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Endocrine Reviews 20 (3): 253-278
Copyright © 1999 by The Endocrine Society

Tamoxifen, Raloxifene, and the Prevention of Breast Cancer1

V. Craig Jordan and Monica Morrow

Departments of Molecular Pharmacology, Biological Chemistry (V.C.J.), and Surgery (M.M.), Robert H. Lurie Comprehensive Cancer Center, Northwestern University Medical School, Chicago, Illinois 60611


    Abstract
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 

I. Introduction
II. Lacassagne’s Prevention Principle: A Target and an Estrogen Antagonist
III. Tamoxifen as an Antitumor Agent
A. ER status and the duration of tamoxifen
B. Contralateral breast cancer
C. Endometrial cancer
D. Conclusions
IV. Selective Estrogen Receptor Modulation
A. Antiestrogen activity at the ER
B. Coactivators for ER
C. Alternate response elements on DNA
D. An alternate ER-ERß
V. Biological Basis for Tamoxifen as a Breast Cancer Preventive
A. Animal models
B. Bones
C. Lipids
D. Uterus
VI. Risk Factors for Breast Cancer
A. Interactions among risk factors
B. Identification of candidates for chemoprevention
VII. Prevention of Breast Cancer with Tamoxifen
A. Royal Marsden Pilot Study
B. NSABP/NCI Study
C. Italian study
D. Conclusions
VIII. Biological Basis for Raloxifene as a Breast Cancer Preventive
A. Antitumor actions
B. Bones
C. Lipids
D. Uterus
IX. Study of Tamoxifen And Raloxifene (STAR)
X. The Future of Prevention


    I. Introduction
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 
IN 1896, George Beatson demonstrated that the removal of the ovaries from premenopausal women with metastatic breast cancer could, in some cases, cause regression of the disease and improve the prognosis of the patient (1). However, by 1900 Stanley Boyd had established that only one in three patients would obtain improvement for about 1 yr (2). Despite this disappointment, a link was established between an ovarian factor and the growth of some breast cancers. This observation was to become the foundation of modern clinical practice and the rationale for the use of antiestrogens to treat breast cancer (3, 4). At the turn of the century, studies were being conducted in the laboratory to complement the clinical effort. Inbred strains of mice were being established for medical research, and it was found that certain strains of mice developed a high incidence of mammary tumors. Lathrop and Loeb (5) reported that an early ovariectomy could prevent the spontaneous development of tumors but it was not until Allen and Doisey (6) identified "estrus stimulating principle" that ovarian hormones could be linked to the development of breast cancer. By 1936, Professor Antoine Lacassagne, again working with high-incidence strains of mice, suggested that if breast cancer was caused by a special hereditary sensitivity to estrogen, then the disease could be prevented by developing a therapeutic antagonist to estrogen action in the breast (7). However, there were no therapeutic antagonists of estrogen at that time, nor was there a target to design drug molecules. Nevertheless, exciting developments in the discovery of nonsteroidal estrogens would establish the structural basis of carrier molecules, which resulted in the design of the two drugs, tamoxifen and raloxifene (Fig. 1Go), both originally described as antiestrogens and used today in a clinical trial to prevent breast cancer in high-risk women (see Section IX).



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Figure 1. The discovery of MER-25, and the knowledge that a strategically placed alkylaminoethoxy side chain confers antiestrogenic properties, was important to develop the antiestrogens tamoxifen and raloxifene from the known nonsteroidal estrogens, triphenylethylene and diethylstilbestrol. Tamoxifen is used for the endocrine treatment of all stages of breast cancer and is available for the reduction of breast cancer incidence in high-risk women. Raloxifene is used for the prevention of osteoporosis in postmenopausal women, but because a preliminary evaluation shows a reduction in the risk of breast cancer, raloxifene is to be evaluated for the prevention of breast cancer in high-risk postmenopausal women. The clinical trial STAR started to recruit the 22,000 volunteers in 1999.

 
Estrogen action in the 1930s was assayed using ovariectomized mice as originally described by Allen and Doisy (6). Using this technique, parallel research ventures resulted in the discovery of the triphenylethylene-based estrogens (8, 9, 10) and the stilbene-based estrogens (Fig. 1Go). The triphenylethylenes are long acting and are stored in body fat (11, 12, 13, 14), whereas the hydroxystilbene derivatives are short acting (9, 15, 16), primarily because of rapid-phase II metabolism after absorption. However, Dodds and associates (17, 18) described an extremely potent compound, diethylstilbestrol (Fig. 1Go), that was widely used in gynecology and also subsequently used, at high doses, as a treatment for advanced breast cancer in postmenopausal women (19, 20).

In the 1950s and 1960s it became clear that adrenalectomy, with glucocorticoid support, could also improve the prognosis of some postmenopausal women with advanced breast cancer (21). In fact, about one third of the women responded, i.e., about the same proportion as premenopausal women after oophorectomy. The reason for the apparently arbitrary responses, however, would not become clear until the discovery of the estrogen receptor (ER) by Jensen and Jacobson (22) and the subsequent application of this knowledge to predict the hormone responsiveness of a patient’s tumor to endocrine ablation (23). This was an extremely important finding because it prevented those patients who had an ER-negative tumor from having additional major surgery with little hope of a response. Only patients whose tumors had high levels of ER were likely to respond to endocrine ablative surgery (24). It is important, however, to stress that in the early 1970s, there was no significant clinical experience available with the class of drugs called antiestrogens, and no large clinical studies had linked the efficacy of antiestrogens with the presence or absence of the ER. Several antiestrogens had been tested in small clinical studies, but tamoxifen, the first clinically useful antiestrogen for the treatment of advanced breast cancer in postmenopausal women, was not approved by the Food and Drug Administration in the United States until 1977 (25).

Although antiestrogens are important therapeutic agents today, 40 yr ago there was very little interest in treating breast cancer with new hormonal drugs, and most of the research in endocrinology was focused on an understanding of reproduction. The discovery of the nonsteroidal antiestrogens was serendipitous and resulted from an interest in contraception in the 1950s. The first nonsteroidal antiestrogen to be reported in the literature, MER25 (Fig. 1Go), was described by Lerner and co-workers in 1958 (26) as an agent that had no other hormonal or antihormonal properties in any species tested. In fact, it was a blocking drug for estrogen action with almost no estrogenic properties. The drug failed in clinical trial because large doses were required (MER25 has low potency), which caused serious central nervous system side effects (27). On one hand this was disappointing but, it must be stressed, that a pure antiestrogen such as MER25 would ultimately have been catastrophic as an agent to prevent breast cancer. Drug discovery switched to the triphenylethylene-based compounds that resulted first in clomiphene and then tamoxifen (25). Subsequently, drug discovery concentrated on compounds with a high affinity for ER (25). Only a research focus on cancer in the 1970s facilitated tamoxifen’s development as a breast cancer therapy for all stages of the disease (25).

The critical property of the so called "antiestrogens," which permitted their subsequent development as long-term preventives for breast cancer, was that they are antiestrogens at some sites, like the breast, but had estrogen-like properties at other sites to maintain bone density and lower circulating cholesterol (28, 29, 30, 31, 32, 33, 34, 35). The unusual target site-specific action as an estrogen or as an antiestrogen was true for both tamoxifen and raloxifene (29, 30). Lacassagne’s prediction (7) of developing an antagonist to estrogen action to prevent breast cancer in healthy women would not have occurred if the available drugs had increased the risk of osteoporosis and coronary heart disease.

The goal of this review is to provide an up-to-date analysis of the current status of efforts to prevent breast cancer in women by the strategic use of antiestrogens. One aim of our review is to identify the principles, established in the laboratory, that have, through the clinical trial process, proven to be valid in patients with breast cancer or women at risk for breast cancer. The review will also provide the scientific basis for the ongoing trial called Study of Tamoxifen And Raloxifene (STAR). This trial is examining the worth of raloxifene, a drug approved for the prevention of osteoporosis, to prevent breast cancer in postmenopausal women with elevated risk factors. The biological basis for consideration of the antiestrogen raloxifene to be used as a breast cancer preventive is described in Section VIII. In the interests of space it is not possible to review the antiestrogen literature exhaustively, but we intend to provide sufficient background to link laboratory research with clinical results.


    II. Lacassagne’s Prevention Principle: A Target and an Estrogen Antagonist
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 
In 1962, Jensen and Jacobson (22) demonstrated that [3H]estradiol bound to and was retained by estrogen target tissue, e.g., uterus, vagina, and pituitary gland, in the immature female rat. By contrast, tissues that did not respond to estrogen did not retain [3H]estradiol. Jensen proposed that an ER must be present in estrogen target tissues to capture circulating steroids and initiate the cascade of biochemical events associated with estrogen action in that particular tissue. Gorski and co-workers (36, 37) first identified the ER as an extractable protein from rat uterus. Subsequently, the groups of Gorski et al. (38) and Jensen et al. (39) independently proposed subcellular models of estrogen action in target tissues. However, Jensen and associates (23) took the process one step further by proposing the clinical ER assay to predict hormone-responsive breast cancer. Thus, a mechanistic link between estrogen action and the growth of breast cancer was established.

The MCF-7 breast cancer cell line is ER positive (40, 41), and the cells have found ubiquitous applications in cancer research laboratories throughout the world (42). Most importantly, access to these cells has resulted in a fundamental change in the understanding of hormone action that has resulted in the discovery of the steroid receptor superfamily of receptors (43, 44). Jensen and co-workers (45, 46) first developed monoclonal antibodies to ER derived from MCF-7 cells. The antibodies were used to establish that the ER was a nuclear protein (47), and the technology of immunocytochemistry is now standard for the determination of receptor status in breast biopsies (48, 49). However, the application of monoclonal antibodies as probes to clone and sequence the ER gene (50, 51, 52) is of fundamental significance for the understanding of the ER as a nuclear transcription factor.

The ER is a nuclear protein (47, 53, 54), which, for convenience, is subdivided into six functional domains (Fig. 2Go) (55, 56). The E regions make up the steroid-binding domain that undergoes a conformation change to lock estradiol into its hydrophobic pocket (see Section IV.A). Changes in conformation of the ER permits the binding of coactivators to the activating function 1 and 2 regions (AF-1 and 2) (57, 58, 59) and facilitates the interaction of the ER with DNA through the DNA-binding domain (C region) (60, 61). The transcription unit is selectively located as a homodimer in the promoter region of estrogen-responsive genes to initiate the events associated with estrogen-stimulated cell replication. Unfortunately, it is not possible to provide much more than the basic concepts in hormone action because our article is focused on progress in breast cancer chemoprevention. The topic of gene regulation is a rapidly evolving story, so we strongly recommend that interested readers consult recently published reviews that provide further information (62, 63, 64).



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Figure 2. A subcellular model of estradiol (E2) action in a target tissue. Estradiol diffuses into all cells but binds to the ER specifically located in estrogen target tissues (e.g., uterus, vagina, some breast cancers, etc.). The steroid receptor complex undergoes a conformational change and dimerizes before binding to an estrogen response element (ERE) in the promotor region of an estrogen-responsive gene. A transcription unit is formed by interaction with coactivator (CoA) molecules to initiate RNA synthesis and ultimately the estrogen-stimulated cellular response. Corepressor (CoR) modules are believed to prevent transcription by exclusively interacting with antiestrogen ER complexes. The ER is divided into six regions (A–F). The DNA-binding domain (C) is essential for the interaction of the ER with the ERE. The ligand-binding domain (E) is the site of E2 binding and the site of competitive binding by antiestrogens. The AA351 is identified in the E region because it is the known site of an interaction with the alkylaminoethoxy side chain of raloxifene. The activating function (AF-1 and -2) regions are the areas of the ER that interact with coactivators to form the transcription unit at an estrogen-responsive gene.

 
The second facet of Lacassagne’s hypothesis is the requirement for an antiestrogen to block estrogen action. Tamoxifen blocks the binding of [3H]estradiol to the ER derived from rat uterus (65, 66, 67, 68) or human tumor (69, 70). However, initial clinical studies with tamoxifen were conducted exclusively on unselected populations of postmenopausal women with advanced breast cancer (3, 4), and not until 1977 was it noted that tamoxifen was more likely to be effective in ER-positive breast cancer (71). Tamoxifen is currently used as a palliative therapy in the treatment of pre- and postmenopausal patients with ER-positive advanced (Stage IV) breast cancer. By contrast, the application of the concept of adjuvant therapy has revolutionized the treatment of breast cancer. Systemic adjuvant therapy is used after breast surgery to destroy undetected micrometastases around a woman’s body.

Adjuvant studies with tamoxifen have proved to be successful in increasing survival (72, 73, 74) but, perhaps most importantly, the interaction between laboratory and clinical research endeavors has ultimately elucidated both the principal mechanism of action of tamoxifen as an antitumor agent in women and identified those women most likely to benefit from adjuvant tamoxifen treatment. During the past 12 yr there has been some confusion in the literature about whether tamoxifen was active in ER-positive breast cancer exclusively or whether ER-negative breast cancer could respond (75, 76, 77). Additionally, there was controversy as to whether tamoxifen was significantly active as an adjuvant in premenopausal women (78). The reader is referred to recent reviews on the clinical investigation and development of tamoxifen (79, 80, 81, 82, 83) for further information, but we will summarize the latest findings of the world-wide randomized clinical trials (84). It is important to appreciate that the general principles derived from the use of tamoxifen as a therapy for breast cancer can be used as a basis for consideration of tamoxifen as a estrogen antagonist for the prevention of breast cancer.


    III. Tamoxifen as an Antitumor Agent
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 
The 1998 Oxford Overview Analysis (84) involved any randomized trial that began before 1990. The analysis included 55 trials of adjuvant tamoxifen vs. no tamoxifen before recurrence. The study population comprised 37,000 women with node-positive and node-negative breast cancer, thus comprising 87% of world evidence of known randomized clinical trials. Of these women, fewer than 8,000 had a very low or zero level of ER and 18,000 were classified as ER positive. The ER status of the remaining nearly 12,000 women was unknown, but based on the normal distribution of ER in random populations, the authors estimated that two-thirds would be ER positive.

This clinical trial data base (84) can now be used to answer the questions raised over the past two decades by laboratory results and hypotheses. In the 1970s three laboratory observations emerged that merited evaluation in clinical trial: 1) tamoxifen blocks estrogen binding to the ER, making patients with ER-positive disease more likely to respond than those with ER-negative disease (85), 2) tamoxifen prevents mammary cancer in rats (86, 87) so the drug could reduce the incidence of primary breast cancer, and 3) long-term treatment was better than short-term treatment to prevent rat mammary carcinogenesis; therefore, longer adjuvant therapy with tamoxifen should be superior to short-term adjuvant therapy (88, 89, 90), i.e., 5 yr of tamoxifen should be superior to 1 yr of tamoxifen. By the late 1980s, tamoxifen had been shown in the laboratory to block estrogen-stimulated breast tumor growth but to encourage the growth of human endometrial cancer implanted in the same athymic mouse (91, 92). The clinical question therefore became "are patients, who are receiving long-term adjuvant tamoxifen therapy, at risk for an increased incidence of endometrial cancer?" (92).

The process of evaluating the impact of translational research is important to establish what works and achieves clinical progress and what does not. A clinical trial should not begin without a strong hypothesis and the incorporation of relevant scientific results. For convenience, the discussion in this section will be subdivided, but the end points of duration of tamoxifen usage, menopausal status, and ER status interact, making the size of a pharmacological effect subject to change.

A. ER status and the duration of tamoxifen
The ER status of the patient is highly predictive of a treatment response to long-term tamoxifen therapy. The treatment effect, based on receptor status, is summarized in Table 1Go. The recurrence reductions produced by tamoxifen in ER-positive patients are all highly significant (2P < 0.00001), and the trend between the different durations of tamoxifen is also highly significant ({chi}2 = 45.5, 2P < 0.00001). By contrast, the therapeutic effect of tamoxifen on ER-negative patients is minimal. Additionally, the questions could be asked, "Does more ER give a better response to tamoxifen?" and "Does an additional progesterone receptor (PgR) assay help to improve the results with tamoxifen?" In the trials of about 5 yr of tamoxifen treatment, the proportional reductions of recurrence were 43 ± 5% and 60 ± 6% for patients with below or above 100 fmol/mg cytosol ER protein. This translated to a reduction in mortality of 23 ± 6% and 36 ± 7%, respectively. Clearly, one can conclude the ER is a powerful predictor of tamoxifen response, a conclusion consistent with tamoxifen’s proven mechanism of action as an estrogen antagonist in breast cancer (82). Although PgR-positive status might be thought to be of benefit, these data show that there was little additional value if the tumor was already ER positive. A comparison of interactions is shown in Table 2Go. A comparison of the 2,000 women who had ER-positive and PgR-negative tumors and the 7,000 women who had ER-positive and PgR-positive tumors shows there was no apparent difference in the effect of tamoxifen on either the recurrence rates or mortality rates. Additionally, the numbers were too few (602 women) in the Overview Analysis (84) to allow a meaningful prediction of the benefits of tamoxifen in patients who had an ER-negative but PgR-positive tumor.


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Table 1. A comparison of the proportional risk reduction of adjuvant tamoxifen therapy based on ER status

 

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Table 2. A comparison of the proportional risk reduction of adjuvant tamoxifen therapy based on PgR status in populations of ER-positive patients

 
The Overview Analysis also provides unequivocal proof of the laboratory principle (88, 89, 90) that longer adjuvant tamoxifen therapy was predicted to provide more benefit. The duration of therapy is extremely important for the ER-positive premenopausal woman with large amounts of circulating estrogen that can rapidly reverse the effect of short-term tamoxifen treatment. The effect of the duration of tamoxifen treatment on the reduction of recurrence rates and the reduction of death rates is shown in Fig. 3Go. The duration of tamoxifen therapy is critical for the premenopausal patient: the effect of 1 yr of treatment is virtually nonexistent compared with the benefit of 5 yr of treatment. It is also important to point out that the reduction of death rates in women under 50 yr of age and over 60 yr of age treated with 5 yr of tamoxifen is identical, at around 33% (Table 3Go). By contrast, the effect of tamoxifen duration on women over the age of 60 is less dramatic because 1 yr of tamoxifen is much more effective in postmenopausal women. These data are illustrated in Table 3Go, which shows a 2- to 3-fold increase in the effectiveness of tamoxifen when the duration is increased from 1 to 5 yr, whereas there is a 20-fold increase in tamoxifen’s effectiveness for premenopausal women with an increased duration of 1–5 yr (Fig. 3Go).



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Figure 3. The relationship between the duration of adjuvant tamoxifen therapy in ER-positive premenopausal patients and the reduction in recurrence and death rate. A longer duration of treatment has a dramatic effective on patient survival. [Derived from Ref. 84.]

 

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Table 3. Proportional risk reductions in 60- to 69-yr-old breast cancer patients when the known ER-poor patients are excluded

 
B. Contralateral breast cancer
Tamoxifen consistently reduces the risk of contralateral breast cancer (i.e., a second primary breast cancer in the other breast) independent of age (84). Women have a proportional risk reduction that is 27 ± 11% or 31 ± 7% if they are below or above the age of 50, respectively. The principle "longer is better" is also true for the reduction of risk for contralateral breast cancer with adjuvant tamoxifen therapy. Five years is better then 2 yr or 1 yr of adjuvant therapy with tamoxifen (Fig. 4Go). In fact, 1 yr of adjuvant tamoxifen does not significantly reduce the incidence of contralateral breast cancer compared with control because the SD is so large (13 ± 13% reduction compared with control).



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Figure 4. The relationship between the duration of adjuvant tamoxifen and the reduction in contralateral breast cancer. A longer duration is clearly superior, and the 5-yr result that produces a 47% reduction in contralateral breast cancer is equivalent to the result observed in the tamoxifen prevention trial presented in Fig. 9Go. [Derived from Ref. 84.]

 
It is interesting to note that a quarter of the women allocated to the known adjuvant trials in the Overview Analysis (84) were Japanese, who have an annual incidence of contralateral breast cancer in patients not receiving tamoxifen of 2 per 1,000 compared with 6 per 1,000 elsewhere in the world. Therefore, if 5 yr of tamoxifen therapy can halve contralateral breast cancer, then the absolute benefit for Japanese women would be 1 per 1,000 and 3 per 1,000 elsewhere for both young and old women. Finally, the proportional reduction in contralateral breast cancer appears to be similar in women whose initial tumor being treated with tamoxifen was ER-poor (29 ± 15%) compared with the rest of the study population (30 ± 6%). This is an important result for the potential application of tamoxifen for the reduction of contralateral breast cancer in the woman with a primary breast cancer that is unequivocally ER negative.

C. Endometrial cancer
The overall increase in the incidence of endometrial cancer in the Overview Analysis was 2- to 3-fold (84). There was no association with dose, i.e., 20 mg and 30–40 mg daily produced relative risk (RR) ratios of 2.7 and 2.4, respectively. However, there was a suggestion that 1 and 2 yr of tamoxifen doubled the incidence of endometrial cancer and 5 yr quadrupled the incidence. However, the side effect is so rare (i.e., the numbers are too small) that the risk ratios are not significantly different from one another for each duration of tamoxifen. It is important, however, to state that the absolute increase in endometrial cancer was only half as big as the absolute decrease in contralateral breast cancer.

The Overview Analysis was able to identify 3,673 women who took 5 yr of adjuvant tamoxifen. With 26,400 woman years of follow-up before breast cancer recurrence in this group, there were seven endometrial cancer deaths. It is estimated that during the whole first decade, the cumulative risk was two deaths per 1,000 women. It is important to state that the current knowledge about the association of tamoxifen with endometrial cancer will improve these statistics. In general, the reported trials were conducted without awareness of the endometrial side effects of tamoxifen. This is no longer the situation, and early detection will improve mortality figures associated with tamoxifen.

D. Conclusions
Tamoxifen has been extensively tested in clinical trials of adjuvant therapy for 20 yr. The Overview shows that the proportional mortality reductions were similar for women with node-positive or node-negative disease (84). However, the absolute reductions in mortality were much greater in node-positive than node-negative disease. Additionally, patients with ER-positive disease have an increased reduction in death rate with longer duration of tamoxifen treatment, whereas patients who are ER-negative do not benefit from tamoxifen, regardless of the duration of therapy. The value of a long duration of treatment is most important for the premenopausal patient (Fig. 3Go). This latter finding is new, as the results for premenopausal women could not be ascertained with certainty in earlier overviews (74). The Oxford Overview Analysis has established the veracity of the laboratory concepts that tamoxifen would be most effective in ER-positive disease, longer duration would be more beneficial, and tamoxifen would prevent primary breast cancer, in this case contralateral disease (85, 86, 87, 88, 89, 90).

Overall, the absolute improvement in recurrence was greater during the first 5 yr after surgery, but improvement in survival increased steadily throughout the first 10 yr. This is an important finding because the patient is clearly benefiting from tamoxifen even after therapy has been discontinued. There is an accumulation of the tumoristatic/tumoricidal actions of tamoxifen for at least the first 5 yr of treatment, but the benefit continues after therapy stops. This is also true for the reduction in contralateral breast cancer; the breast seems to be protected so the value remains after therapy stops. This observation is extremely important for the application of tamoxifen as a preventive because a 5-yr course of tamoxifen would be expected to protect a woman from breast cancer for many years afterward.

Finally, the risk/benefit ratio of tamoxifen therapy can be stated to be strongly in the benefit category. The risk of endometrial cancer, a concept derived from laboratory studies (92), is of concern, but the benefits clearly outweigh the risks. In contrast, early concerns about the carcinogenic effects of tamoxifen in the rat liver (see Section VII) do not translate to the clinic as there is no evidence from the Overview Analysis of an increase in either liver or colorectal cancer in patients who take tamoxifen (84)


    IV. Selective ER Modulation
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 
Nonsteroidal antiestrogens were originally defined as compounds that would inhibit estradiol-stimulated rat uterine weight. The compounds tamoxifen (ICI 46,474) (93), nafoxidine (U, 11,100A) (94), nitromifene (CI628) (95), and clomiphene (MRL 41)(96) are all partial estrogen agonists in the uterus that also inhibit dimethylbenzanthracene (DMBA)-induced rat mammary tumor growth (67, 97, 98) and the growth of ER-positive MCF-7 breast cancer cell growth in vitro (99). Thus, in the 1960s and 1970s, antiestrogenicity was correlated with antitumor activity. However, the finding that the compounds expressed increased estrogenic properties, i.e., vaginal cornification and increased uterine weight in the mouse (93, 100), raised questions about the reasons for the species specificity. One obvious possibility was species-specific metabolism, i.e., the mouse converts antiestrogens to estrogens via novel metabolic pathways. However, no species-specific metabolic routes to known estrogens (101, 102) have been identified, but knowledge of the mouse model created a new dimension for study that ultimately led to the recognition of the target site-specific actions of antiestrogens. This concept was subsequently referred to as selective ER modulation (SERM) to describe the target site-specific effects of raloxifene, an antiestrogen originally targeted for an application in breast cancer but now used, paradioxically, as a preventive for osteoporosis (see Section VII). Now the whole class of drugs are known as SERMs.

The ER-positive breast cancer cell line MCF-7 (for a review see Ref. 42) can be heterotransplanted into immune-deficient athymic mice but the cells will only grow into tumors with estrogen support. Paradoxically, tamoxifen, an estrogen in the mouse, does not support tumor growth (103) but stimulates mouse uterine growth with the same spectrum of tamoxifen metabolites present in both the uterus and the human tumor (28). To explain the selective actions of tamoxifen in different targets of the same host, it was suggested that the ER complex could be interpreted as a stimulatory or inhibitory signal at different sites (28). The concept was consolidated with experimental evidence from two further models. First, tamoxifen and raloxifene maintain bone density in the ovariectomized rat, but both compounds inhibit estradiol-stimulated uterine weight (29) and prevent carcinogen-induced mammary tumorigenesis (30). Second, the finding that tamoxifen would partially stimulate the growth of a human endometrial carcinoma transplanted into athymic mice (91) allowed the investigation of two human tumors bitransplanted in the same mouse to determine whether tamoxifen could inhibit estrogen-stimulated growth of two tumors in the same host equally (92). Tamoxifen demonstrated target site specificity: breast tumor growth was controlled but endometrial tumors continued to grow. Again the range of tamoxifen metabolites were consistent in all target tissues despite the contrasting biological responses, so it was concluded that the ER complexes must be interpreted differently in different target tissues.

During the past decade an intense effort has been made to discover the reason for the target site-specific effects of antiestrogens. Not only will this knowledge permit a rational application of tamoxifen and raloxifene in patients, but also the discovery of new mechanisms for drug selectively will open the door for new innovations in drug discovery. For the sake of completeness, we will briefly consider some of the current hypotheses that could explain the molecular mechanisms of antiestrogen action in different tissue sites.

A. Antiestrogenic activity at the ER
The crystallization of the ligand-binding domain of the ER with estradiol and raloxifene has provided an important insight into the conformational changes that occur in the receptor (104) liganded with an estrogen or an antiestrogen, respectively. Estradiol causes helix 12 to seal the ligand inside the hydrophobic pocket of the ligand-binding domain (Fig. 5AGo). This causes receptor activation through the binding of coactivators on the surface of helix 12 (see Section IV.B). By contrast, the binding of raloxifene prevents helix 12 from sealing the hydrophobic pocket (Fig. 5BGo), and gene transcription cannot occur because coactivators cannot bind. Unfortunately, the final shape of the ER and anti-ER complexes do not tell us how the tertiary changes in protein structure occur. However, the crystal structure provides proof of the critical importance of AA351 (aspartate) for raloxifene action. The alkylaminoethoxy side chain is the essential structural feature of nonsteroidal antiestrogens (for a review see Ref. 105). The distance between the nitrogen and the oxygen must be optimal (106), the conformations available to the side chain must not be restricted (107), and the basicity of the nitrogen must be correct (108). Removal of the side chain results in loss of all activity or an increase in estrogenic properties (109). The side chain was originally predicted (110, 111) to bind to an "antiestrogenic region" in the ligand-binding domain of the ER to neutralize the estrogenic properties of the receptor. Simply stated, the antiestrogen was perceived to act like a stick to prevent the jaws of the ER from closing around the ligand. Looked at another way, an estrogenic complex would only be created by the protein enveloping the ligand. For the nonsteroidal antiestrogens, the "antiestrogenic region" is now known to be AA351 on helix 3. The discovery of an ER mutant in a tamoxifen-stimulated MCF-7 breast tumor (112) and the finding that it can increase the estrogenic properties of 4-hydroxytamoxifen (113, 114), the active metabolite of tamoxifen (115), and convert raloxifene from an antiestrogen to an estrogen (116, 117) is valuable biological proof that AA 351 is important for the antiestrogenic activity of these specific compounds. This interaction, at the critical contact point of helix 3 and helix 12, prevents helix 12 from sealing the ligand into the binding pocket (Fig. 5BGo). Interestingly, a recent report of the crystal structure of 4-hydroxytamoxifen and the ligand-binding domain of ER (118) shows a complex interaction of the side chain with several amino acids including AA 351. The distance between AA 351 and the nitrogen of 4-hydroxytamoxifen is further than the comparable interaction in raloxifene. This difference between the crystal structure of the raloxifene ER complex (104) and the 4-hydroxytamoxifen ER complex (118) may explain the promiscuous nature of the 4-hydroxytamoxifen ER complex. However, it must be stressed the AA 351 has no role for the antiestrogenic action of the pure antiestrogen ICI 182,780 (117) and other amino acids may be found to be involved in the antiestrogenic mechanisms of novel compounds in the future.



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Figure 5. Comparison of the binding of estradiol (diagram A) and raloxifene (diagram B) in the ligand-binding domain of the human ER. The key event is the repositioning of helix 12 to seal the steroid in the hydrophobic pocket. This event allows the ER complex to recruit coactivators for the transcription complex. The side chain of raloxifene prevents recruitment of coactivators by first masking AA 351 in helix 3, which is critical for the relocation of helix 12. Coactivators cannot bind to the AF2 region of helix 12 because it cannot seal the ligand-binding domain. [Derived from Ref. 104.]

 
Nevertheless, this two-dimensional model, which describes antiestrogen and estrogen action, is too simple to encompass all the complexities of the target site-specific actions of the antiestrogens. Furthermore, the crystallization data do not include the conformational information about the other half (i.e., the A, B, C, or F domains illustrated in Fig. 2Go) of the ER that control interaction with transcription factors and binding to DNA. Subtle changes in the whole protein shape could be responsible for changes in the intrinsic efficacy of the receptor complex at different target sites.

B. Coactivators for ER
A host of coactivator and corepressor proteins has been implicated in the construction of a transcription complex in target cells (63, 64, 119, 120, 121, 122, 123). The finding that an antiestrogen ER complex could become increasingly estrogenic in different cell contexts (124, 125) raised the possibility that the differential distribution of coactivators or corepressors could be responsible for changes in estrogenicity between the breast and, for example, bones (126, 127). The activating function (AF-2) region in the ligand-binding domain (Fig. 2Go) is known to be repressed by tamoxifen and raloxifene, but the AF-1 region is unaffected by tamoxifen binding (125). Clearly, the shape of a particular complex of ligand and ER will be different for different drugs (125). Thus coactivators could modulate estrogenicity differentially in different target sites. The candidate proteins could therefore amplify the anti-ER complex into an estrogenic complex. Alternatively, the anti-ER complex might recruit completely new proteins at a specific target site to induce or to suppress gene transcription.

C. Alternate response elements on DNA
Anti-ER complexes can bind to an estrogen response element but cannot recruit coactivators to initiate transcription of estrogen-responsive genes (114). However, it is possible that the anti-ER complex can bind to alternate sites in the promoter region to initiate transcription. Alu DNA repeats were originally thought to be functionally inert, but these elements may be able to activate gene transcription with antiestrogens through an ER-related mechanism (128, 129). Additionally, a specific region of the transforming growth factor-ß promoter is believed to be activated by raloxifene (130). A raloxifene response element has been identified (131), but the authors are now convinced that a simple protein DNA interaction does not occur (132).

D. An alternate ER-ERß
The discovery of a second ER, named ERß (133), has introduced a new dimension into the possible mechanisms of tamoxifen or raloxifene action. Although ERß has similar functional homology in the DNA-binding domain, there is only 55% homology between ERß and ER{alpha} in the ligand-binding domain. Clearly, one possibility to explain the target site specificity and altered estrogenicity of antiestrogens is a differential distribution of ER{alpha} and -ß to different tissues (134). The mechanism of action of the differential pharmacology between ER{alpha} and -ß may also involve different methods of gene activation. A novel signal transduction pathway has been identified as a protein-protein interaction between ERß anti-ER complexes and AP-1 (fos and jun) (135) that is capable of activating a reporter gene. Estradiol, however, does not activate the reporter. Therefore, the pathway would be of pharmacological rather than physiological significance. Interestingly, an ER{alpha} tamoxifen complex will activate AP-1 reporter systems in the context of an endometrial cancer cell (136). This has led to speculation that the target site specificity of antiestrogens could be both receptor and context selective.


    V. Biological Basis for Tamoxifen as a Breast Cancer Preventive
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 
With the molecular mechanisms of antiestrogens as a background, it is now appropriate to consider the scientific rationale for selecting tamoxifen to be tested as a breast cancer preventive, based on its pharmacological properties. Knowledge converged over the past 25 yr to make the choice of testing tamoxifen in well women a logical extension of clinical experience. Tamoxifen was selected for testing as a preventive based on 1) animal studies that demonstrated it could prevent carcinogenesis, 2) an extensive clinical experience that showed few serious side effects, 3) a beneficial profile of estrogen-like action in maintaining bone density, and 4) tamoxifen reduces circulating cholesterol. The fact that tamoxifen was already known to reduce the incidence of contralateral breast cancer made the drug the primary agent to test in high-risk women. The pharmacological properties of tamoxifen have been recently reviewed extensively (82); therefore, the purpose of this section is to act as a framework for a comparison with raloxifene in Section VIII and as a prelude to considering the current STAR trial (Section IX).

A. Animal models
Tamoxifen prevents rat mammary carcinogenesis induced by dimethylbenzanthracene, N-nitrosomethylurea, and ionizing radiation (86, 87, 88, 89, 30, 137), and long-term treatment prevents spontaneous carcinogenesis in C3H/OUJ mice infected with mouse mammary tumor virus (138). The latter result is of interest because tamoxifen is classified as an estrogen in the uterus and vagina of the mouse (93, 100). This, again, illustrates the target site specificity of tamoxifen in the mouse model, as mammary cancer is prevented almost completely. Although the athymic mouse model heterotransplanted with breast cancer cell lines has been extremely instructive for the use of therapeutic tamoxifen (103), and valuable as a model for understanding drug resistance (112), there are few parallels to chemoprevention. The MCF-7 cell line is derived from a pleural effusion, and the cells are, therefore, metastatic breast cancer (40). As such, the cell line does not replicate carcinogenesis in the breast or mimic primary breast cancer cells that have not developed the metastatic phenotype.

B. Bones
Tamoxifen maintains bone density in the ovariectomized rat (29, 31), and these observations have been translated to clinical trials. Sporadic reports (32, 139) and placebo-controlled randomized trials (33, 140) demonstrate that tamoxifen can increase bone density in the lumbar spine, forearm, and neck of the femur by 1–2%. Although the increases are modest compared with the results obtained with estrogen use or bisphosphonates ({approx} 5% increase in bone density), tamoxifen produced a significant decrease in hip and wrist fractures as a secondary end point in the breast cancer prevention trial (141). There is, however, a report that tamoxifen can reduce bone density in premenopausal women by 1–2% (142), but the decrease appears to be without clinical significance as there is no increase in the fracture rate. The reason for this may be the fact that a small decrease in bone density in a premenopausal woman is still well above the range of bone densities observed for women in their late 60s and 70s at risk for fractures.

C. Lipids
Tamoxifen reduces circulating cholesterol (34, 35). Low-density lipoprotein cholesterol is reduced by about 15%, but high-density lipoprotein cholesterol is maintained. It is hypothesized that this magnitude of fall in circulating cholesterol is a good surrogate marker for protection from coronary heart disease and atherosclerosis. In this regard there is evidence that woman who have been treated with 5 yr of adjuvant tamoxifen for breast cancer have a reduced incidence of fatal myocardial infarction (143, 144). Additionally, longer treatment (5 yr) appears to be superior to shorter treatment (2 yr) in reducing the number of hospital admissions for any cardiac condition (145). Conversely, a large study in the United States of five or more years of tamoxifen for the adjuvant treatment of breast cancer found no statistically strong evidence for the protection of women from coronary heart disease (146). Nevertheless, the incidence of coronary heart disease doubled once tamoxifen treatment was stopped, and, most importantly, there was no evidence for a detrimental effect of tamoxifen, i.e., tamoxifen did not increase the rate of coronary heart disease in pre- or postmenopausal women. The reasons for the disparate results probably reflect the populations studied. Breast cancer clinical trials usually require a good general health status before enrollment. Obviously, women at high risk for a second disease, coronary heart disease, would not be enrolled into a trial evaluating the efficacy of an antiestrogenic therapy in, for example, node-negative breast cancer where the prognosis, for the majority of women, would be expected to be good. Only a prospective randomized trial in a high-risk population would provide accurate data to support a claim for cardio-protection. At this point in time, there is no evidence that tamoxifen is detrimental based on current clinical evaluations, but there is no prospective clinical evidence that tamoxifen will reduce the risk of coronary heart disease.

D. Uterus
It is well known that tamoxifen produces a partial agonist action in the rat uterus (93), but the histology is different than the epithelial hyperplasia noted with estradiol (147). Until the late 1980s, there was very little information about the actions of tamoxifen in the normal human uterus. However, it is now clear that a variety of endometrial changes occur in unselected populations of women (148). The most significant finding is an increase in the stromal component rather than endometrial hyperplasia (149, 150). Despite the fact that tamoxifen has been used to treat endometrial cancer, the laboratory data suggesting that tamoxifen has the potential to encourage the growth of preexisting disease harbored in the uterus (91, 92) provoked an intense investigation of the rates of detection of endometrial cancer in women using adjuvant tamoxifen treatment for breast cancer. These data have been reviewed (151), and it is clear from the recent results of the tamoxifen prevention trial (141) that tamoxifen does not cause an excess of endometrial cancer in premenopausal women but does increase risk by 3- to 4-fold in postmenopausal women. This is consistent with the fact that women harbor 4–5 times the level of endometrial cancer than is detected clinically (152). In other words, the increase in the detection of endometrial cancer from 1 per 1,000 women per year to 3 per 1,000 women per year is consistent with the known rate of occult disease. Most importantly, the stage and grade of endometrial cancer observed in women taking tamoxifen is the same as those in the general population (141, 153).


    VI. Risk Factors for Breast Cancer
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 
If tamoxifen is an appropriate agent to test as a chemopreventive primarily because of its extensive clinical experience for the treatment of breast cancer, then the issue becomes identification of women at risk to select as a target population for recruitment to definitive clinical trials. Family history is probably the most well recognized risk factor for breast cancer, and it is now known that two forms of risk are associated with a family history of the disease. An inherited gene mutation predisposing to breast cancer is believed to account for only 5–10% of breast cancer cases (154, 155). Although infrequent, these mutations are significant since they are associated with a lifetime risk of breast cancer development of 50–80% (156, 157). At present, two predisposition genes, BRCA1, located on chromosome 17q21 (158), and BRCA2, located on chromosome 13q12–13 (159), have been identified. Both genes are inherited in an autosomal dominant fashion and are characterized by an extremely high risk of breast cancer development, which begins at a young age. Both genes also confer an increased risk of ovarian cancer development, which in BRCA1 carriers is estimated to be 10% by age 60 (160), and is lower in BRCA2 carriers. In addition, germ line mutations of the tumor suppressor gene p53, as seen in patients with the Li-Fraumeni syndrome, may account for about 1% of breast cancer cases occurring in women age 40 and younger (161, 162).

Most women with a family history of breast cancer do not have the genetically transmitted form of the disease, and therefore their increase in risk is much less than that seen in women who have inherited a predisposition gene. The cumulative probability that a 30-yr-old woman with a mother and sister with breast cancer will develop breast cancer by the age of 70 is reported to be between 7% and 18% (163, 164). While this risk increases as the number of relatives with breast cancer increases, the probability of cancer development if both a mother and sister have bilateral breast cancer has been reported to be only 25% (162, 164). The cumulative risk of breast cancer development in women with a family history of breast cancer rarely exceeds 30%, making it critically important to distinguish those women with hereditary breast cancer from those with a family history of the disease. Factors that should increase the clinician’s index of suspicion that a woman is at risk for genetically transmitted breast cancer include multiple relatives (maternal or paternal) with the disease, a family history of ovarian cancer in association with breast cancer, and a family history of bilateral and/or early onset of breast cancer. Although not all women with these factors will have genetically transmitted breast cancer, a referral for genetic counseling will allow the construction of a detailed pedigree to estimate both breast cancer risk and the competing causes of death due to an increased risk of the development of other types of cancer.

Breast cancer is clearly related to endogenous hormones, and numerous studies have linked breast cancer risk to the age of menarche, menopause, and first pregnancy. Although the absolute age-specific incidence of breast cancer is higher in postmenopausal than premenopausal women (165), the absolute rate of rise of the curve is greatest up to the time of menopause, and then slows to one-sixth of that seen in the premenopausal period. Further support for the promotional role of estrogen in breast cancer development comes from the observations that early menarche (166), late menopause (167), nulliparity, and late age at first birth (168) all increase the risk of breast cancer development. An increased number of ovulatory cycles is suggested to be the common mechanism of increased risk.

Other hormonal risk factors have been suggested but are not as well established. Abortion, whether spontaneous or induced, has been reported by some authors to increase risk (169, 170), while other studies have found no relationship between abortion and breast cancer risk (171, 172). Studies of the effect of lactation on breast cancer risk have also been inconclusive (173, 174), but recent studies have suggested that a long duration of lactation reduces breast cancer risk in premenopausal women (175). Physical activity in adolescence is reported to decrease risk, perhaps due to a higher rate of anovulatory cycles (176, 177), but an increased level of physical activity later in life has not been shown to reduce breast cancer risk (178). Postmenopausal obesity has also been shown to increase risk (179), perhaps due to increased peripheral estrogen production, but this relationship between weight and risk is not observed in premenopausal women. In fact, some studies have reported an inverse relationship between weight and risk at a younger age (180).

The effects of exogenous hormones in the form of oral contraceptives and hormone replacement therapy on breast cancer risk have been studied extensively, but few firm conclusions may be drawn. Overall, there is no convincing evidence of an increase in breast cancer risk in women who have ever used oral contraceptives (181). However, some studies have suggested that the long-term use of oral contraceptives in young women before first birth may increase breast cancer risk (182, 183). Two recent meta-analyses of the effect of estrogen replacement therapy demonstrate small but statistically significant increases in risk for users (184, 185). However, Steinberg et al. (184) noted no increase in risk until after at least 5 yr of estrogen use, after which a proportional increase in risk for each year of estrogen use was observed, while Sillero-Arenas et al. (185) did not observe a significant association between duration of hormone replacement therapy and breast cancer risk. In summary, although hormonal risk factors are clearly implicated in the pathogenesis of breast cancer, most of them are associated with a RR of 3 or less of breast cancer development (Table 4Go), and the presence of a single hormonal risk factor is insufficient to classify a woman as high risk.


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Table 4. Magnitude of known breast cancer risk factors

 
The relationship of benign breast disease to breast carcinoma was a subject of confusion for many years. The use of a standard classification of benign breast diseases as nonproliferative, proliferative, or proliferative with atypia has resolved much of the controversy. The histological diagnoses comprising these categories are shown in Table 5Go. Nonproliferative disease is associated with no increase in breast cancer risk, while proliferative disease increases risk by a factor of 1.5–2.0, and atypical hyperplasia by a factor of 4–5. Approximately 70% of palpable breast masses contain nonproliferative disease (186), and only 3.6% are atypical hyperplasia. The incidence of atypia is somewhat higher in biopsies performed for mammographic lesions, ranging from 7–10% (187, 188). However, the risk of breast cancer development 15 yr after a diagnosis of atypical hyperplasia is only 8% in the absence of a family history of breast cancer. Proliferative breast disease is also noted more frequently in women with a significant family history of breast cancer than in controls, further supporting its role as a risk factor (189).


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Table 5. Classification of benign breast disease

 
Another benign breast lesion that is clearly associated with an increased risk of breast cancer development is lobular carcinoma in situ (LCIS). In the past, LCIS was thought to be a malignant lesion, albeit one with a favorable prognosis. However, the finding that LCIS is associated with a risk of breast cancer development of approximately 1% per yr, the observation that the risk of carcinoma is equal in both breasts, and the finding that neither the extent of LCIS in the breast nor its presence at a margin of resection influence the risk of subsequent cancer have led LCIS to be regarded as a risk factor for breast cancer development rather than the actual precursor of carcinoma (190).

A number of environmental factors have also been linked to breast cancer risk. Exposure to ionizing radiation, whether secondary to nuclear explosion or medical procedures, has been clearly demonstrated to increase breast cancer risk (191, 192, 193). The level of risk varies with the age of exposure, with a minimal increase in risk observed for exposure in women older than 40 yr. A larger amount of attention has been directed toward the role of diet in the etiology of breast cancer. This link has been suggested by the large international variation in breast cancer incidence rates and the observation that national per capita fat consumption correlates with breast cancer incidence and mortality (194). However, prospective studies of diet and breast cancer risk have failed to identify a relationship between dietary fat intake and breast cancer incidence for up to 10 yr of follow-up (195). The lack of a relationship between dietary fat intake and cancer risk within the context of a Western diet is confirmed by a pooled analysis of seven cohort studies involving a total of 337,816 women, which demonstrated no difference in risk for women with the lowest and highest quintile of fat intake (196). However, all of these studies have addressed fat intake during adult life, and they do not exclude the possibility that fat intake during childhood and adolescence may influence subsequent breast cancer risk.

Stronger evidence exists to support an association between alcohol and breast cancer. A meta-analysis of 12 case control studies demonstrated a RR of 1.4 for each 24 g of alcohol consumed daily (197). Defining a relationship between age of alcohol consumption and breast cancer risk is more difficult, with conflicting data on the importance of drinking early in life (198, 199). A summary of the magnitude of increase in risk associated with the factors discussed is provided in Table 4Go.

A. Interactions among risk factors
A major problem in the clinical identification of the "high risk woman" is the lack of knowledge of the interactions among the various factors known to alter breast cancer risk, since the majority of studies have focused on defining individual risk factors. Most women have a combination of factors that both increase and decrease risk, complicating the assessment of an individual’s level of risk. In addition, it is unclear whether the risk conferred by multiple risk factors is additive, multiplicative, or varies with the risk factor under study.

The interactions between a family history of breast cancer and other risk factors have been examined, often with conflicting results. Dupont and Page (186) observed that the combination of atypical hyperplasia and a family history of a first-degree relative with breast cancer increased the RR of breast cancer to 11 times that of an index population, compared with a RR of 4.4 for atypia alone. However, Rosen et al. (200) found that the presence of a family history of breast carcinoma did not alter the level of risk after a diagnosis of LCIS, a lesion often considered part of a continuum with atypical hyperplasia. An analysis of data from the Nurses Health Study (201) found that in women with a mother or sister with breast cancer, known risk factors of age at menarche or menopause, parity, age at first birth, alcohol use, and the presence of benign breast disease did not further alter risk. In contrast, Anderson and Badzioch (202) and Brinton et al. (203) have reported that hormonal factors further modulate risk in women with a family history of breast cancer, although the effect varies with the factor under study.

Studies of the interaction between estrogen replacement therapy and other known breast cancer risk factors also have variable results, depending on the risk factor under study. In a meta-analysis of 16 published studies, Steinberg et al. (184) found that the effect of estrogen replacement did not differ among parous and nulliparous women and those with or without benign breast disease. However, an enhanced risk was observed in women with a family history of breast cancer. The analysis of the interaction among risk factors is further complicated by the fact that some factors may be important for the risk of premenopausal, but not postmenopausal, cancer and vice versa, and these effects may not be constant over time.

A model to predict the risk of breast cancer development in women at a given age over a defined time interval was developed by Gail et al. (204) using data from 4,496 matched pairs of cases and controls in the Breast Cancer Diagnosis and Demonstration Project. The model incorporates the risk factors of age at menarche, age at first live birth, number of first-degree relatives with breast cancer, and number of previous breast biopsies, and has been shown to predict risk accurately in two validation studies of women undergoing annual mammographic screening (205, 206). However, the model overpredicts breast cancer risk by 33% among women age 60 and younger who do not undergo annual screening. There are several other limitations of the model. Because only first-degree relatives are considered, it is not an appropriate model for women with extensive family histories of breast cancer, where risk may be underestimated. In women with risk due to LCIS or atypical hyperplasia, the model underestimates risk, since the highest RR for breast biopsy is 2.0. Similarly, for the woman with nonproliferative disease, the model may overestimate risk. In spite of these limitations, the model is a clinically important tool for identifying a woman’s level of risk over a clinically relevant time period, after correction for competing causes of mortality. The Risk Disk, which is available from the National Cancer Institute, uses the Gail model to provide a numeric estimate of a woman’s 5 yr and lifetime risk of developing breast cancer compared with an "average risk" woman of the same age. Examples are given in Figs. 6Go and 7Go.



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Figure 6. Risk assessment for breast cancer in a woman with two risk factors. The woman illustrated here has a mother with breast cancer and has never had any children. The combination of these factors means that her 5-yr risk of breast cancer development is 1.6%, compared with 0.5% in a woman with no risk factors. If she lives to the age of 70, her risk will be 18% compared with 6.4% for the woman with no risk factors. This level of risk would not have qualified the woman to participate in the recently completed Breast Cancer Prevention Trial with tamoxifen.

 


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Figure 7. Risk assessment for breast cancer in a woman with multiple risk factors. The woman illustrated here has early menarche, late age at first birth, and has a mother and sister with breast cancer. In addition, she has had a breast biopsy showing atypical hyperplasia. This combination of risk factors makes her 5-yr risk of breast cancer development 7.5% and her lifetime risk 53.4%. She is an ideal candidate to consider tamoxifen for risk reduction.

 
B. Identification of candidates for chemoprevention
Women at increased risk for breast cancer would seem to be ideal candidates for chemoprevention initiatives. However, from the preceding discussion it is apparent that the problem of identification of the high-risk woman is far from solved. There is no consensus regarding what level of increase in risk is clinically relevant. The interactions among risk factors and their variability over time are poorly understood, and most of the data on risk come from studies of white women, so little is known about the impact of ethnic diversity on risk. Finally, with the exception of women with mutations of breast cancer predisposition genes, the majority of women with risk factors will not develop breast carcinoma. In addition, a recent study of the fraction of breast cancer cases in the United States due to attributable risk factors (207) found that fewer that 50% of women who develop the disease have any identifiable risk factors. A family history of breast cancer accounted for only 9.1% of cases, while relatively minor risk factors such as later age at first birth and nulliparity contributed 29.5% of cases. In a similar study, Seidman et al. (208) noted that only 21% of breast cancer cases in women age 30–54 and 29% of cases in women age 55–84 occurred in women with 1 of 10 common breast cancer risk factors. The majority of women in the studies described had minor risk factors, which increased the RR of breast cancer only 2-fold, and most had only a single risk factor. This level of "increased risk" would not meet the entry criteria for the trials of breast cancer prevention in high-risk women discussed below. These data suggest that even if women with a very small increase in breast cancer risk were targeted for prevention initiatives, a large number of cases would continue to be missed.


    VII. Prevention of Breast Cancer with Tamoxifen
 Top
 Abstract
 I. Introduction
 II. Lacassagne’s...
 III. Tamoxifen as an...
 IV. Selective ER Modulation
 V. Biological Basis for...
 VI. Risk Factors for...
 VII. Prevention of Breast...
 VIII. Biological Basis for...
 IX. Study of Tamoxifen...
 X. The Future of...
 Dedication and Acknowledgment
 References
 
This section will explore progress that has been achieved in the last decade to answer the question, "Does tamoxifen have worth in the prevention of breast cancer in high risk women?". Two studies have claimed to address this question—The Royal Marsden Pilot Study (209) and the National Surgical Adjuvant Breast and Bowel Project (NSABP) Protocol P-1 (141). However, the Marsden study was not designed to answer the question about the prevention of breast cancer. It was a pilot toxicology study (142) that was subsequently part of a nationwide clinical trial in Britain that planned to recruit a total of 20,000 high-risk women. The main British study is ongoing. Additionally, an Italian report of the efficacy of tamoxifen in a small number of low-risk women (~5,000) has been published (210), but again this is a small component of a 20,000-volunteer trail that has now been stopped.

A. Royal Marsden Pilot Study
Powles and co-workers (211) recruited high-risk women aged 30–70 to a placebo-controlled trial using 20 mg of tamoxifen daily for up to 8 yr. Women were eligible if their risk of breast cancer was increased due to family history. Each participant had at least one first-degree relative with breast cancer under age 50, or a first-degree relative affected at any age plus an additional affected first- or sec