA 96:4668–4673[Abstract/Free Full Text]
342. Lehmann JM, Lenhard JM, Oliver BB, Ringold GM, Kliewer SA 1997 Peroxisome proliferator-activated receptors and are activated by indomethacin and other non-steroidal anti-inflammatory drugs. J Biol Chem 272:3406–3410[Abstract/Free Full Text]
343. Ross R 1993 The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362:801–809[CrossRef][Medline]
344. Tontonoz P, Nagy L, Alvarez JG, Thomazy VA, Evans RM 1998 PPAR promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell 93:241–252[CrossRef][Medline]
345. Ricote M, Huang J, Fajas L, Li A, Welch J, Najib J, Witztum JL, Auwerx J, Palinski W, Glass CK 1998 Expression of the peroxisome proliferator-activated receptor (PPAR) in human atherosclerosis and regulation in macrophages by colony stimulating factors and oxidized low density lipoprotein. Proc Natl Acad Sci USA 95:7614–7619[Abstract/Free Full Text]
346. Marx N, Sukhova G, Murphy C, Libby P, Plutzky J 1998 Macrophages in human atheroma contain PPAR: differentiation-dependent peroxisomal proliferator-activated receptor (PPAR) expression and reduction of MMP-9 activity through PPAR activation in mononuclear phagocytes in vitro. Am J Pathol 153:17–23[Abstract/Free Full Text]
347. Ohlsson BG, Englund MC, Karlsson AL, Knutsen E, Erixon C, Skribeck H, Liu Y, Bondjers G, Wiklund O 1996 Oxidized low density lipoprotein inhibits lipopolysaccharide-induced binding of nuclear factor-B to DNA and the subsequent expression of tumor necrosis factor- and interleukin-1ß in macrophages. J Clin Invest 98:78–89[Medline]
348. Nagy L, Tontonoz P, Alvarez JG, Chen H, Evans RM 1998 Oxidized LDL regulates macrophage gene expression through ligand activation of PPAR. Cell 93:229–240[CrossRef][Medline]
349. Marx N, Schönbeck U, Lazar MA, Libby P, Plutzky J 1998 Peroxisome proliferator-activated receptor gamma activators inhibit gene expression and migration in human vascular smooth muscle cells. Circ Res 83:1097–1103[Abstract/Free Full Text]
350. Inoue I, Shino K, Noji S, Awata T, Katayama S 1998 Expression of peroxisome proliferator-activated receptor (PPAR) in primary cultures of human vascular endothelial cells. Biochem Biophys Res Commun 246:370–374[CrossRef][Medline]
351. Bishop-Bailey D, Hla T 1999 Endothelial cell apoptosis induced by the peroxisome proliferator-activated receptor (PPAR) ligand 15-deoxy-[Delta]12,14-prostaglandin J2. J Biol Chem 274:17042–17048[Abstract/Free Full Text]
352. Kato K, Satoh H, Endo Y, Yamada D, Midorikawa S, Sato W, Mizuno K, Fujita T, Tsukamoto K, Watanabe T 1999 Thiazolidinediones down-regulate plasminogen activator inhibitor type 1 expression in human vascular endothelial cells: a possible role for PPAR in endothelial function. Biochem Biophys Res Commun 258:431–435[CrossRef][Medline]
353. Marx N, Bourcier T, Sukhova GK, Libby P, Plutzky J 1999 PPARgamma activation in human endothelial cells increases plasminogen activator inhibitor type-1 expression: PPAR as a potential mediator in vascular disease. Arterioscler Thromb Vasc Biol 19:546–551[Abstract/Free Full Text]
354. Chinetti G, Griglio S, Antonucci M, Tora I, Delerive P, Majd Z, Fruchart JC, Chapman J, Najib J, Staels B 1998 Activation of proliferator-activated receptors and induces apoptosis of human monocyte-derived macrophages. J Biol Chem 273:25573–25580[Abstract/Free Full Text]
355. Rivier M, Safonova I, Lebrun P, Griffiths CEM, Ailhaud G, Michel S 1998 Differential expression of peroxisome proliferator-activated receptor subtypes during the differentiation of human keratinocytes. J Invest Dermatol 111:1116–1121[CrossRef][Medline]
356. Matsuura H, Adachi H, Smart RC, Xu X, Arata J, Jetten AM 1999 Correlation between expression of peroxisome proliferator-activated receptor ß and squamous differentiation in epidermal and tracheobronchial epithelial cells. Mol Cell Endocrinol 147:85–92[CrossRef][Medline]
357. Saitou M, Sugai S, Tanaka T, Shimouchi K, Fuchs E, Narumiya S, Kakizuka A 1995 Inhibition of skin development by targeted expression of a dominant-negative retinoic acid receptor. Nature 374:159–162[CrossRef][Medline]
358. Imakado S, Bickenbach JR, Bundman DS, Rothnagel JA, Attar PS, Wang XJ, Walczak VR, Wisniewski S, Pote J, Gordon JS, Heyman RA, Evans RM, Roop DR 1995 Targeting expression of a dominant-negative retinoic acid receptor mutant in the epidermis of transgenic mice results in loss of barrier function. Genes Dev 9:317–329[Abstract/Free Full Text]
359. Hanley K, Jiang Y, Crumrine D, Bass NM, Appel R, Elias PM, Williams ML, Feingold KR 1997 Activators of the nuclear hormone receptors PPAR and FXR accelerate the development of the fetal epidermal permeability barrier. J Clin Invest 100:705–712[Medline]
360. Hanley K, Jiang Y, He SS, Friedman M, Elias PM, Bikle DD, Williams ML, Feingold KR 1998 Keratinocyte differentiation is stimulated by activators of the nuclear hormone receptor PPAR. J Invest Dermatol 110:368–375[CrossRef][Medline]
361. Komuves LG, Hanley K, Jiang Y, Elias PM, Williams ML, Feingold KR 1998 Ligands and activators of nuclear hormone receptors regulate epidermal differentiation during fetal rat skin development. J Invest Dermatol 111:429–433[CrossRef][Medline]
362. Cattley RC, DeLuca J, Elcombe C, Fenner-Crisp P, Lake BG, Marsman DS, Pastoor TA, Popp JA, Robinson DE, Schwetz B, Tugwood J, Wahli W 1998 Do peroxisome proliferating compounds pose a hepatocarcinogenic hazard to humans? Regul Toxicol Pharmacol 27:47–60
363. Karam WG, Ghanayem BI 1997 Induction of replicative DNA synthesis and PPAR -dependent gene transcription by Wy-14 643 in primary rat hepatocyte and non-parenchymal cell co-cultures. Carcinogenesis 18:2077–2083[Abstract/Free Full Text]
364. Bayly AC, Roberts RA, Dive C 1994 Suppression of liver cell apoptosis in vitro by the non-genotoxic hepatocarcinogen and peroxisome proliferator nafenopin. J Cell Biol 125:197–203[Abstract/Free Full Text]
365. Roberts RA, James NH, Woodyatt NJ, Macdonald N, Tugwood JD 1998 Evidence for the suppression of apoptosis by the peroxisome proliferator activated receptor (PPAR ). Carcinogenesis 19:43–48[Abstract/Free Full Text]
366. Peters JM, Aoyama T, Cattley RC, Nobumitsu U, Hashimoto T, Gonzalez FJ 1998 Role of peroxisome proliferator-activated receptor in altered cell cycle regulation in mouse liver. Carcinogenesis 19:1989–1994[Abstract/Free Full Text]
367. Holden PR, Tugwood JD 1999 Peroxisome proliferator-activated receptor : role in rodent liver cancer and species differences. Mol Endocrinol 22:1–8
368. Gariot P, Barrat E, Mejean L, Pointel JP, Drouin P, Debry G 1983 Fenofibrate and human liver. Lack of proliferation of peroxisomes. Arch Toxicol 53:151–163[Medline]
369. Tugwood JD, Aldridge TC, Lambe KG, MacDonald N, Woodyatt NJ 1998 Peroxisome proliferator-activated receptor- and the pleiotropic responses to peroxisome proliferators. Arch Toxicol Suppl 20:377–386[Medline]
370. Woodyatt NJ, Lambe KG, Myers KA, Tugwood JD, Roberts RA 1999 The peroxisome proliferator (PP) response element upstream of the human acyl CoA oxidase gene is inactive among a sample human population: significance for species differences in response to PPs. Carcinogenesis 20:369–372[Abstract/Free Full Text]
371. Mukherjee R, Jow L, Noonan D, McDonnell DP 1994 Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators. J Steroid Biochem Mol Biol 51:157–166[CrossRef][Medline]
372. Shao D, Lazar MA 1997 Peroxisome proliferator activated receptor , CCAAT/enhancer-binding protein , and cell cycle status regulate the commitment to adipocyte differentiation. J Biol Chem 272:21473–21478[Abstract/Free Full Text]
373. Hansen JB, Petersen RK, Larsen BM, Bartkova J, Alsner J, Kristiansen K 1999 Activation of peroxisome proliferator-activated receptor bypasses the function of the retinoblastoma protein in adipocyte differentiation. J Biol Chem 274:2386–2393[Abstract/Free Full Text]
374. Altiok S, Xu M, Spiegelman BM 1997 PPAR induces cell cycle withdrawal: inhibition of E2F/DP DNA-binding activity via down-regulation of PP2A. Genes Dev 11:1987–1998[Abstract/Free Full Text]
375. Tontonoz P, Singer S, Forman BM, Sarraf P, Fletcher JA, Fletcher CDM, Brun RP, Mueller E, Altiok S, Oppenheim H, Evans RM, Spiegelman BM 1997 Terminal differentiation of human liposarcoma cells induced by ligands for peroxisome proliferator-activated receptor g and the retinoid X receptor. Proc Natl Acad Sci USA 94:237–241[Abstract/Free Full Text]
376. Morrison RF, Farmer SR 1999 Role of PPARgamma in regulating a cascade expression of cyclin-dependent kinase inhibitors, p18 (INK4c) and p21 (Waf1/Cip1), during adipogenesis. J Biol Chem 274:17088–17097[Abstract/Free Full Text]
377. Mueller E, Sarraf P, Tontonoz P, Evans RM, Martin KJ, Zhang M, Fletcher C, Singer S, Spiegelman BM 1998 Terminal differentiation of human breast cancer through PPAR. Mol Cell 1:465–470[CrossRef][Medline]
378. Elstner E, Müller C, Koshizuka K, Williamson EA, Park D, Asou H, Shintaku P, Said JW, Heber D, Koeffler HP 1998 Ligands for peroxisome proliferator-activated receptor and retinoic acid receptor inhibit growth and induce apoptosis of human breast cancer cells in vitro and in BNX mice. Proc Natl Acad Sci USA 95:8806–8811[Abstract/Free Full Text]
379. Kubota T, Koshizuka K, Williamson EA, Asou H, Said JW, Holden S, Miyoshi I, Koeffler HP 1998 Ligand for peroxisome proliferator-activated receptor gamma (troglitazone) has potent antitumor effect against human prostate cancer both in vitro and in vivo. Cancer Res 58:3344–3352[Abstract/Free Full Text]
380. Brockman JA, Gupta RA, Dubois RN 1998 Activation of PPARgamma leads to inhibition of anchorage-independent growth of human colorectal cancer cells. Gastroenterology 115:1049–1055[CrossRef][Medline]
381. Sarraf P, Mueller E, Jones D, King F, DeAngelo D, Partridge J, Holden S, Chen L, Singer S, Fletcher C, Spiegelman B 1998 Differentiation and reversal of malignant changes in colon cancer through PPAR. Nat Med 4:1046–1052[CrossRef][Medline]
382. Xin X, Yang S, Kowalski J, Gerritsen ME 1999 Peroxisome proliferator-activated receptor ligands are potent inhibitors of angiogenesis in vitro and in vivo. J Biol Chem 274:9116–9121[Abstract/Free Full Text]
383. Seed B 1998 PPAR and colorectal carcinoma: conflicts in a nuclear family. Nat Med 4:1004–1005[CrossRef][Medline]
384. Onate SA, Boonyaratanakornkit V, Spencer TE, Tsai SY, Tsai MJ, Edwards DP, O’Malley BW 1998 The steroid receptor coactivator-1 contains multiple receptor interacting and activation domains that cooperatively enhance the activation function 1 (AF1) and AF2 domains of steroid receptors. J Biol Chem 273:12101–12108[Abstract/Free Full Text]
385. Takeshita A, Yen PM, Misiti S, Cardona GR, Liu Y, Chin WW 1996 Molecular cloning and properties of a full-length putative thyroid hormone receptor coactivator. Endocrinology 137:3594–3597[Abstract]
386. Tagami T, Lutz WH, Kumar R, Jameson JL 1998 The interaction of the vitamin D receptor with nuclear receptor corepressors and coactivators. Biochem Biophys Res Commun 253:358–363[CrossRef][Medline]
387. Kraus WL, Kadonaga JT 1998 p300 And estrogen receptor cooperatively activate transcription via differential enhancement of initiation and reinitiation. Genes Dev 12:331–342[Abstract/Free Full Text]
388. Aarnisalo P, Palvimo JJ, Janne OA 1998 CREB-binding protein in androgen receptor-mediated signaling. Proc Natl Acad Sci USA 95:2122–2127[Abstract/Free Full Text]
389. Gelman L, Zhou G, Fajas L, RaspÈ, Fruchart JC, Auwerx J 1999 p300 Interacts with the N- and C-terminal part of PPAR2 in a ligand-independent and -dependent manner, respectively. J Biol Chem 274:7681–7688[Abstract/Free Full Text]
390. Castillo G, Brun RP, Rosenfield JK, Hauser S, Won Park C, Troy AE, Wright ME, Spiegelman BM 1999 An adipogenic cofactor bound by the differentiation domain of PPAR. EMBO J 18:3676–3687[CrossRef][Medline]
391. Lee CH, Chinpaisal C, Wei LN 1998 Cloning and characterization of mouse RIP140, a corepressor for nuclear orphan receptor TR2. Mol Cell Biol 18:6745–6755[Abstract/Free Full Text]
392. Yeh S, Chang C 1996 Cloning and characterization of a specific coactivator, ARA70, for the androgen receptor in human prostate cells. Proc Natl Acad Sci USA 93:5517–5521[Abstract/Free Full Text]
393. Heinlein CA, Ting HJ, Yeh S, Chang C 1999 Identification of ARA70 as a ligand-enhanced coactivator for the peroxisome proliferator-activated receptor . J Biol Chem 274:16147–16152[Abstract/Free Full Text]
394. Chen JD, Umesono K, Evans RM 1996 SMRT isoforms mediate repression and anti-repression of nuclear receptor heterodimers. Proc Natl Acad Sci USA 93:7567–7571[Abstract/Free Full Text]
395. Chen JD, Evans RM 1995 A transcriptional co-repressor that interacts with nuclear hormone receptors. Nature 377:454–457[CrossRef][Medline]
396. Shibata H, Nawaz Z, Tsai SY, O’Malley BW, Tsai MJ 1997 Gene silencing by chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI) is mediated by transcriptional corepressors, nuclear receptor-corepressor (N-CoR) and silencing mediator for retinoic acid receptor and thyroid hormone receptor (SMRT). Mol Endocrinol 11:714–724[Abstract/Free Full Text]
397. Kurokawa R, Soderstrom M, Horlein A, Halachmi S, Brown M, Rosenfeld MG, Glass CK 1995 Polarity-specific activities of retinoic acid receptors determined by a co-repressor. Nature 377:451–454[CrossRef][Medline]
398. Horlein AJ, Näär AM, Heinzel T, Torchia J, Gloss B, Kurokawa R, Ryan A, Kamel Y, Soderstrom M, Glass CK, Rosenfeld MG 1995 Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor. Nature 377:397–404[CrossRef][Medline]
399. Zamir I, Harding HP, Atkins GB, Horlein A, Glass CK, Rosenfeld MG, Lazar MA 1996 A nuclear hormone receptor corepressor mediates transcriptional silencing by receptors with distinct repression domains. Mol Cell Biol 16:5458–5465[Abstract]
400. Dowell P, Ishmael J, Avram D, Peterson V, Nevrivy D, Leid M 1999 Identification of nuclear corepressor as peroxisome proliferator-activated receptor interacting protein. J Biol Chem 274:15901–15907[Abstract/Free Full Text]
401. Osumi T, Wen JK, Hashimoto T 1991 Two cis-acting regulatory sequences in the peroxisome proliferator-responsive enhancer region of rat acyl-CoA oxidase gene. Biochem Biophys Res Commun 175:866–871[CrossRef][Medline]
402. Varanasi U, Chu R, Huang Q, Castellon R, Yeldandi AV, Reddy JK 1998 Additions and corrections to Identification of a peroxisome proliferator-responsive element upstream of the human peroxisomal fatty acyl coenzyme A oxidase gene. J Biol Chem 273:30842[Free Full Text]
403. Vu-Dac N, Schoonjans K, Laine B, Fruchart JC, Auwerx J, Staels B 1994 Negative regulation of the human apolipoprotein A-I promoter by fibrates can be attenuated by the interaction of the peroxisome proliferator-activated receptor with its response element. J Biol Chem 269:31012–31018[Abstract/Free Full Text]
404. Vu-Dac N, Schoonjans K, Kosykh V, Dallongeville J, Fruchart JC, Staels B, Auwerx J 1995 Fibrates increase human apolipoprotein A-II expression through activation of the peroxisome proliferator-activated receptor. J Clin Invest 96:741–750
405. Hertz R, Bishara-Shieban J, Bar-Tana J 1995 Mode of action of peroxisome proliferators as hypolipidemic drugs. J Biol Chem 270:13470–13475[Abstract/Free Full Text]
406. Bardot O, Aldridge TC, Latruffe N, Green S 1993 PPAR-RXR heterodimer activates a peroxisome proliferator response element upstream of the bifunctional enzyme gene. Biochem Biophys Res Commun 192:37–45[CrossRef][Medline]
407. Issemann I, Prince R, Tugwood J, Green S 1992 A role for fatty acid and liver fatty acid binding protein in peroxisome proliferation? Biochem Soc Trans 20:824–827[Medline]
408. Simonson GD, Iwanij V 1995 Genomic organization and promoter sequence of a gene encoding a rat liver-specific type-I transport protein. Gene 154:243–247[CrossRef][Medline]

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				C. D. Kane, K. A. Stevens, J. E. Fischer, M. Haghpassand, L. J. Royer, C. Aldinger, K. T. Landschulz, P. Zagouras, S. W. Bagley, W. Hada, et al. Molecular Characterization of Novel and Selective Peroxisome Proliferator-Activated Receptor {alpha} Agonists with Robust Hypolipidemic Activity in Vivo Mol. Pharmacol., February 1, 2009; 75(2): 296 - 306. [Abstract] [Full Text] [PDF]

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				M. T. Bility, M. K. Devlin-Durante, N. Blazanin, A. B. Glick, J. M. Ward, B. H. Kang, M. J. Kennett, F. J. Gonzalez, and J. M. Peters Ligand activation of peroxisome proliferator-activated receptor {beta}/{delta} (PPAR{beta}/{delta}) inhibits chemically induced skin tumorigenesis Carcinogenesis, December 1, 2008; 29(12): 2406 - 2414. [Abstract] [Full Text] [PDF]

				Y. H. Yu, E. C. Lin, S. C. Wu, W. T. K. Cheng, H. J. Mersmann, P. H. Wang, and S. T. Ding Docosahexaenoic acid regulates adipogenic genes in myoblasts via porcine peroxisome proliferator-activated receptor {gamma} J Anim Sci, December 1, 2008; 86(12): 3385 - 3392. [Abstract] [Full Text] [PDF]

				J. M. Mercader, J. J. Lozano, L. Sumoy, M. Dierssen, J. Visa, M. Gratacos, and X. Estivill Hypothalamus transcriptome profile suggests an anorexia-cachexia syndrome in the anx/anx mouse model Physiol Genomics, November 12, 2008; 35(3): 341 - 350. [Abstract] [Full Text] [PDF]

				M. W. Ruddock, A. Stein, E. Landaker, J. Park, R. C. Cooksey, D. McClain, and M.-E. Patti Saturated Fatty Acids Inhibit Hepatic Insulin Action by Modulating Insulin Receptor Expression and Post-receptor Signalling J. Biochem., November 1, 2008; 144(5): 599 - 607. [Abstract] [Full Text] [PDF]

				K. Vanschoonbeek, K. Wouters, P. E.J. van der Meijden, P. J. van Gorp, M. A.H. Feijge, M. Herfs, L. J. Schurgers, M. H. Hofker, M. P.M. de Maat, and J. W.M. Heemskerk Anticoagulant Effect of Dietary Fish Oil in Hyperlipidemia: A Study of Hepatic Gene Expression in APOE2 Knock-in Mice Arterioscler. Thromb. Vasc. Biol., November 1, 2008; 28(11): 2023 - 2029. [Abstract] [Full Text] [PDF]

				H. A. Hostetler, L. R. Syler, L. N. Hall, G. Zhu, F. Schroeder, and A. B. Kier A Novel High-Throughput Screening Assay for Putative Antidiabetic Agents through PPAR{alpha} Interactions J Biomol Screen, October 1, 2008; 13(9): 855 - 861. [Abstract] [PDF]

				F. Zapata-Gonzalez, F. Rueda, J. Petriz, P. Domingo, F. Villarroya, J. Diaz-Delfin, M. A. de Madariaga, and J. C. Domingo Human dendritic cell activities are modulated by the omega-3 fatty acid, docosahexaenoic acid, mainly through PPAR{gamma}:RXR heterodimers: comparison with other polyunsaturated fatty acids J. Leukoc. Biol., October 1, 2008; 84(4): 1172 - 1182. [Abstract] [Full Text] [PDF]

				T. D. Wiggin, M. Kretzler, S. Pennathur, K. A. Sullivan, F. C. Brosius, and E. L. Feldman Rosiglitazone Treatment Reduces Diabetic Neuropathy in Streptozotocin-Treated DBA/2J Mice Endocrinology, October 1, 2008; 149(10): 4928 - 4937. [Abstract] [Full Text] [PDF]

				N. Dobrosi, B. I. Toth, G. Nagy, A. Dozsa, T. Geczy, L. Nagy, C. C. Zouboulis, R. Paus, L. Kovacs, and T. Biro Endocannabinoids enhance lipid synthesis and apoptosis of human sebocytes via cannabinoid receptor-2-mediated signaling FASEB J, October 1, 2008; 22(10): 3685 - 3695. [Abstract] [Full Text] [PDF]

				G. Bryzgalova, L. Lundholm, N. Portwood, J.-A. Gustafsson, A. Khan, S. Efendic, and K. Dahlman-Wright Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice Am J Physiol Endocrinol Metab, October 1, 2008; 295(4): E904 - E912. [Abstract] [Full Text] [PDF]

				G. J. Ko, Y. S. Kang, S. Y. Han, M. H. Lee, H. K. Song, K. H. Han, H. K. Kim, J. Y. Han, and D. R. Cha Pioglitazone attenuates diabetic nephropathy through an anti-inflammatory mechanism in type 2 diabetic rats Nephrol. Dial. Transplant., September 1, 2008; 23(9): 2750 - 2760. [Abstract] [Full Text] [PDF]

				N. Petrovic, I. G. Shabalina, J. A. Timmons, B. Cannon, and J. Nedergaard Thermogenically competent nonadrenergic recruitment in brown preadipocytes by a PPAR{gamma} agonist Am J Physiol Endocrinol Metab, August 1, 2008; 295(2): E287 - E296. [Abstract] [Full Text] [PDF]

				T. Genovese, E. Esposito, E. Mazzon, R. D. Paola, R. Meli, P. Bramanti, D. Piomelli, A. Calignano, and S. Cuzzocrea Effects of Palmitoylethanolamide on Signaling Pathways Implicated in the Development of Spinal Cord Injury J. Pharmacol. Exp. Ther., July 1, 2008; 326(1): 12 - 23. [Abstract] [Full Text] [PDF]

				M. Bionaz, C. R. Baumrucker, E. Shirk, J. P. Vanden Heuvel, E. Block, and G. A. Varga Short Communication: Characterization of Madin-Darby Bovine Kidney Cell Line for Peroxisome Proliferator-Activated Receptors: Temporal Response and Sensitivity to Fatty Acids J Dairy Sci, July 1, 2008; 91(7): 2808 - 2813. [Abstract] [Full Text] [PDF]

				M. Schwab, V. Reynders, S. Loitsch, Y. M. Shastri, D. Steinhilber, O. Schroder, and J. Stein PPAR{gamma} is involved in mesalazine-mediated induction of apoptosis and inhibition of cell growth in colon cancer cells Carcinogenesis, July 1, 2008; 29(7): 1407 - 1414. [Abstract] [Full Text] [PDF]

				M. Iemitsu, N. Shimojo, S. Maeda, Y. Irukayama-Tomobe, S. Sakai, T. Ohkubo, Y. Tanaka, and T. Miyauchi The benefit of medium-chain triglyceride therapy on the cardiac function of SHRs is associated with a reversal of metabolic and signaling alterations Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H136 - H144. [Abstract] [Full Text] [PDF]

				J. J. Lehman, S. Boudina, N. H. Banke, N. Sambandam, X. Han, D. M. Young, T. C. Leone, R. W. Gross, E. D. Lewandowski, E. D. Abel, et al. The transcriptional coactivator PGC-1{alpha} is essential for maximal and efficient cardiac mitochondrial fatty acid oxidation and lipid homeostasis Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H185 - H196. [Abstract] [Full Text] [PDF]

				D Bonofiglio, H Qi, S Gabriele, S Catalano, S Aquila, M Belmonte, and S Ando Peroxisome proliferator-activated receptor {gamma} inhibits follicular and anaplastic thyroid carcinoma cells growth by upregulating p21Cip1/WAF1 gene in a Sp1-dependent manner Endocr. Relat. Cancer, June 1, 2008; 15(2): 545 - 557. [Abstract] [Full Text] [PDF]

				M. Collino, N. S.A. Patel, and C. Thiemermann Review: PPARs as new therapeutic targets for the treatment of cerebral ischemia/reperfusion injury Therapeutic Advances in Cardiovascular Disease, June 1, 2008; 2(3): 179 - 197. [Abstract] [PDF]

				M. B. Rosen, B. D. Abbott, D. C. Wolf, J. C. Corton, C. R. Wood, J. E. Schmid, K. P. Das, R. D. Zehr, E. T. Blair, and C. Lau Gene Profiling in the Livers of Wild-type and PPAR{alpha}-Null Mice Exposed to Perfluorooctanoic Acid Toxicol Pathol, June 1, 2008; 36(4): 592 - 607. [Abstract] [Full Text] [PDF]

				A. Montoudis, E. Seidman, F. Boudreau, J.-F. Beaulieu, D. Menard, M. Elchebly, G. Mailhot, A.-T. Sane, M. Lambert, E. Delvin, et al. Intestinal fatty acid binding protein regulates mitochondrion {beta}-oxidation and cholesterol uptake J. Lipid Res., May 1, 2008; 49(5): 961 - 972. [Abstract] [Full Text] [PDF]